1.Protective Effect of Stichopus hermanii Against Oxidative Stress Induced by Chronic Cigarette Smoking in Rat Salivary Glands
Syamsulina Revianti ; Kristanti Parisihni ; Puguh Bayu Prabowo ; Lita Agustia
Malaysian Journal of Medicine and Health Sciences 2020;16(Supp 4, July):76-82
Introduction: Stichopus hermanii (SH), which contains various antioxidant agents, tends to protect oxidative stress
caused by chronic cigarette smoking (CCS). This study, therefore, aims to investigate the protective effect of SH supplementation against CCS-induced oxidative stress in rat salivary glands. Methods: A total of 30 male Wistar rats,
which were equally divided into the control (C), cigarette smoke (CS), and treatment (T) groups, were used to carry
out this research. In T group, 17 mg/kg BW of SH was administered for 90 days. Their salivary glands were removed
for oxidative stress marker analyses ie malondialdehyde (MDA) level, total antioxidant status (TAS), superoxide
dismutase (SOD) and catalase (CAT) activity, with the data analyzed using one-way ANOVA and Tukey’s multiple
comparison test to obtain a p-value of less than 0.05, which were considered statistically significant. Results: The results showed that in the CS group oxidative stress occurred which was characterized by significantly increased MDA
levels, reduced TAS, SOD and CAT activity. While the T group significantly decreased MDA levels, enhanced TAS,
SOD and CAT activity. Conclusion: In conclusion, SH supplementation tends to prevent oxidative stress produced
by CCS in rat salivary glands.
2.Innovative Treatment Using Salmon Skin Extract as Topical Application for Traumatic Oral Ulcer Healing: Animal Models
Syamsulina Revianti ; Puguh Bayu Prabowo ; Lita Agustia ; Isidora Karsini
Archives of Orofacial Sciences 2021;16(SUPP 1):37-42
ABSTRACT
Salmon skin extract contains high proline and hydroxyproline, and has been suggested as a potential
topical agent for traumatic oral ulcer healing. The aim of this study was to evaluate the effect of salmon
skin extract as traumatic oral ulcer healing. A total of 32 Wistar rats (200 g to 250 g) were distributed
into four groups. Group 1 served as the control group (no treatment), Group 2 was topically treated
with salmon skin extract agent 4%, Group 3 was topically treated with salmon skin extract agent 5%,
and Group 4 was topically treated with salmon skin extract agent 6%. Traumatic ulcers at lip mucosa
were performed in all rats and 0.1 ml salmon skin extract was applied on the ulcer twice daily for seven
days. The animals were euthanised on the last day of treatment. Biopsy specimens were taken from
the lip mucosa in all rats for epithelial thickness evaluation and the study for number of fibroblasts by
histological analysis. Significant increase in epithelial thickness and the number of fibroblasts (p > 0.05)
was observed in salmon skin extract treatment groups as compared to the control group. Salmon skin
extract 6% treatment group had the highest epithelial thickness and the number of fibroblasts amongst
the study groups. Salmon skin extract promises an innovative topical application treatment for traumatic
oral ulcer healing. Salmon skin extract 6% was the most effective concentration for traumatic oral ulcer
healing.
Epithelial Cells
;
Fibroblasts
;
Oral Ulcer--therapy