AIM: To extract Zanthoxylum oil from the seeds of Zanthoxylum bungeanum Maxin, and find out a better extraction method and its optimized operating conditions. METHODS: Zanthoxylum oil was extracted by distillation method, solvent extraction and supercritical fluid extraction, respectively. The products gained in each experiment was analyzed by GC after it had been methyl esterified. RESULTS: The yield of Zanthoxylum oil extracted by distillation, solvent extraction and supercritical fluid extraction was 0.88 %, 13.73 % and 13.52 %, respectively, and its content of unsaturated fatty acid 4.50 %, 65.97 %, 74.97 %, respectively. CONCLUSION: Supercritical fluid extraction was the better of the three mehtods, whose optimized operating conditions consisted of 30 MPa pressure, 50 ?C operating temperature and 5 h extraction time.

Objective To study the sleep quality of the parents whose children with cerebral palsy (CP).Methods The Pittsburgh sleep quality index (PSQI) as an investigative tool was used to investigate 36 cases of parents with CP children and 36 cases of parents with normal children.Results The sleep problem report rate was 34.72％ in parents with CP children,and 19.44％ in parents with normal children,their difference was statistically significant (x2 =4.255,P ＜0.05).Score of PSQI(5.72 ±3.54 vs 3.19±2.76,t =3.380,P ＜0.01),sleep quality(1.33 ±0.83 vs 0.78 ±0.34,t =3.371,P ＜0.01),fall asleep time (1.28 ± 0.88 vs 0.72 ± 0.36,t =3.027,P ＜ 0.01),hours of sleep (1.16 ± 0.72 vs 1.01 ±0.62,t =2.278,P ＜ 0.05),and insomnia(1.23 ± 0.56 vs 0.75 ± 0.28,t =2.949,P ＜ 0.01) of parents with CP children were significantly different from the parents with normal children.Score of PSQI (6.21±0.85 vs4.32 ±0.73,t =3.380,P ＜0.01),sleep quality(1.14 ±0.73 vs 0.89 ±0.66,t =2.986,P＜ 0.01),fall asleep time (1.22 ± 0.81 vs 0.96 ± 0.83,t =2.853,P ＜ 0.01),hours of sleep (1.09 ± 0.66vs 0.85 ± 0.71,t =2.136,P ＜ 0.05),insomnia (1.15 ± 0.63 vs 0.83 ± 0.62,t =2.513,P ＜ 0.01)and daytime function(1.19 ± 0.43 vs 0.88 ± 0.62,t =2.586,P ＜ 0.01) of mothers with CP children were significantly different from fathers with CP children.Conclusions The sleep quality of parents of CP children are worthy of attention.

BACKGROUND:Studies have shown that genetical y modified Schwann cel s can survive for a longer time in vivo, and promote nerve regeneration and functional recovery. OBJECTIVE:To transfect human telomerase reverse transcriptase (hTERT) gene into rat Schwann cel s cultured in vitro via PLXSN vector, and to detect the telomerase activity and biological characteristics of Schwann cel s. METHODS:Schwann cel s from Wistar rats were cultured in vitro and transfected by PLXSN vector with (hTERT group) or without hTERT (empty vector group). Normal Schwann cel s were selected as control group. RT-PCR and western blot methods were used to detect the hTERT protein and mRNA levels in Schwann cel s, and flow cytometry was used to measure the cel cycle distribution. Cel growth was observed by cel growth curve and MTT colorimetric method. RESULTS AND CONCLUSION:At 48 hours after transfection, the mRNA and protein expressions of hTERT were remarkably seen in Schwann cel s. Compared with the control and empty vector groups, the cel s grew faster, the number of cel s at G 0/G 1 Schwann cel s cultured in vitro. phase was reduced, but the number of S phase cel s was increased in the hTERT group (P<0.05). These findings indicate that PLXSN vector-mediated hTERT transfection of Schwann cel s can significantly improve the activity of telomerase in Schwann cel s as wel as promote the proliferation of Schwann cells cultured in vitro.

OBJECTIVE To investigate probationer nurses' knowledge about nosocomial infection (NI) and clean operating-room. METHODS Survey among probationer nurses was performed by self-designed questionnaires. RESULTS 47% probationer nurses thought it very necessary to strength knowledge about NI before probation. The scores of clean operating-room knowledge was lower than that nosocomial infection (NI); The cognition rate among high educated probationer nurses were higher than low educated,P

ObjectiveTo observe the clinical efficacy of acupuncture plus TDP (specific electromagnetic treatment apparatus) and auricular point sticking in treating chronic pelvic infection.MethodSixty patients diagnosed with chronic pelvic infection were randomized into two groups. Thirty cases in the treatment group were intervened by acupuncture plus TDP and auricular point sticking, while the other 30 in the control group were by acupuncture plus TDP, 10 d as a treatment course in both groups, and the therapeutic efficacies were compared after 3 courses.ResultThe total effective rate was 96.7% in the treatment group versus 83.3% in the control group, and the difference was statistically significant (P<0.05).ConclusionAcupuncture plus TDP and auricular point sticking can produce a marked therapeutic efficacy in treating chronic pelvic infection, and its total effective rate is higher than that of acupuncture plus TDP.

