Objective To investigate the effects and the possible mechanism of insulin like growth factor-1 ( IGF1 ) on proliferation and migration of human umbilical vein endothelial cells ( HUVEC) in high glucose. Methods Cultured HUVEC were divided into 5 groups( control group, high glucose group, hypertonic group, high glucose+IGF 1 group, high glucose+IGF1+ AZD5363 group) . The proliferation was detected by methyl thiazolyl tetrazolium ( MTT) , the migration was detected by transwellchamber. The mRNA expressions of serine/threonine kinase( Akt) and FoxO1 were detected by real-time PCR. The protein expressions of FoxO1/p-FoxO1 and Akt/p-Akt were detec-ted by immunohistochemisty. Results Compared with the control group, the rate of cell survival in high glucose group decreased significantly(P<0. 05), the transitional was decreased significantly(P<0. 05), the mRNA ex-pression of FoxO1 was increased significantly( P<0. 05 ) , the protein expressions of FoxO1/p-FoxO1 and Akt/p-Akt were increased. Compared with high glucose group, the cell survival rate and migration rate in high glucose+IGF1 group were increased significantly, the mRNA expression of FoxO1 was decreased significantly(P<0. 05), the protein expressions of FoxO1/p-FoxO1 and Akt/p-Akt were decreased. Compared with high glucose group, the cell survival rate and migration rate in high glucose+IGF1+AZD5363 group had no significant difference, the mR-NA expression of FoxO1 was decreased significantly ( P<0. 05 ) , the protein expressions of FoxO1/p-FoxO1 had no significant difference. The mRNA expression of Akt in five groups had no significant difference. Conclusion IGF1 can improve the proliferation and migration of endothelial cell in high glucose. It shows that IGF1 acts through Akt to promote FoxO1 phosphorylation.

Objective To analyze syndrome types and TCM prescriptions of Behcet's disease;To summarize the regularity of TCM syndrome differentiation and treatment principles of Behcet's disease.Methods Related literature in CNKI and VIP database from Jan. 1990 to Oct. 2013 was searched. The syndrome patterns, characteristics of prescriptions and frequency of syndrome were summarized.Results There were 20 syndrome types of Behcet's disease in the 58 collected literature. Damp and hot accumulating poison, spleen and kidney Yang deficiency, liver and kidney Yin deficiency, and hot and damp were common syndromes, occupying 55.4%. Hot and damp occupied 41.3%;Yin deficiency occupied 18.1%;Yang deficiency occupied 12.9%. Liver, spleen and kidney were the main disease locations, occupying 94.4%. There were 197 Chinese herbs in total, and could be classified and further combined into 19 types, among which heat-clearing drugs, tonics, activate blood and removing blood stasis were commonly adopted, occupying 50.6%. There were 66 prescriptions collected, among which 31 were set prescriptions, and 35 were prescriptions formed by self-experience. Longdan Xiegan Decoction and Gancao Xiexin Decoction were commonly adopted.Conclusion Syndrome types and TCM prescription characteristics of Behcet's disease had certain regularity, which can provide reference for clinical diagnosis and treatment.

Objective To investigate the effect of early goal lactate clearance rate therapy in patients with severe sepsis or septic shock.Methods Articles were retrieved from PubMed,Embase,Cochrane Library,Wanfang and CNKI before March 5,2016.Inclusion criteria included the subjects concerning patients with severe sepsis or septic shock reported as randomized controlled trial (RCT),which endpoints were the mortality,the length of ICU stay and hospital stay.RevMan 5.3 software was used for Meta analysis.Results There were 5 RCTs meeting inclusive criteria including 860 patients.It was shown by Meta analysis that early goal lactate clearance rate therapy was associated with decrease in the 28-day mortality(RR =0.73,95％ CI:0.60-0.88,P ＜ 0.01),shorten the length of ICU stay (WMD =-2.41,95％ CI:-4.68--0.14,P ＜ 0.05),but not associated with decrease of the length of hospital stay(WMD =-0.13,95 ％ CI:-4.58-4.31,P =0.95).Conclusion Early goal lactate clearance rate therapy was associated with significant improvement in 28-day mortality and the length of ICU stay but not with the length of hospital stay in patients with severe sepsis or septic shock.

Objective To investigate the correlation of the single nucleotide polymorphism (SNP) of PON1 (Paraoxonase-1) gene rs854572 to the occurrence of clopidogrel resistance (CR). Methods A case-control method was employed in present study. A total of 850 hospitalized patients with coronary artery diseases (CAD) in General Hospital of Shenyang Command were enrolled. The residual platelet aggregation rate (RPA) induced by 20μmol/L of adenosine diphosphate (ADP) was detected by optical nephelometry, and RPA≥70% was defined as CR. Accordingly, all the enrolled 850 patients were then divided into CR group (n=215) and non-CR (NCR) group (n=635). Polymerase chain reaction (PCR) and pyrophosphate sequencing were executed to determine the genotypes and the allele frequencies of PON1 gene rs854572. Results The genotype frequencies in rs854572 of PON1 gene conformed well to the Hardy-Weinberg equilibrium in both CR group and NCR group. Three frequencies of genotype CC, CG and GG were 23.7%, 49.3%, 27.0% in CR group, and 24.1%, 50.2%, 25.7% in NCR groups, respectively. No significant difference in genotype and allele frequency existed between CR group and NCR group (P=0.93 and 0.76, respectively). Logistic regression analysis revealed that no correlation between rs854572 SNP of PON1 gene and the formation of CR in patients with CAD after adjustment of correspondent factors including age, gender, body mess index, smoking, hypertension, diabetes mellitus and hyperlipidemia. Conclusions It is considered that no correlation exists between PON1 gene rs854572 polymorphism and clopidogrel resistance in patients with coronary heart disease.

