Objective:To investigate the relationship among serum uric acid,C-reactive protein and the degree of carotid artery atherosclerosis in patients with essential hypetension.Methods:122 patients with essential hypertension were divided into intima-media thickness group(Ⅰ),plaque development group(Ⅱ) and vessel stenosis group(Ⅲ).Concentration of serum uric acid and C-reactive protein were measured in each patient and the relationship between serum uric acid and C-reactive protein was observed.Results:The concentrations of serum uric acid and C-reactive protein in group Ⅱ and Ⅲ were positively connected with pathologic changes of carotid artery atherosclerosis( P

(0.05)).Conclusions PE is an effective form of treatment for recurrent FSGS by removing circulating factor,especially if initiated early.Failure to achieve remissions and maintain stable remission after the initial period of PE imply a poor outcome and glomeruli lesion been sclerosis.Therefore,PE should be performed as early as possible until completely remissions.

Objective:To investigate the effects of trimetazidine on cardiac systolic and diastolic function in patients with chronic congestive heart failure due to coronary artery disease.Methods:45 patients with chronic congestive heart failure due to coronary artery disease were divided into two groups,23 patients in trimetazidine group and 22 in placebo-control group.The echocardiogram indices of cardiac function of patients in two groups-SV,CO,CI,LVEF,VA,VE and VA/VE were measured before and after treatment.Results:The indexes SV,CO,CI and LVEF were significantly higher in trimetazidine group than those in control group(P0.05).Conclusion:Trimetazidine can improve systolic function of ischemic heart by improving the energy metabolism of ischemic heart.

Objective To investigate the impact of different surgical methods and different doses of euthyrox on bone mineral density.Methods A sample of 40 patients received thyroid cancer operation (excised affected side leaf,aisthmus,most of offside and neck VI lymph node dissection)and 40 patients received nodular goiter operation (excised affected side leaf)were collected,the dose of euthyrox taken in was 85 .2μg in thyroid cancer patients and 36.2μg in nodular goiter patients daily,the bone mineral density 6 month and 1 year after operation was detected. Results There was no difference of BMD between the two group 6 month after operation (P>0.05),but BMD of the observation group was (0.64 ±0.28)g/m2,which was lower than that of control group (t=2.235,P=0.03).Com-pared with pre-operation,BMD of control group had no change and the observation group appeared drop 1 year after operation.Conclusion Patients received thyroid cancer taking in euthyrox may show BMD reduced,especial 1 year after operation,doctors should be on the alert and supplement calcium appropriately.

A lot of children with congenital heart disease have an abnormal development of nervous system. A variety of biochemical and physical examination indices can be used to predict adverse outcome of neurodevelopment caused by congenital heart disease and they contribute to early intervention and improvement of neurodevelopmental disorders. In recent years, new progresses in the laboratory tests, neurological physiccal examination and perioperative management of the neurodevelopmental abnormalities caused by congenital heart disease have been made. The predictive factors of abnormal neurodevelopment caused by congenital heart disease were reviewed in this article.

AIM: The aim of the present study was to clarify the mechanism of intracellular signal transduction in Ang Ⅱ- induced proliferation of vascular smooth muscular cells (VSMC) by observing the effect of c- Src on Ang Ⅱ- mediated mitogen- activated protein kinase (MAPK) activation and c- Fos protein expression in cultured VSMC of rats. METHODS: Cultured aortic VSMCs from SD rats were transfected with anti-sense c-Src oligodeoxynucleotides (ODNs) wrapt with lipofectin to inhibit c- Src activity and protein production. Untransfected VSMCs were used as control. We observed the role of Ang Ⅱ stimulation in MAPK activation and c- Fos protein expression. c- Src kinase activity was measured by protein immunoprecipitation and kinase autophosphorylation. The phosphorylation rate of the substrate myelin basic protein (MBP) was employed to assess MAPK activity.Western immunoblot was used to detect protein expression of c- Src and c-Fos. RESULTS: c-Src protein expression in VSMC transfected with different concentrations of anti- sense ODNs significantly decreased in a negative dose- effect manner. c- Src kinase activity was also markedly inhibited. Following the stimulation of Ang Ⅱ on transfected VSMCs with anti-sense ODNs, the increase rate of c- Src activity was 8.7% of that in control, the activity of MAPK was 1.6% compared with control and c- Fos protein expression was as 30.0% as that of control. CONCLUSION: Ang Ⅱ induces c- Src activation. MAPK activation and c - Fos protein expression by Ang Ⅱ is dependent on c- Src activation. These findings indicate that c- Src is an important signal factor in Ang Ⅱ-induced VSMC proliferation.

