1.Studies on the Diterpenoid Alkaloids of Yiyulongwutou (Aconitum pseudostapfianum)
Ying CHEN ; Lisheng DING ; Mingkui WANG
Chinese Traditional and Herbal Drugs 1994;0(01):-
From the roots of Aconitum pseudostapfianum W. T. Warig,ten diterpenoid alkaloids were isolated and identified as 14-benzoyl-8-O-methylaconine (Ⅰ ),aconitine(Ⅱ),deoxyaconitine(Ⅲ),penduline (Ⅳ),yunaconitine (Ⅴ), neolinine (Ⅵ), 15?-hydroxyneoline (Ⅶ), neoline (Ⅷ), talatisamine (Ⅸ)and aconosine (Ⅹ). I has not been previously found in nature.
3.Two new alkaloids from roots of Zanthoxylum nitidum
Xiaoling WANG ; Yanyan MA ; Keyi DING ; Lisheng DING
Chinese Traditional and Herbal Drugs 1994;0(03):-
Objective To study the chemical constituents from the roots of Zanthoxylum nitidum.Methods The constituents were isolated and purified by column chromatography.Their structures were identified on the basis of physiochemical properties and spectral data.Results Two benzophenanthridine alkaloids,1,3-bis(8-dihydronitidinyl)-acetone(1)and 8-acetonyldihydrofagaridine(2),have been iso-lated from the roots of Z.nitidum.Conclusion Compounds 1 and 2 are new compounds named nitidumtone A and nitidumtone B.
4.Studies on chemical constituents from root of Rubus innominatus
Mingkui WANG ; Jian LIANG ; Shulin PENG ; Lisheng DING
Chinese Traditional and Herbal Drugs 1994;0(04):-
Object To study the chemical constituents from the root of Rubus innominatus S. Moore (Rosaceae). Methods Twelve compounds were isolated from the methanol extract by repeated column chromatography over normal and reversed phase silica gels. Their structures were identified by analysis of their spectral data and comparing TLC with authentic samples. Results Ten triterpenic acids and two steroids were isolated and identified. The antibacterial test indicated some triterpenic acids had antibacterial activity. Conclusion All compounds were obtained from the plants of Rubus L. for the first time.
5.Chemical constituents from root of Rubus irenaeus
Rong LIU ; Lisheng DING ; Nengyu CHEN ; Mingku WANG
Chinese Traditional and Herbal Drugs 1994;0(05):-
Object To investigate the chemical constituents from the methanol extract of the root of Rubus irenaeus Focke. Methods EtOAc extraction, normal and reverse phase silica gel column chromatography were used for isolation. Spectroscopic methods ( 13 CNMR , 1HNMR , DEPT, 2DNMR, and FAB-MS) and comparison with authentic samples were used for identification. Results Ten compounds were isoated and characterized as 2?, 19?-dihydroxyl-3-oxo-urs-12-en-28-oic acid (Ⅰ), 2-oxo-pomolic acid (Ⅱ), fupenzic acid (Ⅲ), euscaphic acid (Ⅳ), 2?, 3?, 19?-trihydroxyl-olean-12-en-28-oic acid (Ⅴ), 2, 3-O-isopropylidenyl euscaphic acid (Ⅵ), pomolic acid (Ⅶ), 2?, 3?-dihydroxyl-lup-20(29)-en-28-oic acid(Ⅷ), catechin (Ⅸ) and daucosterol (Ⅹ). Conclusion These compounds were obtained from this plant for the first time.
6.CHEMICAL CONSTITUENTS FROM THE ROOT OF RUBUS CHROOSEPALUS
Rong LIU ; Lisheng DING ; Nengyu CHEN ; Mingkui WANG
Acta Pharmaceutica Sinica 2001;36(1):38-41
AIM To investigate the chemical constituents of Rubus chroosepalus Focke. METHODS The methanol extracts were suspended in H2O and then extracted with EtOAc. Column chromatography was used for separation and purification, while spectral analysis was used for identification. RESULTS Seven compounds were isolated and identified as 2α,3β-dihydroxy-urs-12,19-dien-23,28-oic acid (I), 2α,3β,23-trihydroxy-urs-12,18-dien-28-oic acid (IIa), 2α,3β,23-trihydroxy-urs-12,19-dien-28-oic acid (IIb), 2α,3α-dihydroxy-urs-12,18-dien-28-oic acid (IIIa), 2α,3α-dihydroxy-urs-12,19-dien-28-oic acid (IIIb), and the acetonide of IIIa and IIIb (IVa and IVb). CONCLUSION I was found to be a new pentacyclic triterpenoid acid.
