To test the apoptosis inducing effect of hypoxia on cardio myocytes Neonatal Wistar rat cardiomyocaytes were cultured under hypoxic condition for 2 hours,12 hours,24 hours,respectively Flow cytometery,TUNEL and morphological method were used to detect the apoptotic myocytes We found that the number of apoptotic cells of each hypoxia group was greater than that of the control group,and it increased as the time of hypoxia went longer The apoptotic cell number of the control group and the hypoxic groups of 2nd,12th and 24th h. were 9 33?0 62%,17 29?0 95%,33 59?2 09% and 76 01?4 60%( P

A novel polymer- overoxidized polypyrrole film chemically modified electrode (CME) as the liquid chromatography-electrochemical detector (LC-ECD) was fabricated and applicated, which could be used to determine the monoamine neurotransmitters. The experimental model of parkinsonian animal was established by using medicine. Microdialysis sampling coupled with LC-ECD was used to monitor the monoamine neurotransmitters and their metabolites in experimental animal brain in vivo under different situations. The mechanism of experimental animal's Parkinsonian disease was studied elementarily. An accurate and reliable analysis method was supplied for filting the new and the more effective medicine to cure parkinsonian disease.

Objectives: To observe the effect of total enteral nutrition (TEN)and total parenteral nutrition (TPN)in aged patients with mechanical ventil ation. Methods: 60 cases of aged patients with mechanical ventilation were randomly divided to TEN group and TPN group.All patients accepted the same amount of calorie and nitrogen. Results: with regard to albumin and hemoglobin levels and nitrogen equilibrium TEN group was superior to parenteral nutrition group ( P 0.05). Conclusions: Enteral nutrition can reduce mechanical ventilation time and lower treatment cost in aged patients.

Objective To investigate blood uric acid levels in health check-up receivers in Tianjin City. Methods Blood uric acid levels in 28 661 health check-up participants were tested and the incidence of hyperuricaemia was analyzed. Blood pressure, body mass index, blood sugar, cholesterol and triglyceride were measured and compared. Results The average level of blood uric acid was (349±73 ) μ mol/L in male,and (256±59) μmol/L in female. The peak value in male was found in the 30 to 39 years old group,while in female the age was increased to 70 years old. The incidence of hyperuricaemia was higher in male ( 15. 2% vs 6. 3% ,P＜0.01). The level of blood sugar,triglycerides and total cholesterol was higher in the hyperuricaemia group. Also in the hyperuricaemia group, the incidence of overweight/obesity,hyperglycaemia, hyperlipidemia and hypertension was higher. Conclusions The incidence of hyperuricaemia in routine health check-up individuals in Tianjin City could be 11.3%. Blood uric acid may be significantly associated with metabolism.

Objective To investigate the characteristics of cerebral vascular imaging and risk factors of stroke in popu?lation with family history of stroke in rural areas of Ji County, Tianjin. Methods The volunteers with family history of stroke were recruited in the study to undergo computed tomography and computed tomography angiography (CTA) of head as well as physical and laboratory examinations. A total of 281 subjects, including 153 male and 128 female, underwent exami?nation, and were divided into stroke group and non-stroke group. Results The detection rates were significantly higher in male subjects than those of female subjects, including smoking rate, drinking rate, educational level, and decreased level of high density lipoprotein cholesterol (HDL-C), whereas the detection rate of high level of triacylglycerol (TG) was significant?ly higher in female than that of male subjects (P<0.05). There were 38 subjects in stroke group. The age, internal carotid ar?tery calcification rate (ICAC) and internal carotid artery tortuosity (ICAT) rate were significantly higher in stroke group than those of non-stroke group (P<0.05) . The results of multivariate Logistic regression analysis indicated that age, hyperten?sion, ICAC, and ICAT were independent risk factors for stroke. Conclusion For population with family history of stroke, age, hypertension, ICAC and ICAT were risk factors of stroke.

Objective:To investigate the correlation between anthropometric indices and arteriosclerosis detection indicators in the middle-aged and the elderly .Methods: A cross-sectional descriptive study was made of 1 626 individuals ( diabetics patients 23.37%, hypertensive subjects 39.48% and healthy individuals 37.15%), aged 45 to 90 years [mean age:(61.60 ±10.22) years)] in Shijingshan Dis-trict, Beijing.Their measurements:body mass index (BMI), waist circumference (WC), and waist to height ratio ( WHtR ) .Arterial stiffness was assessed according to brachial-ankle pulse wave velocity (baPWV), intima-media thickness (IMT), augmentation index (AI) and ankle-brachial index (ABI), which were measured by noninvasive detectors and equipment .The correlations between the various indi-cators were analyzed .Results:The percentages of the hypertensive and diabetic groups of central obesity ( male WC>85 cm, female WC >80 cm or WHtR>0.5 ) and of general obesity ( BMI>28 kg/m2 ) were significantly higher than those of the healthy group ( P<0 .01 ) .The difference between the two disease groups was not significant ( P>0 .05 ) .There was a moderate positive correlation between the measures of abdominal obesity (WHtR and WC) and the general obesity indicators (BMI) (r=0.710 and 0.716) .In the healthy group , WC and WHtR showed positive correlation with baPWV , IMT and ABI, and negative correlation with AI 75 .BMI showed positive correlation with IMT and negative correla-tion with AI75 , and no correlation with baPWV and ABI .There was negative correlation between BMI and baPWV in the diabetic group .In the hypertension group , we found negative correlation between BMI and baPWV, maximum IMT, AI75 , and also between WC and AI 75 .The simple regression straight line of baPWV versus the anthropometric parameters showed that the regression equations were y =0 .949 +1.379 x (baPWV vs.WHtR, R2 =0.046, P<0.001) and y=1.133+0.006x (baPWV vs.WC, R2 =0.027, P<0.001), respectively.baPWV and BMI did not have a linear relationship (P =0.62). Conclusion:WHtR and WC are superior to BMI indices in predicting arteriosclerosis .Anthropometric measurements for central obesity are good predictors of cardiovascular risk .