BACKGROUND:Studies have confirmed that nicotine affects the activity of osteoblasts, osteoclasts, fibroblasts and erythrocytes.
OBJECTIVE:To study the nicotine effects on osseointegration and the expression of osteoprotegerin and bone morphogenetic protein 2 after implantation of dental implants with surface treatment by sandblasting or acid etching.
METHODS:Twenty-four Sprague-Dawley rats were randomly assigned to two groups and received daily injections for 2 weeks as folows: Nicotine 2 mg/kg twice for experimental group, saline solution for control group. Then the titanium implants with surface sandblasting or acid etching were implanted into the tibiae folowed by continuous nicotine or normal saline injection. At weeks 2 and 4 after implantation, the implants and surrounding bone tissue were prepared for CT, X-ray and hematoxylin-eosin staining examinations to evaluate bone healing and expression levels of bone-related genes were measured by quantitative RT-PCR.
RESULTS AND CONCLUSION: Compared with the control groups, the degree of osseointegration and the expression of osteoprotegerin and bone morphogenetic protein 2 in the experimental groups were decreased significantly (P < 0.05), except that the expression of bone morphogenetic protein 2 in the experimental group with acid etching was not significantly reduced. In addition, the expression of bone morphogenetic protein 2 in the experimental group with acid etching was higher than that in the experimental group with sandblasting at 2 weeks after implantation (P < 0.05). The X-ray and CT show that the quantities of new generation bone and the degree of bone mineralization of the sandblasting group were significant lower than those of the acid etching group under the intervention of nicotine. Hematoxylin-eosin staining showed that the activity and quantity of osteoblasts around the implants down-regulated significantly, but acid etching-treated implants showed better outcomes than sandblasting-treated implants.

OBJECTIVE:To reduce the risk of single dispensing,and to improve the quality of pharmaceutical care in emergen-cy pharmacy. METHODS:Single dispensing environmental factors,human factors and social factors of emergency pharmacy in our hospital were analyzed;the suggestions and measures were put forward,risk intervention mechanism was established and manage-ment effects were evaluated. RESULTS:Through improving the pharmacy hardware and software,carrying out the relevant sys-tem,enhancing training,improving the process of dispensing and window order,promoting the drug management and carrying out near error management. Compared with before management(Oct.-Dec. 2014),the times of near error and disputes significantly re-duced after management(Feb.-Apr. 2015)(P<0.05),and medication error reduced to zero. CONCLUSIONS:As single dispens-ing,risk management and the improvement of prescription audit ability can improve pharmaceutical care in emergency pharmacy.

Objective To explore the association between the polymorphism in IL-28B gene and the interferon virological response in HBeAg-posi-tive chronic hepatitis B patients. Methods A literature search was performed on PubMed,web of Science,China Bio Medicine(CBM)and wan-Fang database. The references was screened with the inclusion and exclusion criteria,and then data were extracted and methodological quality of the studies was judged. Statistical analysis was carried out with RevMan 5.3 and STATA 11.0. Results Totally 7 publications were recruited for the study,including 1 345 patients. The meta-analysis showed that there was no significant difference of interferon response between HBeAg-positive chronic hepatitis B patients with rs8099917TG/GG alleles and rs8099917TT alleles (P= 0.20). There was no statistical difference between Rs12979860CC and CT/TT allele for the sustained virological response(P=0.75). Conclusion Our current meta-analysis suggests that there was no correlation of rs8099917,rs12979860 polymorphism in IL-28B with the sustained virological response to interferon in HBeAg-positive chronic hepatitis b.

he blood supply of corpus callosum is rich, its function is complex, and the incidence of infarction is low. The risk factors and etiology for callosal infarction do not have any difference with the infarction in other parts of the brain. The clinical manifestations of the disease are complex and diverse. The two classical clinical manifestations are callosal disconnection syndrome and frontal-type gait disorder, but hemiplegia, monoplegia, apraxia, and mental retardation are common in clinical practice. The positive rate of CT scan is lower. MRI has higher sensitivity and specificity for callosal infarction. The diagnosis of callosal infarction is not difficult according to the history and imaging examination, however, it needs to be differentiated with other diseases that likely involve corpus callosum. Most of the patients with callosal infarction have good prognosis.

Objective To investigate the drinking water quality of secondary water supply in Tongzhou District, Beijing in order to establish a data monitoring management system. Methods The uniform questionnaires were used for the on-the-spot investigation at secondary water supply units in the whole district, which included secondary water supply facility situations, environmental sanitary conditions and health management and so on. At the same time, municipal water and secondary supply water were tested. Results 48 units of secondary water supply were investigated. In this investigation, the main systems of secondary water supply were the low water tank added frequency conversion pump(accounts for 77.1%) and the inverter constant pressure water supply system with negative pressure(accounts for 14.3%). According to the hygienic requirements, the unqualified rate of water tank structure, disinfection facility and water tank room were 81.0%, 73.3% and 87.3% respectively; besides, 39.6% of the units used the invalid hygienic licenses, 43.5% of the water managers had no health certificates, and 54.5% of the units had never cleaned their water tanks. The total qualification rate of the secondary water supply was 66.7%. The main items that were not up to the standard were ammonia-nitrogen, free chlorine, recognizable objects with naked eyes, total bacteria count and turbidity. For the different water material, the qualification rate showed a significant difference(P=0.023). Conclusion The drinking water quality of the secondary water supply is lower than before and the preventive hygiene supervision and evaluation is comparatively delayed in the investigated district.