Emodin is the main effective monomer of Rhubarb, which has a variety of pharmacological actions and valuable clinical applications.Recently, there are many reports about molecular mechanisms of emodin pharmacological actions,and they givehighly attention on the dephlogisticating and antineoplastic effects. These effects are achieved through affecting the concentration and transportation of ions, resisting of oxidation and free radical, and affecting the secretion of inflammatory factor and the enzyme activity and apoptosis and so on. This article summarizes the reports and hopes to provide theoretical basis for the practical application.

Objective:To investigate the association between anxiety and the change of humoral immune functions and its correlation with HLA-DQB1 polymorphisms.Methods:Total 31 resident doctors were selected randomly and tested by State-Trait Anxiety Inventory(STAI).IgG,IgA,IgM,complement C3 and complement C4 were detected with BECKMAN array360 system;HLA-DQB1*02、*03、*04、*05 and*06 alleles were individually amplified by polymerase chain reaction(PCR)using exon2 group-specific primers.The correlation between immune function and HLA-DQB1 polgmorphisms were investigated.Results:Statistical analysis showed that there was positive correlation with State Anxiety (Ta) and complement C3,either Trait Anxiety (Tc) and complement C3.There was significant difference between HLA-DQB1*02 positive and negative in Ta (P＜0.05),while no difference in complement C3(P＞0.05).There was significant difference between HLA-DQB1*04 positive and negative in Ta and Tc(P＜0.05),while no difference in complement C3(P＞0.05).Conclusion:Anxiety could change some humoral immune functions and this is related with HLA-DQB1 polymorphism.

To investigate the correlation between traditional Chinese medicine (TCM) syndrome and short-term prognosis of ischemic stroke.

Objective To explore the experience of surgical treatment for appendiceal carcinoid.MethodsThe clinical data of 12 appendiceal carcinoid cases received surgery were retrospectively studied.ResultsPreoperatively,all the 12 cases were considered as appendicitis.The definite diagnosis was verified by pathologic study postoperatively.Among the 12 cases, tumors less than 1 cm 9 cases, between 1 cm to 2cm 2cases, but without serosal involvment or lymph node metastasis(2 cases) recieved simple appendectomy.One case with a tumor larger than 2cm and serosal involvment underwent fight hemicolectomy.ConclusionOwing to its lack of specific symptoms,the diagnosis of appendiceal carcinoid was usually reached after operation.The choice of surgical procedures should based on the size and depth of invasion of the tumor and lymph node metastasis status.

Colorectal neuroendocrine neoplasm (NEN) is originated from colorectal neuroendocrine cells.The incidence of colorectal NEN is low,however it has been increasing in recent years.As the research work has gone further,WHO has graded colorectal NEN to neuroendocrine tumor,neuroendocrine carcinoma,mixed gland-neuroendocrine carcinoma and hyperplastic and pre-neoplastic lesions.Although colorectal NEN cells can produce hormones and have endocrine functions,the clinical symptoms may not be shown.The diagnosis and treatment for colorectal NEN are different according to different classifications and stages.

Objective:To explore the relationship between carotid atherosclerosis (CAS)detected by ultrasonography and ECG abnormality and correlation between CAS and blood pressure in patients with coronary heart disease (CHD).Methods:A total of 389 CHD patients were divided into CAS group (n=213)and no CAS group (n=176).ECG abnormalities were compared between two groups,and ECG and blood pressure were compared among patients with different degrees of CAS in CAS group.Results:Compared with no CAS group,there were significant rise in constituent ratios of atrial fibrillation,Ⅱ~Ⅲ°atrioventricular block,total lead number of ST segment de-pression,and of abnormal T wave,ST segment deviation complicated abnormal T wave and angina pectoris compli-cated ST segment depression in CAS group (P <0.05~<0.01).Along with CAS grade increased (grade I~ grade IV),there were gradual rise in percentages of atrial fibrillation (8.33% vs.21.33% vs.31.03% vs.45.00%),to-tal lead number of ST segment depression (17.64% vs.27.22% vs.37.07% vs.52.08%),total lead number of ab-normal T wave (5.00% vs.6.56% vs.10.34% vs.22.08%)and ST segment deviation complicated abnormal T wave (26.67% vs.37.33% vs.50.00% vs.60.00%),P <0.05 or <0.01. There were no significant difference in diastolic blood pressure (DBP)between no CAS group and CAS group,but systolic blood pressure (SBP)and pulse pressure (PP)of CAS patients were significantly higher than those of no CAS patients,and they gradually elevated along with CAS severity increased (P <0.05~<0.01).Pearson correlation analysis indicated that CAS was signifi-cant positively correlated with SBP and PP (r =2.064,1.348,P <0.01 both).Conclusion:The incidence rate of abnormal ECG in CHD patients complicated CAS is higher than that of CHD patients without CAS;and CAS is pos-itively correlated with blood pressure.Therefore,carotid ultrasonography can better reflect CHD patients′condition.

Surgery in combination with chemotherapy and radiotherapy is the standard of lung cancer treatment,but postoperative recurrence is very common which usually leads to higher mortality and lower life quality. Immunotherapy on post-operative patients fully mobilizes the body's defense mechanisms,activates the immune cells,and kills residual cancer cells. Current research on lung cancer immunotherapy mainly includes four categories:adoptive immunotherapy,dendritic cell vaccine,non-specific antigen immune therapy and anti-gen-specific vaccine. These studies show lung cancer immunotherapy intervention can effectively reduce postop-erative residual cancer cells,reduce postoperative recurrence rate,prolong survival,significantly improve the prognosis,and is worth spreading in clinical practice.

Objective To prepare the long‐term use and good stability of HBsAg feeble masculine serum ,and the application of controlling quality for HBsAg .Methods The HBsAg positive serum was diluted using calf serum phosphate buffer by grads ,multi‐ple to 1∶2 ,1∶4 ,1∶8 ,1∶16 ,1∶32;HBsAg feeble masculine serum was detected by double antibody sandwich method of enzyme‐linked immunosorbent assay (ELISA) using different reagent lot obtained from two companies for continuous six months .Results The serum ,s diluting multiple which the S/CO ,value between 1 to 3 was 1∶8 ,and the S/CO was 2 .42 ,the CV was 4 .72% .The serum of quality control possessed a good stability in different detection reagents ,the S/CO value range from 2 .41 to 2 .82 ,and the CV value was 4 .24% to 8 .68% .The CV value of HBsAg feeble masculine control serum with 6 months successive detection ranged from 8 .71% to 9 .86% (CV<10% ) .Conclusion The HBsAg feeble masculine serum possessed good stability and precision which is fit for masculine serum in HBsAg test .

Objective:To establish a detection method for residual toluene in diclofenac sodium raw material by GC. Methods:An HP-5 column (0. 53 mm × 30 m, 2. 65 μm) with nitrogen as the carrier gas was employed for the separation. The temperature of the column, injection port and FID detector was set at 50℃, 200℃ and 250℃, respectively. An internal standard method with isooctane as the internal standard was used to assay the residual amount after the sample was dissolved in DMSO. Results:The linear calibration curve was obtained over the range of 9. 27-111. 19μg·ml-1(r=0. 999 4). The mean recovery was 97. 27%(RSD=2. 80%,n=9). The LOD and LOQ was 0. 59μg·ml-1 and 1. 46μg·ml-1 , respectively. The residual contents of toluene in 3 batches of the products all met the relevant regulations. Conclusion:The established method is reliable and accurate, and can be used in the quality control of diclofenac sodium.