Alphapapillomavirus ; physiology ; China ; Global Health ; Humans ; Papillomavirus Infections ; virology

Objective To explore the neuroprotective effect of magnesium sulfate on radiation induced neural stem cell injury. Methods Brain tissue was obtained from new-born sprague-dawley rats within 24 hours, and the cerebral hemisphere was dissociated to culture the neural stem cells. After being identified by immunofluorescence method, the neural stem cells were randomly divided into 3 groups as blank control group, experimental control group and experimental group. The neural stem cells of experimental control group and experimental group were irradiated with 2 or 4 Gy of gamma rays. The proliferation and the cell cycle of neural stem cells were detected at different time-points ranging from 24 h,48 h, 72 h after irradiation with CCK-8 and FCM. Results Compared with the blank control group, the proliferation rate of experimental control group was significantly reduced ( t = 5. 33-8.44, P ＜ 0. 05 ), and the G1 phase arrest of experimental control group was significantly enhanced ( t = 30. 60-71.22, P ＜ 0. 05 ).Compared with the experimental control group, the proliferation of experimental group significantly increased excluding that of 24 h ( t = 2.45-4. 71, P ＜ 0.05 ), the apoptosis rate of experimental group significantly decreasd (t = 6.73-41.12, P ＜ 0.05 ), which was closer to the blank control group.Conclusion Magnesium sulfate can alleviate the injury of proliferation and decrease the cell apoptosis in the early stage after irradiation.

Objective To investigate the effect of resveratrol ( Res ) on the expression of survivin, p27 protein in human renal cell carcinoma 786-0 cells. Methods The cell cycle and apoptosis of 786-0 cells were detected by flow cytometry, and the expression of survivin and p27 protein of 786-0 cells was detected by Western blotting at time point of 12, 24 and 48 h after human renal cell carcinoma 786-0 cells were cultured with Res ( 25μmol·L-1 ) and blank medium, respectively. Results The ratio in G1 phase [(64.87±3.76)%] and G2 phase [(9.91±0.82)%] in the experimental group decreased significantly (P<0.05), and the ratio of S phase [(25.05±3.12)%] increased significantly (P<0.05).The apoptosis rate of experimental group at 12, 24 and 48 h [(5.12±0.64)%, (5.71±0.49)%, (5.84±0.30)%] was significantly higher than that of the control group [ ( 0. 72 ± 0. 27 )%, ( 0. 93 ± 0. 25 )%, ( 1. 12 ± 0. 21 )%, P<0. 05 ] . The expression of survivin protein in experimental group was significantly higher than that of the control group ( P<0. 05 ) , and the expression was significantly increased with time prolonging (P<0.05).The expression of p27 protein in experimental group was significantly lower than that of the control group (P<0.05), and the expression level was significantly decreased in time-dependent manner (P<0.05).There was a negative correlation between the expression of survivin and p27 protein in the experimental group ( P<0.05) . Conclusion Res could inhibit the expression of p27 protein and up-regulate survivin expression level, and promote the apoptosis of renal cell carcinoma 786-0 cells.

Through approaches of literature statistics and content analysis, the English translation studies of package instruction of Chinese medicine from Chinese literature database were summarized and analyzed, including China National Knowledge Infrastructure (CNKI), Wangfang Data and VIP Database. It was found that there existed four main problems in English translation of package instruction of Chinese medicine. First, there were relatively few studies in this field. Second, the papers were distributed unevenly in the relevant periodicals. Third, most studies were practice-based studies. Fourth, the research methods were simple. Theoretical guidance, research system and empirical research should be paid attention in English translation studies of package instruction of Chinese medicine.