Objective Using manganese enhanced MR imaging(ME-MRI)as a standard to validate the accuracy of white matter diffusion tenor tractography(DTT)with different gradients orientations in macaque monkeys.Methods Seven adult male macaque monkeys were scanned for DTY images with different number of gradients orientations.When all data were acquired.3 μl of 0.8 mol aqueous solution of MnCl2 was injected into the primary motor cortex representing forelimb for ME-MRI scanning 48 hours later.On the images of fractional anisotropy(FA),seed region for fiber tracking was chosen according to the subcortical enhanced range on ME-MRI,then the DTT fiber tracts proiecting on axial images of FA were compared with enhancement region on T1WI of ME-MRI.Results Under the calculation method for fiber tracking in the study,different motion-probing gradients orientations led to results consistent with ME-MRI for the brain above the midbrain level.All group's concordance score of fibertracking results were good.Among them,30 and 64 orientation fiber tracking results were optimal.Below the midbrain level,there was significant difference between DTT fiber tracking and ME-MRI fiber tracking. Conclusion Different motion-probing gradients orientations do not influence DTT fiber tracking.But below the midbrain level.the results of DTT fiber tracking were not reliable according to the standard results of ME-MRI fibe tracking.

Objective To assess the effect of combined hypertension (HT) and aging on left atrial (LA) size and phasic function.Methods This evaluation was based on the data from a cross-sectional study including 738 subjects with high risk for cardiovascular disease from an urban community in Beijing.Subjects were divided into 3 groups according to age (41-59,60-69 and ≥70 years) and further into HT and non-HT sub-groups.LA volume index were calculated and LA global longitudinal strain in late diastole (Sa),early diastole (Se),and total strain (Stot =Sa + Se),and strain rate in late diastole (SRa),systole (SRs),and early diastole (SRe) were measured using off-line speckle-tracking echocardiography.Results LA volume index increased significantly in HT groups with aging,whereas no changes could be viewed in non-HT subjects among all age groups.LA conduit index (Se and SRe) decreased with aging in both HT and non-HT subjects with more sever in HT subjects than in non-HT subjects in all age groups.The LA conduit index in 41-59 year-HT,and in 60-69 year-HT subjects were comparable with that in 60-69 year-non-HT subjects [Se (11.0 ±4.4)％ vs (11.6 ±4.7)％,SRe (1.0 ±0.4) s-1 vs (1.0 ±-0.3) s-1],and in ≥70 year-non-HT subjects [Se(10.1 ±4.0)％ vs (9.5 ±5.4)％,SRe (0.9 ±0.3)s-1 vs (0.8 ± 0.4) s-1],respectively.LA reservoir (Stot and SRs) and contraction (Sa and SRa) index also decreased with aging in HT but not in non-HT subjects.Conclusions Aging along does not lead to LA enlargement in subjctes,but it does when combined HT.There is synergistic effect of HT and aging on LA volume and phasic function.

Background To report quality control methods and baseline reproducibility data of the ultrasound measurements of carotid artery intima-media thickness in the project of Establishment of an Integrated System for Coronary Heart Disease Prevention and Treatment.Methods Standard ultrasound scanning and measuring protocols were established by the study group.All sonographers and readers were trained by the carotid ultrasound core lab and all digital ultrasound images were centrally read.Ten subjects were scanned twice (with 1 week interval) by 2 sonographers independently and images were read by a single reader to evaluate the sonographer variability.Twenty subjects' images were read twice (with 1 week interval) by a single reader to assess the reader variability and the reproducibility of IMT measured at different carotid segments.Results The intraclass correlation (ICC) of intra-and inter-sonographer and intrareader for mean IMT measurements was 0.99,0.98 and 0.97 respectively; while for max IMT,it was 0.97,0.99 and 0.95 respectively.Among different carotid segments and sites,ICC for mean IMT measurements of common carotid (CCA),carotid artery bulb (Bulb),internal carotid artery (ICA),overall near wall and overall far wall was 0.97,0.99,0.89,0.93 and 0.98 respectively.Conclusion The reproducibility of IMT measurements according to our protocol is acceptable,although better reproducibility is found when measuring the mean IMT than max IMT,CCA and Bulb IMT than ICA IMT,and far wall IMT than near wall IMT.

Objective To explore the prevalence and clinical and echocardiography features of the basal septal hypertrophy(BSH).Methods Clinical and echocardiography data of 1 056 elderly population in an urban community of Beijing were analyzed.BSH was defined as the thickness of basal interventricular septum ≥1.4 cm and basal septal/mid septal ≥1.3.Data were compared between BSH and non-BSH,and the risk factors of BSH were evaluated.Results The prevalence of BSH in this population was 7.39%(95%CI:5.8%-9.0%).BSH was not associated with current cardiovascular diseases (P >0.05).Its correlates in logistic analysis included male,diabetes mellitus,small end diastolic left ventricular dimension and abnormal left ventricular diastolic function (P <0.05),with OR 0.49(0.29,0.83),1.99(1.18,3.37),2.24 (1.35,3.72),0.39(0.26,0.59),and 1.96(1.01,3.81),respectively.Conclusions BSH is common in elderly community population and not associated with cardiovascular diseases.Its risk factors included male, obesity,diabetes mellitus,small end diastolic left ventricular dimension and abnormal left ventricular diastolic function.