Only by meeting the demand of health reformation and development in medical high education can medical students settle the structural contradiction of employment. And only by keeping up with the employment tendency can they be easily employed.

Purpose:To investigate the telomerase activity in human digestive tract cancer and its significance and evaluate usefulness of the TRAP ELISA method .Methods:Telomerase activity was examined in 112 tumor specimens, including the following cancers: esophageal(12), gastric(36), hepatic(15), pancreatic (11), colorectal(38) and paracancerous tissues(94) by TRAP ELISA method.Results:The telomerase activity in tumor tissues was significantly higher than in paracancerous and normal tissues ( P

Objective To compare the risk factors of mild hemorrhage and diiferences of TOAST type in patients with acute cerebral infarction (ACI) and transient ischemia attack (TIA).Methods Forty patients with mild hemorrhage were divided into ACI group(24 cases) and TIA group(16 cases) according to disease classification.Risk factors such as age,blood pressure,blood lipid and so on between 2 groups were compared,TOAST subtype differences of 2 groups were compared.Results The age [(73.3 ± 4.1) years],the ratio of hypertension [95.8％(23/24)],leukoaraiosis [70.8％(17/24)] in ACI group was significantly higher than that in TIA group [(65.5 ± 5.6) years,13/16,9/16],there was significant difference (P ＜ 0.05).The ratio of large-artery atherosclerosis and cardioembolism in ACI group [75.0％ (18/24) and 16.7％ (4/24)] was significantly higher than that in TIA group (8/16 and 1/16),there was significant difference (P ＜ 0.05).But the ratio of small artery occlusion in ACI group [8.3％ (2/24)] was significantly lower than that in TIA group (7/16),there was significant difference (P ＜ 0.05).Conclusion Correlative risk factors of milde hemorrhage in ACI and TIA patients have significant differences.

Purpose:To investigate the clinicopathological characristics of colorectal mucinous adenocarcinoma (MUC),which is generally bel ieved to have a poor prognosis,in an attempt to assess ways in which the surgica l outcome can be improved. Methods:From 1994 to 2003,1 715 large intestinal cancer patient s underwent operation, among them, 197 patients were with MUC. The clinicopathol ogical parameters and prognosis of MUC and non-MUC were analyzed retrospectivel y. Results:There were no significant differences in sex , tumor si te and size. Patients with MUC had higher rate of serosal invasion (MUC:59.9% vs non-MUC: 35.1%) , invasive type lymph-node involvement (MUC:41.6% vs non-MUC 21.6%). Patients with MUC were of more advanced stage(D stage:MUC :44.1% vs non-MUC: 22.1%). Conclusions:Based on these findings , a more aggressive attitud e toward surgical intervention is recommended , including extensive lymph node d issection and the resection of adjacent organs that seems to be affected microsc opically , to improve the surgical outcome of this clinical entity.

ObjectiveTo investigate the prevalence of 15 skin diseases in adolescents from Liangshan prefecture in Sichuan Province.Methods A stratified cluster random sampling method was used to select subjects.Adolescents from 2 urban and 3 rural middle schools were investigated,who were required to fulfill self-report questionnaires and received physical examinations by doctors.ResultsA total of 7747 adolescents aged from 12 to 20(15.86 ± 1.86) years were included in the investigation,and skin diseases were present in 6379 adolescents,with a total incidence of 82.34％.In average,each subject suffered from 1.3372 ± 0.96861 skin diseases.Of the adolescents,4698(60.64％) had acne,2219(28.64％) freckle,966(12.47％) lichen pilaris,792(10.22％) eczema or dermatitis,511(6.60％) urticaria,195(2.52％) papular urticaria,37 (0.48％) vitiligo,55 (0.71％) psoriasis,166 (2.14％) verruca vulgaris,168 (2.17％) flat wart,317 (4.09％) tinea manus and pedis,142 (1.83％) tinea corporis,62 (0.80％) chloasma,26 (0.34％) nevus flammeus,15 (0.19％) nevus of ota.Male adolescents showed a higher incidence of acne(63.91％ vs.58.50％),but a lower incidence of eczema,lichen pilaris,freckle,chloasma and psoriasis compared with female adolescents.The incidence of acne,vitiligo,psoriasis and melasma were higher,while that of freckele was lower(25.93％ vs.29.58％ ) in Yi adolescents than in Han adolescents.Increased incidence of acne and papular urticaria and decreased prevalence of urticaria,freckle,melasma and lichen pilaris were observed in urban adolescents compared with rural adolescents.ConclusionsThe prevalence of skin diseases is high in adolescents from Liangshan prefecture.Of these 15 skin diseases,acne,freckle,lichen pilaris,eczema and urticaria are the 5 most common diseases in the area.Differences exist in the incidence of skin diseases between male and female,Han and Yi,urban and rural,adolescents.