Objective To investigate the mechanism of apoptosis induced by endoplasmic reticulum stress during sepsis.Methods Septic model of H9C2 cells was established by lipopolysaccharide (LPS).CHOP mRNA,mitochondrial membrane potential (MMP) and apoptosis were assessed by real-time PCR,flow cytometry respectively at LPS administration 24 h with or without salubrinal (inhibitor of endoplasmic reticulum stress).Results The expression of CHOP mRNA and apoptosis were upregulated,but MMP was decreased after LPS exposure 24 h compared with control group[2.40 ±0.30 vs 1.00 ±0,(16.58 ±2.71) ％ vs (3.85 ± 1.07) ％,1.07 ± 0.33 vs 2.62 ± 0.51,P ＜ 0.05).Compared with LPS group,salburinal pretreatment decreased LPS-induced upregulation of CHOP mRNA (1.60 ± 0.23 vs 2.40 ± 0.30,P ＜ 0.05),meanwhile,salburianl alleviated MMP(1.85 ± 0.31 vs 1.07 ± 0.33,P ＜ 0.05) and LPS-induced apoptosis [(6.05 ± 1.48) ％ vs (16.58 ± 2.71) ％,P ＜ 0.05].Conclusion Salubrinal plays a cytoprotective role against apoptosis induced by LPS through inhibition of CHOP mRNA,which leads to mitochondrial impairment.Endoplasmic reticulum stress may induce apoptosis via mitochondrial pathway in the septic model of H9C2 cardiomyocytes.

Medical electron linear accelerator and Gamma-ray stereotactic radiotherapy system,two kinds of large scale medical equipment,both have an medical treatment on patient by imposing a high-level of radiation.The accuracy of deferent dose and validity of therapy programe play a very important role to patients'security and treatment effect.Both the military and local governments Pay a lot of attention to the two kinds'allocation and quality control.As technical administrative department.the Medical Equipment Application Quality Test and Research Center of PLA analyse current situation of the two kinds detailedly and point out the existing problems based on quality of the two kinds of equipment used in military medical agencys.

Objective To investigate the mechanism of liver X receptor(LXR)signal pathway in regulating glucose metabolism by observing the variety of LXR expression and its impacts on regulating the mRNA expression of PEPCK and GCK, the key enzyme of hepatic glucose metabolism in various glucose metabolic status in rats. Methods SD rats were chosen and divided into four groups:the CON group, the induced DM group, the OB group, and the induced OB+DM group. For each group of rats, body weight, blood glucose, serum triglycerides, and cholesterol were measured. Then the rats were sacrificed and the livers were collected and studied. Real-time PGR was used to measure the expressions of LXR mRNA, PEPCK mRNA, and GCK mRNA in the livers. Finally, the Western Blot assay was used to measure the liver LXR protein expression. Results The expression of LXR mRNA was significantly higher in DM,OB, and OB+DM groups than in CON group(P＜0.05).The Western blot results showed that the levels of protein were in accordance with the mRNA expression. Comparing to the CON and the OB groups, the PEPCK mRNA expression of the OB+DM and the DM groups was significantly higher, while the GCK mRNA expression of these two groups was significantly lower(P＜0.05). Comparing to the CON group, the PEPCK mRNA expression of the OB group was significantly lower, while the GCK mRNA expression of OB group was significantly higher(P＜0.05).Conclusions During non-diabetic phase, LXR could act as a protective receptor for glucose metabolism and keep glucose homeostasis by regulating the key enzymes of the hepatic glucose metabolism. While in the diabetic phase, the protective receptor LXR failed to reverse the change of the related enzymes caused by insulin deficiency, and finally the plasma glucose level was raised.