8.Chemical constituents of Notoseris gracilipes
Xiaoxia YE ; Mingkui WANG ; Kexin HUANG ; Jiafa GUAN ; Lisheng DING ;
Chinese Traditional and Herbal Drugs 1994;0(11):-
Object To investigate the chemical constituents from the whole plant of Notoseris gracilipes Shih Methods Compounds were isolated by various column chromatography and identified by spectral analysis Results Eight sesquiterpene lactones and four other compounds were isolated and identified as jacquilenin (Ⅰ), scorzoside (Ⅱ), ixerisoside D (Ⅲ), austricin (Ⅳ), notoserolide A (Ⅴ), crepidiaside A (Ⅵ), crepidiaside B (Ⅶ), and lactuside B (Ⅷ) The other four were 6, 7 dihydroxycoumarin (Ⅸ), cichoriin (Ⅹ), luteolin 7 glucoside (Ⅺ), and daucosterol ( ⅩⅡ ) Among them, compounds Ⅰ and Ⅱ showed antibacterial activity against Bacillu cereus AS 1 1688 Conclusion All compounds were isolated from this plant for the first time
9.Studies on chemical constituents of Swertia przewalskii
Li PAN ; Xiaofeng ZHANG ; Mingkui WANG ; Zhixin LIAO ; Lisheng DING ;
Chinese Traditional and Herbal Drugs 1994;0(07):-
Object To study the chemical constituents from the whole plant of Swertia przewalskii Pissjauk Methods The constituents were isolated on silica gel column chromatography and their structures were identified by spectral and chemical analysis Results Fifteen compounds were isolated and identified as 1, 7 dihydroxyl 3, 8 dimethoxyxanthone (Ⅰ), 1, 8 dihydroxyl 3, 7 dimethoxyxanthone (Ⅱ), 1, 7, 8 trihydroxyl 3 methoxyxanthone (Ⅲ), 1, 3, 7, 8 tetrahydroxyxanthone (Ⅳ), 1 O ? D glucopyranosyl 7 hydroxyl 3, 8 dimethoxyxanthone (Ⅴ), 1 O ? D glucopyranosyl 8 hydroxyl 3, 7 dime thoxyxanthone (Ⅵ), 7 O ? D glucopyranosyl 1, 8 dihydroxyl 3 methoxyxanthone (Ⅶ), 1 O D xylo pyranosyl (1→6) ? D glucopranosyl] 7 hydroxyl 3, 8 dimethoxyxanthone (Ⅷ), 1 7 hydroxyl 3, 8 dimethoxyxanthone (Ⅷ), 1 O D xylopyranos yl (1→6) ? D glucopytanosyl] 8 hydroxyl 3, 7 dimethoxyxanthone (Ⅸ), 8 8 hydroxyl 3, 7 dimethoxyxanthone (Ⅸ), 8 O D xylopyanosyl (1→6) ? D glucopyranosyl] 1, 7 dihydroxyl 3 methoxyxanthone (Ⅹ), luteolin (Ⅺ),? sitosterol ( Ⅹ 1, 7 dihydroxyl 3 methoxyxanthone (Ⅹ), luteolin (Ⅺ),? sitosterol ( ⅩⅡ ), oleanolic acid ( ⅩⅢ ), ursolic acid ( ⅩⅣ ) and getiopicroside ( ⅩⅤ ), respectively Conclusion Compounds Ⅳ~Ⅷ, Ⅺ, ⅩⅡ , ⅩⅣ and ⅩⅤ were obtained from this plant for the first time
10.Chemical constituents from root of Glycyrrhiza uralensis
Xumin ZHU ; Yingtong DI ; Shulin PENG ; Mingkui WANG ; Lisheng DING
Chinese Traditional and Herbal Drugs 2003;34(3):198-201
Object To investigate the chemical constituents from the roots of Glycyrrhiza uralensis Fisch. Methods The constituents were isolated on normal and reversed silica gel column chromatography and their structures were identified by spectral evidence. Results A new oleanane-type triterpenoid saponin and twelve known compounds, including two triterpenoid saponins, two cumarins and eight flavonoids, were isolated. Conclusion The new compound was elucidated as 3-O-[β-D-(6-methyl) glucuronopyranosyl (1→2)-D-glucuronopyranosyl]-24-hydroxy-glabrolide on the basis of ESI-MS, 1HNMR,13CNMR, HMQC and HMBC spectral evidence.