Objective To observe the efficacy of the sublingual immunotherapy in children with perennial allergic rhinitis caused by mites .Methods A total of 61 pediatric patients with perennial allergic rhinitis caused by mite in otorhinolaryngology clinic were collected .The children were randomized into two groups :27 cases were divided into sublingual dust mite drops group(group A) , and 34 cases were divided into conventional treatment group (group B) .The symptom scores and sign scores of allergic rhinitis were observed after 6 months ,1 year and 2 years of treatment .Both the efficacy at the end of treatment and the rate of recurrence following 2 years were compared between the two groups .Results After six month of treatment ,the symptom scores in group A was reduced to (3 .70 ± 1 .54)scores ,higher than group B(2 .82 ± 1 .40)scores ,while sign scores in group A was reduced to (1 .19 ± 0 .68)scores ,having no statistically significance compared with group B ,Both symptom scores and sign scores were not changed sig-nificantly after 1 and 2 years of treatment .The efficacy evaluation was 74 .07% in group A ,and 82 .35% in group B .The difference had no statistical difference .The recurrence rate in group A was 29 .63% following 2 years after terminal treatment ,and was superi-or to those in group B (94 .12% ) ,with significant difference(P<0 .01) .Conclusion Dust mite drops sublingual immunotherapy is effective measures for the treatment of children with allergic rhinitis caused by mites .

Objective To study the efficiency of endoscope-guided Savary bougie dilatation ( ESBD ) for strictured duodenal ulcer. Methods Forty-five patients with strictured duodenal ulcer were treated with endoscope-guided Savary bougie dilatation. The items of investigation were; easiness of passing the endo-scope through the strictured portion of duodenum, resolving of their symptoms and the complications happened after dilatation. Results The symptoms remitted and endoscope was advanced to descending portion of the duodenum through the stricture in all of 45 patients. The follow up period ranged from 6 months to 36 months. The rates of symptomatic remission were 97. 8% (44/45 ) , 97. 8% (44/45 ) , 94. 4% (34/36) , 93.7%(30/32) , 91.7% (22/24) and 87.5% (14/16) , at 6,12,18,24,30 and 36 months respectively. Recurrence of symptoms in patients without therapy was significantly higher than those with therapy. Conclusion ESBD is a safe and efficient procedure in treating the strictured duodenal ulcer.

Objective To investigate endoscopic clipping in treating acute gastroduodenal perforation. Methods Endoscopic clipping was carried out in 22 patients with acute gastroduodenal perforation as the complication of peptic ulcer or endoscopy. Results The clipping was successful in 19(86. 4% ) of 22 patients with acute gastro duodenal perforation, and surgical therapy was performed in 3(13. 6% ) of 22 cases. A single clipping was performed in 14(73. 7% ) of 19 patients resulted in satisfactory closure of perforation. An additional clipping was needed in 5(26. 3% ) patients 24 h after the first endoscopes procedure. The effectiveness of endoscopic clipping was related to location, cause and size of the perforation. Conclusion Endoscopic clipping is an effective method for acute gastroduodenal perforation as the complication of peptic ulcer or endoscopy.

OBJECTIVE To investigate the relationship among hepatitis B virus(HBV) basic core promoter(BCP) mutation,serum HBV DNA contents and HBV markers as well as liver function of chronic hepatitis B patients.METHODS The A to T mutation at nucleotide 1762 and G to A mutation at nucleotide 1764 were determined by the method of polymerase chain reaction(PCR) microplate hybridization ELISA in 176 patients with chronic hepatitis B virus infection.RESULTS The rate of HBV BCP mutants in negative and positive groups of HBeAg was 49.4% and 33.3%,respectively(P

Objective To explore the diagnostic value of the combined detection of serum Dickkopf-1 (DKK1 )and EB viral capsid antigen immunoglobulin A (VCA-IgA)in patients with nasopharyngeal carcinoma (NPC).Methods Serum levels of DKK1 and VCA-IgA were measured by enzyme-linked immunosorbent assay (ELISA)for the 80 patients with NPC and 65 normal controls.Receiver operating characteristic (ROC) curve was used to calculate the diagnostic value.Results The serum levels [M(QR )]of DKK1 in patients with NPC were significantly higher than those in normal controls [580.773 (429.1 46 )pg/ml vs.31 6.1 74 (252.965)pg/ml],with a significant difference (Z=4.846,P<0.000 1 ).ROC curves showed that the opti-mum diagnostic cutoff for serum DKK1 was 611.981 pg/ml,with an area under curve (AUC)of 0.734 (95%CI:0.654-0.81 5,50.0% sensitivity,96.9% specificity).Measurement of VCA-IgA demonstrated an AUC of 0.71 4 (95%CI:0.631-0.798,47.5% sensitivity,95.4% specificity).The combined detection of DKK1 and VCA-IgA demonstrated an AUC of 0.849 (95%CI:0.783-0.91 4,76.3%sensitivity,95.4%spe-cificity).For patients with early-stage NPC,the detection effect of combined detection of DKK1 and VCA-IgA was much better than that in normal controls,with a significant difference (χ2 =23.784,P <0.001 ). Conclusion Serum DKK1 has potential diagnostic value for NPC.Combined detection of DKK1 and VCA-IgA may aid the early diagnosis of NPC.

0\^05). Conclusion MMP\|2 and MMP\|9 may serve as indicators of invasion and malignant phenotype.Neither of them can represent the phenotype of metastasis,but both can represent that of matrix degradation.Moreover,MMP\|9 may play more important role in glioma invasion than does MMP\|2.