AIM: To set up identification of Flos Lonicerae and determination of chlorogenic acid in Yingqiao jiedu Pills (Flos Lonicerae, Fructus Forsythiae, Radix Glycyrrhizae, Herba Menthae, etc.) by HPLC. METHODS: A YWG C 18 column was used with the mobile phase consisted of CH 3OH 10%Na 2HPO 4 (adjusting pH to 2.7 with HAc) as gradient elution. The flow rate was 1.0mL?min -1 , and the detection wavelength was at 326nm. RESULTS: Flos Lonicerae in Yinqiaojiedu Pills was identified by HPLC UV. The linear range of chlorogenic acid was 5.0～60?g?mL -1 ( r =0.9999). The average recovery was 98.71% with RSD of 1.55% ( n =5). CONCLUSION: The method is simple, accurate and rapid with good reproducibility. It can be used for the quality control of Flos Lonicerae in Yinqiaojiedu Pills.

OBJECTIVE To analyze the risk factors and antimicrobial resistance pattern of Stenotrophomonas maltophilia isolated from 6 hospitals in Beijing,and study the difference in resistance rate of isolates from different hospitals. METHODS In this study,145 cases with S.maltophilia infection were analyzed and their susceptibility was tested.The synergetic screening test was applied to detect metallo-?-lactamases. RESULTS Most of 145 strains were isolated from sputum(86.2%),mainly from ICU(48.3%),and department of respiratory medicine(22.1%).The drug sensitivity tests in vitro showed these strains were resistant to commonly used antibiotics,and drugs whose sensitive rate was over 50% included doxycycline,gatifloxacin,cefoperazone-sulbactam,levofloxacin,compound sulfamethoxazole,ceftazidime-clavulanate and ticarcillin-clavulanate.The antimicrobial resistance of strains isolated from different wards showed some difference.Of all the infected patients,56.7% had underlying diseases;92.2%were treated previously with broad-spectum antibiotics;76.8% underwent invasive examination or treatment. CONCLUSIONS S.maltophilia is resistant to many kinds of antibiotics.The infection caused by S.maltophilia often occurs in patients with severe underlying disease and low immunity.Antibiotics should by chosen by drug sensitivity tests.

Teaching is very important for anesthesiology developmemt,so residents should be cultivated the teaching capacity as soon as possible.This article discussed how to cultivate the teaching capacity of residents in anesthesiology department.

Objective To summarize the data of newborn screening in Yongchuan district of Chongqing to promote its quali‐ty ,and to provide some measurements to enhance the management of newborn screening in this area .Methods According to the management system and technical standards of newborn screening (2010 Edition) ,newborn screening in Yongchuan district was im‐plemented .Screening rate ,coverage rate of newborn screening in maternity hospital ,recall rate of suspiciously affected newborns and rate of treatment of patients were analyzed from 2013-2014 .Results The screening rate ,coverage rate of newborn screening in maternity hospital ,recall rate of suspiciously affected newborns were increased by 77 .32% ,88 .57% and 17 .44% ,respectively , during these 10 years .All patients were treated appropriately (100% ) .Congenital hypothyroidism and phenylketonuria were screened .Since 2010 ,glucose‐6‐phosphate dehydrogenase deficiency and congenital adrenal hyperplasia were added to the newborn screening test panel .Conclusion Strengthen the management ,establish administrative department ,faculty training ,health education and government financial supports are contributed to improving the quality of newborn screening .

Objective To argue whether the Chinese version of Emotional Intelligence Scale (EIS) can be used to evaluate emotional intelligence of nursing students through studying its reliability and validity,and provide basis for the study of emotional intelligence of nursing students.Methods Using stratified random sampling method,Chinese version of EIS compiled by Wang Caikang was used to carry out the questionnaire survey among university and college students of different nursing specialty,using Cronbach coefficient alpha and factor analysis method to study the reliability and validity.Results Each dimension and total reliability of the Chinese version of EIS were 0.735,0.815,0.801,0.790,0.821.Through the principal component analysis,four common factors were extracted,the cumulated variance contribution of the four factors was 55.468％.Conclusions Chinese version of EIS has good reliability and validity,which is fully applicable to nursing students' emotional intelligence research.