Intrahepatic cholangiocarcinoma is the second most common intrahepatic primary liver tumor after hepatocellular carcinoma (HCC).Epidemiological study suggests a strong correlation between HBV infection and ICC development.This review focused on the potential mechanisms of HBV-induced ICC and gives a primary summary of suggested hypothesis,which included:(1) HBV infection of liver stem/progenitor cells will indirectly lead to HBV infection of intrahepatic biliary epithelial cells and lead to the development of ICC; (2) the changed microenvironment of intrahepatic biliary epithelial cells by HBV infection eventually results in carcinogenesis ; (3) the HBV infection of hepatic sten/progenitor cell can transform into tumor-like stem cells and ultimately differentiate into ICC-like tumor cells.

Objective To evaluate expression of HDAC2 in peripheral blood mononuclear cells(PBMCs)from glucocorticoid-resistant versus glucocorticoid-sensitive patients with sudden sensorineural hearing loss and identi-ty the relationship between the level of HDAC2 and glucocorticoid insensitivity.Methods PBMCs were collected from10 patients with deviation of nasal septum (control group)and 20 sudden sensorineural hearing loss patients be-fore and after intratympanic methylprednisolone for 10 days.We divided the SSNHL patients into 2 groups (GC sensitive group and GC insensitive group)according to their hearing recovery.Real time PCR and HDAC2 Assay Kit were used to detect the expression level of HDAC2 mRNA and HDAC2 activity in PBMCs.The data were analyzed with SPSS 17.0 software.ResuIts Before intratympanic methylprednisolone,the level of HDAC2 activity were sig-nificantly depressed in SSNHL patients,while the HDAC2 mRNA expressing much higher than the control group. The expression level of HDAC2 mRNA increased significantly after intratympanic methylprednisolone.The HDAC2 activity in GC sensitive group increased significantly.ConcIusion Knockdown of HDAC2 expression induces corti-costeroid in-sensitivity.Glucocorticoids can increase the expression of HDAC2 mRNA.HDAC2 activity can be down-regulated by post-translational modifications.

AIM: To deplore the adjustion of whole peptidoglycan of bifidobacterium bifidum to functions of macrophages.METHODS:Levels of interleukin 6, interleukin 12 produced by peritoneal macrophages of nude mice was detected by ELISA method, and the content of nitric oxide (NO) was detected by using Griess reagent, after whole peptidoglycan were injected into nude mice peritoneally. RESULTS:The content of interleukin 6, interleukin 12 and NO secreted from peritoneal macrophages of nude mice in the whole peptidoglycan injection group was 732.54?190.30(pg/mL)、816.37?96.40(pg/mL) and 48.90?6.51(?mol/L) respectively.It was 303.78?171.75(pg/mL), 510.27?123.46(pg/mL) and 30.67?12.83(?mol/L) respectively in the control group. In view of the content of interleukin 6, interleukin 12 and NO, there existed significant difference when whole peptidoglycan injection group was compared with control group( P

AIM: To explore the regulatory effect of bifidobacteria adolescence on NF-?B in murine peritoneal macrophages(PMs).METHODS: Macrophages were collected and were divided into tow groups,normal control group and bifidobacterium stimulation groups.The cells were fixed at 60 min after stimulated with bifidobacterium at 10~6,10~7, 10~8 and 10~9 cells/L,or fixed respectively at 15,30,60,120 and 180 min after stimulated with bifidobacterium at 10~8 cells/L,then the total protein and nucleoprotein were extracted.The activities of NF-?B and I?B? were measured with the methods of MSA and Western blotting.RESULTS: NF-?B activity markedly increased and reached the peak at 0.5 h after stimulation,while I?B? markedly decreased at same time.CONCLUSION: NF-?B activity is markedly activated by bifidobacterium.NF-?B pathway participates in the regulation of peritoneal macrophage in this process.