Objective:To evaluate the periodontal tissue regeneration effect of implantation of bone marrow stromal cells(BMSCs)-root-coralline hydroxyapatite(CHA) complex.Methods:BMSCs of beagle dogs were in vitro cultured on CHA scaffold and on tooth root surface respectively.Then BMSCs-root samples were inserted into BMSCs-CHA scaffolds to form BMSCs-CHA-root complexes.5 complexes were implanted on one side of mandible in each of the dogs 1 month after the 4th,5th and 6th teeth had been extracted.Root-CHA without BMSCs was implanted on another side as the control.All dogs were killed 16 weeks after implantation,and the periodontal tissue regeneration were observed histologically.Results:More new bone formation and more mature alveolar bone were observed in the test group than that in the control,and there was a clearly periodontal ligament-like tissue formation only in the test group.Conclusion:The complex of BMSC-root-CHA can improve the periodontal tissue regeneration.

Objective To evaluate the usefulness of bilateral internal iliac arterial embolization in the treatment of hemorrhagic cystitis after bone marrow transplantation. Methods Among 28 patients with hemorrhagic cystitis, 7 patients experienced bilateral internal iliac artery embolization because of serious hemorrhage that couldn′t be controlled by conservative treatments,including CML (n = 3) , AML(n = 2) , ALL (n = 1),acute myelomonocytic leukemia(M4, n = 1). Embolization of the target vessels was performed by using gelfoam particleas for bilateral internal iliac arterial visceral branches. Results Gross hematuria disappeared in 4 cases after embolization by gelfoam particles. The time of bleeding controlled continued 2-10 d(mean 6 d). No rebleeding was observed in all of the cases during the 12 - 82 days follow up.Gross hematuria markedly reduced in another 3 cases after embolization. But failure occurred in other two patients. No definite complication related to the procedure was noted. Conclusion Bilateral internal iliac arterial embolization is an effective, less invasive, and safe method for serious hemorrhagic cystitis after bone marrow transplantation. (J Intervent Radiol, 2006, 15: 267-269)

OBJECTIVE:To establish the HPLC method for determination of tanshinone Ⅱ A in compound Jinpu tablets METHODS:HPLC was performed on Shim-pack CLC-ODS(4 6mm?250mm,5?m) with methanol-water(15∶5) as mobile phase Flow rate was 1 5ml/min and detecting wavelength was 270nm Tanshinone Ⅱ A in compound Jinpu tablets was extracted by reflux method RESULTS:The calibration curve was linear in the concentration range of 0 01 333～0 10 667?g/ml(r=0 9 998,n=5) CONCLUSION:This method is simple,accurate,repeatable and effective for quality control of compound Jinpu tablets

OBJECTIVE To investigate the infection status and drug resistance of Mycoplasma strains isolated from genitourinary tract. METHODS The IST kit produced by Bio-Merieux was used to culture the Mycoplasma strains and to do drug susceptibility test. RESULTS The Mycoplasma infection ratio was 41.0%,with Ureaplasma urealyticum(Uu) 71.9%,Mycoplasma hominis(Mh) 2.3%,and mixed infection 25.8%,respectively.The drug susceptibility test showed that drug resistance ratios were as follows: to erythromycin 58.7%,ciprofloxacin 50.0%,ofloxacin 41.3%,pristinamycin 3.3%,josamycin 5.4% and doxycycline 7.6%. CONCLUSIONS Mycoplasma infection in genitourinary tract is mainly due to Uu infection.We should rationally choose and use antibiotics according to the drug susceptibility,and josamycin is very effective.

To study the effects of the material of a buildup cap to the determined value of Output FactorSc．Material and MethodsUsing two buildup caps made of plastic A－150 and copper respectively．The ionizing values of ionization chamber were measured with Farmer 2570 electrometer in the fields of all kinds of sizes respectively and calibrated to reference field．Two groups of values of Sc were obtained and compared． Results The ionizing values measured with the buildup cap of copper is evidently larger than with the buildup cap of plastic A－150 and the maximum is 14％ but the difference between two groups of the values of Sc is not much evident and all is in the range of 1％． Conclusions Though the material of high density is not equivalent with airthe effects of that to the measured value of Sc is less．So using the buildup cap made of the material of high density for the measurement of Sc is also a quite good method specially in the fields of the small sizes．

Based on the existing situation of preventive medicine in our country,this article gives insights into the problems and the resolves of college preventive medical education in the field of society,colleges,teaching materials,teachers,students and teaching conditions.