Objective To deeply investigate the psychosocial factors affecting Chinese nurse occupational burnout.Methods An online literature search was carried out in China National Knowledge Infrastructure,VIP Database for Chinese Technical Periodicals and Wanfang database to collect relevant articles.CMA2.0 was used to analyze the fifty literatures which satisfied the uniform inclusion and exclusion criteria.Results (1)On emotional exhaustion and depersonalization,passive coping styles had moderate positive effect,neuroticism had high positive effect,work stress had low positive effect; active coping styles,self-efficiency,social support and extroversion had low negative effect.Psychotics and lying had positive or negative moderate effect on depersonalization respectively.(2)On personal accomplishment,self-efficiency had high positive effect,active coping styles,lying and subjective support had moderate positive effect,neuroticism,psychotics and work stress had small negative effect,passive coping styles had no effect.(3)Self-esteem had no significant relationship with occupational burnout.Conclusions Nurse's occupational burnout resulted from psychosocial factors,including coping style,self-efficiency,personality,social support and work stress.

B7-H4 is a new member of costimulatory molecules,it negatively regulates T cell immunity by the inhibition of T cell proliferation, cytokine production, and cell cycle progression. Recent studies indicate that B7- H4 is associated with the generation and progression of tumor and is an important indication in the diag-nosis and treatment of tumor.

In recent years,more and more attention has been paid to the inflammatory reaction in the process of neonatal unconjugated bilirubin brain damage.Studies have shown that inflammation plays an important role in the occurrence and prognosis of neonatal bilirubin brain injury.A large amount of unconjugated bilirubin pass through the blood brain barrier,causing glial ceils activation,emerging immune response,releasing inflammatory cytokines,leading the apoptosis,necrosis and damage repair of neuronal.This article reviews the occurrence and protection mechanism of inflammatory cytokines(tumor necrosis factor-α,interleukin-6,8,1 β,10,et.al) in neonatal bilirubin brain damage.

Objective:To explore the shortages of chemotherapy occupational protection for pharmacist in our hospital through the contrast and analysis of the occupation protection status of pharmacists in two groups.Methods:Chemotherapy Occupational Protection Status Questionnaire for Medical Staff was used,and totally 30 pharmacists from PIVAS and 30 ones from OUIVA were selected as the research objects according to the random number table.The main contents of the questionnaire included the basic information of respondents,protection conditions in drug preparation rooms,pharmacist protection status and pharmacist training conditions,etc.The results of the questionnaire were statistically analyzed.Results:In general,the understanding of pharmacists for the harm of chemotherapy drugs and protective measures was poor.PIVAS group was obviously better than OUIVA group in the use of protective equipment and relavant measures with statistically significant differences (P<0.05).Conclusion:Pharmacists have insufficient understanding for the danger of chemotherapy drugs and their harmful ways with low performance of chemotherapy occupational protection,and our hospital has no perfect protection system and adequate protective equipment yet.

Objective To develop and verify a reversed phase high-performance liquid chromatography method for the determination of the purity of recombinant Mycobacterium tuberculosis(Mtb)Ag85b protein stock solution.Methods Fourfactor,three-level orthogonal test was designed,with the area,trailing factor,peak area and peak area RSD as the evaluation indexes to explore the optimal detection conditions. The methodology verification of specificity,linear range,precision and durability was conducted in accordance with the general principles of Chinese Pharmacopoeia(Volume Ⅳ,2020 edition)9101.Results The results of all the evaluation indexes were good when the elution ratio of organic phase was30% ～ 95%,the detection temperature was 35 ℃,the sample volume was 3 μg,and the elution time of 95% organic phase was 15 min. The method had the linear correlation coefficient(R2)of 0. 998 5,the linear range of 1. 8 ～ 4. 2 μg,the reproducibility RSD of 0. 01%,and the intermediate precision RSD of 0. 16%,with good durability under slight changes of column temperature and flow rate.Conclusion The reversed phase high-performance liquid chromatography method for the purity determination of recombinant Mtb Ag85b protein stock solution was developed,which has good specificity,precision and durability,and can be used for the quality control of recombinant Mtb Ag85b protein stock solution.

Objective:To detect changes in mitochondrial membrane potential after sperm mother cell damage induced by bisphenol A by laser scanning confocal microscopy(LSCM) and underline its potential action mechanism.Methods: The cultured spermatogenic cells were divided into 3 groups, respectively. Then 0, 10μmol/L, 100μmol/L of Bisphenol A(BPA) were added into the culture, after 3 hours culture, fluorescence probe JC-1 was used to lable the three groups. The fluorescence intensity of JC-1 in mitochondrial was then detected by LSCM. LSCM software was used to analyze the fluorescence intensity. Change of the mitochondrial membrane potential was represented by the change of fluorescence colors (relative proportion of red and green fluorescence is commonly used to measure mitochondrial depolarization ratio). Results:The ratio between the red and green fluorescence in the control group, low dose group and high dose group had significant difference. Intracellular mitochondrial membrane potential of Bisphenol A treatment group was lower than that of the control group, while mitochondrial membrane potential of high dose group was lower than that of the low dose group. Conclusion: Bisphenol A could damage spermatocytes, and as the dose increased, the more serious the injury. The method could real-time monitor with high sensitivity the change of intracellular mitochondrial membrane potential.

The rapid development of MOOC is both an opportunity and a challenge for the higher education in our country .The information literacy of college and university teachers plays an important role in the success implemen-tation of MOOC.The development and characteristics of MOOC were described, the problems existed in information literacy of college and university teachers under MOOC environment were analyzed with strategies put forward for improving the information literacy of college and university teachers from the aspects of ideological concept , training methods, assessment criteria, and information environment .

Objective To understand the situation of HIV and HCV infection of heroin dependent patients .Methods HIV and HCV serological detection were proceed among 565 heroin dependent patients ,and the results were compared according to genders and nationalities .Results Among 565 heroin dependent patients ,the infection rates of HIV ,HCV ,and HIV/HCV for males were 14 .57% (72/494) ,59 .72% (295/494) ,and 13 .36% (66/494) respectively ,and those for females were 19 .72% (14/71) ,77 .46%(55/71) and 16 .90% (12/71) respectively .The infection rates for males were significantly lower than females (P<0 .05) .There were significant difference of the infection rates of HIV ,HCV and HIV/HCV between Han patients and Uyghur patients(P<0 .05) .Conclusion The heroin dependent patients are high risk population for HIV and HCV infection .Education and publication of drugs and infectious should be enhanced in order to control the spread of disease and reduce disease incidence .

The incidence of severe acute pancreatitis (SAP) is increasing year by year,and the mortality is high,which poses a great threat to the life of patients.In recent years,the immune nutrition therapy has become the focus in the treatment of SAP,among which,fish oil lipid emulsion as a novel type of fat emulsion,has the multi-effects of anti-inflammatory,anti-oxidative,immunomodulatory,promoting signal transduction and protecting organ function apart from providing nutrition and regulating metabolism.It can prevent the deterioration of the whole body from systemic inflammatory response syndrome (SIRS) to multiple organ dysfunction syndrome (MODS and ultimately improve the prognosis of patients.