Objective:To observe the therapeutic effect ofexternal application ofChinese herbal medicine film plus acupoint-injection on breast hyperplasia.Methods:The 120 cases ofbreast hyperplasia with syndrome ofthe liver failing to maintain the normal flow ofqi were randomly divided into treatment group(40cases) which was treated by external application ofChinese herbal medicine film plus acupoint-injection, acupoint-injection group(40 cases) and control group(40cases).The changes ofsymptoms and signs, level ofserum sexual hormone and color doppler ultrasound analysis ofthree group were observed for comparison.Results:After 1 course oftreatment, the total effective rate oftreatment group was obviously higher than that ofcontrol group(P0.05).After 2 courses oftreatment, the total effective rate oftreatment group and acupoint-injection group was obviously higher than that ofcontrol group(P

Object To investigate the chemical constituents from the whole plant of Notoseris gracilipes Shih Methods Compounds were isolated by various column chromatography and identified by spectral analysis Results Eight sesquiterpene lactones and four other compounds were isolated and identified as jacquilenin (Ⅰ), scorzoside (Ⅱ), ixerisoside D (Ⅲ), austricin (Ⅳ), notoserolide A (Ⅴ), crepidiaside A (Ⅵ), crepidiaside B (Ⅶ), and lactuside B (Ⅷ) The other four were 6, 7 dihydroxycoumarin (Ⅸ), cichoriin (Ⅹ), luteolin 7 glucoside (Ⅺ), and daucosterol ( ⅩⅡ ) Among them, compounds Ⅰ and Ⅱ showed antibacterial activity against Bacillu cereus AS 1 1688 Conclusion All compounds were isolated from this plant for the first time

Objective To investigate the acoustic characteristics of snoring sound in patients with obstructive sleep apnea hypopnea syndrome (OSAHS) and with simple snoring. Methods 22 patients with OSAHS and 15 with simple snoring were included in this study. Natural overnight snoring was digitally recorded and portable sleep mo-nitoring was performed simultaneously. 10 snores, which were the 1st snores after 10 cycles of obstructive apnea, from each patient in OSAHS group, and 10 snores from each patient in simple snoring group were analyzed in the time and frequency domains. Results The sound waves of snoring in the two groups exhibited different patterns both in the time and frequency domains. The snoring spectrum of patients with simple snoring showed distinct fun-damental- harmonic structures which were not clear in patients with OSAHS. The central frequency of the patients with OSAHS was higher, and 800 Hz power ratio was lower than those of the patients with simple snoring. In the OSAHS group, the central frequency of the patients with moderate-to-severe OSAHS was higher, and 800 Hz power ratio was lower than those of the patients with mild OSAHS. The differences of the two parameters were of statistical significance. Conclusion The snoring sounds in patients with OSAHS and with simple snoring have dif-ferent characteristics in time and frequency domains, indicating that it is feasible to research the OSAHS by way of snore monitoring and analyzing technique.

Objective To explore the effect of nucleos(t)ide analog (NA)antiviral treatment on the pathological differentiation of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC)and the prognostic factors of HCC.Methods Totally 127 patients with HBV-related HCC who were hospitalized and received partial hepatectomy in First Affiliated Hospital of Harbin Medical University from March 2007 to November 2013 were included in this study.Sixteen cases received antiviral treatment before operation and the remaining 111 cases had no history of NA treatment.The differences of histopathological grading were compared between the two groups.Twenty-nine patients received antiviral treatment for the first time after surgery,and the rest 82 patients did not.All these patients were followed up for survival and recurrence.Multivariate analysis was used to explore the prognostic factors for HCC.The categorical variables were analyzed byχ2 test or Fisher exact test.Survival rate was compared with Log-rank test. Univariate or multivariate Cox regression analysis was used to explore the related factors of survival. Results The proportions of well-,moderately- or poorly-differentiated HCC in patients with antiviral treatment before surgery were 18.75 %,68.75 % and 12.5 %,respectively.Whereas the proportions in those without treatment were 16.22%,66.67% and 17.11 %,respectively.There was no significant difference in histopathological grading of HCC between the two groups (χ2=0.224,P =0.885 ).The overall median survival time was 39 months.The 6-month,1-and 2-year survival rates were 91 .7%, 77.5 % and 59.3%,respectively.The 6-month,1- and 2-year survival rate of postoperative antiviral treatment were 96.3%,92.4% and 78.5 %,respectively,which were significantly higher than those of no antiviral treatment group (85 .9%,70.0% and 48.5 %,respectively;χ2= 6.967,P = 0.008 ). Univariate analysis showed that tumor number,size,portal vein transfer,AFP level,postoperative antiviral treatment,histopathological grading,TNM staging,BCLC staging,γ-GT and PTA were prognostic factors for postoperative HCC survival.Multivariate analysis showed that AFP level (HR=1 , 95 %CI :1 .0004—1 .002,P =0.004),postoperative antiviral treatment (HR =0.38,95 %CI :0.38—0.15 ,P =0.04)and BCLC stage (B vs A:HR=1 .55 ,95 %CI :0.76—3.18;C vs A:HR=3.63,95 %CI :1 .31 —10.09,P =0.04)were independent prognostic factors.Conclusions Preoperative antiviral treatment has no impact on the histopathological grading of HCC. BCLC stage, AFP level and postoperative antiviral treatment are independent prognostic factors for HBV-related HCC.