Percutaneous vertebroplasty (PVP) and kyphoplasty (PKP) are perf or med by image-guided spinal procedures and consists in percutaneously transpedic ular injection of the polymer polymethylmethacrylate (PMMA) into a fractured ver tebral body to relieve the pain and to strengthen the bone. Advantages of the te chnology include its relative simplicity, fewer complications and minimally inva sive surgery. PKP can also restore vertebral height. This paper is to sum up the results of biomechanical comparison of different cements, different volumes and different distribution in the vertebras. This article also indicates the disadv antages of PVP and PKP at present. To achieve the goal in the future, the operat ions and ideal biomaterials should be improved and developed.

Objective To investigate the expression and localization of S100B protein in the placenta and umbilical cord tissue of the pregnant women at different gestational ages. Methods The placenta and umbilical cord tissues were obtained from 60 healthy pregnant women at different gestational ages:13～27 weeks (a = 20),28～36 weeks (n = 20),37～41 weeks (n = 20). The localization and expres-sion of S100B protein was detected by S-P immunochemical method and Western blotting. Results In the placenta,the S100B protein lo-cated in the trophoblast.myofibroblasts.macrophages and smooth muscle cells. The intensity of immunostaining and protein concentration in-creased with advancing gestation. In the umbilical cord,the S100B protein was found in the smooth muscle cells,myofibmblasts,amnion ep-ithelium, macrophages and monocytes. S100B piotein expression showed no significant difference in the different cells of the umbilical cord. Conclusion S100B protein expression in the placenta and umbilical cord tissues is throughout the gestation and increases with advancing gestation in the placenta.

Exogenous surfactant replacement has been successfully achieved and become standard therapy in neonatal respiratory distress syndrome,but clinical trials in acute respiratory distress syndrome (ARDS) had mixed results.Early studies show that pulmonary surfactant(PS) administration in ARDS can significantly improve oxygenation and decrease mortality,but in recent years,some clinical trials haven't achieve the most substantial benefits to long-term patient outcomes.The inconsistent results of clinical trials may have related to aspects of drug composition,dosing,delivery,and timing.At this time,surfactant therapy cannot be recommended as routine therapy in pediatric ARDS.

After the basic contents of MOOC and flipped classroom were introduced, MOOC-based flipped classroom teaching model for Chinese medical literature information retrieval course was described, its design and implementation were elaborated with CBM Database as an example.

OBJECTIVE:To prepare Ankang oral liquid and to establish its quality control method.METHODS:Quali?tation identification of the principal agents like Fructus citri sarcodactylis and Radix Curcumae were performed by TLC,the content of hesperidin was detemined by HPLC.RESULTS:The TLC spots were clear and centralized,the linear range of hesperidin was0.04168?g～0.4168?g,the average recovery was98.11%,RSD=0.25%.CONCLUSION:The preparing technique was simple and its quality is stable,the quality control method is accurate and feasible.

The abnormalities of retinal vessels such as retinal arteriolar narrowing,arteriovenous nicking,micro-aneurysms,retinal hemorrhages,and cotton wool spot are closely related to systemic diseases including hypertension,diabetes mellitus,cardiovascular disorders,stroke and renal diseases.The modern retinal vessels examination technology has features of quick noninvasive,quantitative standardized and intelligent analysis.Taking advantage of these to fully discover the retinal vascular abnormalities and get deeper understanding of the relationship between its' mechanism and systemic vascular diseases is not only helpful to better diagnose and treat retinal vascular diseases,but also contributes to predicting the risk and prognosis of systemic diseases.We suggest emphasizing on the study of correlation between retinal vascular abnormalities and systemic vascular diseases using modern retinal vessels examination technology.It will provide the preventive clue of diseases of circulatory system by finding out the retinopathy.Meanwhile,correctly treating systemic diseases would get a better prognosis of the retinopathy.They exist side by side and play a part together for providing a better prognosis,which would be a new direction for the doctors and scientists in the new era.

The epidermal stem cell was introduced briefly in this review.And then the spacial characteristics of diabetic wound healing were summarized.In this regards,we focus on the important role of epidermal stem cell in diabetic wound healing.At last,we outlook the research direction and clinical application of epidermal stem cell in diabetic wound.

Objective To establish the determination method of gallic acid in Yuanhu Zhitong Soft Capsule by HPLC. Methods Chromatography was performed on a Unitary C18 column (250 mm× 4.6 mm, 5 μm) at 254 nm. The mobile phase consisted of a mixture of methanol and 0.5%phosphate acid (5∶95) at flow rate of 1.0 mL/min. Results The standard curve of linear relationship was 0.024 8-0.496 μg and r=0.999 9. The RSD of precision, repeatability and stability was 0.27%, 0.21%, 1.40%, respectively. The average recovery was 99.81%, RSD was 0.72%. Conclusion The established method was accurate and reliable for the determination of gallic acid in Yuanhu Zhitong Soft Capsule.

Induced pluripotent stem cell (iPSC) is a type of pluripotent stem cell that can be generated directly from adult cells through gene reprogramme and cell dedifferentiations.The researching history and advantages of iPSC were reviewed in this paper.In addition,the application of iPSC on diabetis mellitus was also summarized and prospected.