Objective :Humna WAF1 gene was cloned,and WAF1-pcDNA3 recombinant plasmid was con-structed. Methods :Human WAF1 gene was amplified by RT-PCR from Hela cell,then cloned into pcDNA3vector. Results :Human WAF1-pcDNA3 recombinant plasmid was constructed,and DNA direct sequencingindicated that was correct. Conclusion:Our study laid the foundation for studying WAF1 gene expressionand it＇s biological effects.

OBJECTIVETo establish cardiac magnetic resonance imaging(MRI) derived left ventricular (LV) global and region function parameters in normal adults.

METHODSTwenty normal adults were examined with fast imaging employing steady-state(Fiesta) acquisition sequence of cardiac MRI, LV global function and LV region function were measured at basal, middle, apical level and at 16 LV segments. The regional function parameters among different levels and different segments of the same level were analyzed.

RESULTS(1)LV global function: end-diastolic volume (109.17 ± 19.52) ml; end-systolic volume (37.76 ± 14.16) ml;ejection fraction (65.93 ± 7.79) %; wall thickening (83.24 ± 40.82) %; longitudinal shortening (15.51 ± 3.78) %; fractional shortening (31.78 ± 9.55) %;end-diastolic mass (95.20 ± 19.95) g. (2)LV regional function: In each LV level, there was no significant difference in end-systolic wall thickness (P > 0.05). End-diastolic wall thickness and wall thickening were similar between the middle and apical levels, but there were significant differences between middle and apical levels with the basal level(both P < 0.05). End-systolic wall thickness of the middle and the apical level was similar, but there were significant differences between middle and apical levels with the basal level (both P < 0.05). At the segments of the same level, end-diastolic wall thickness and the relevant regional function parameters between the segments of anteroseptal and inferoseptal at base and middle level were similar (P > 0.05); the end-diastolic wall thickness was the largest and the WT was the minimal at the septal segments of three levels, and the difference were significant between the septal and other segments in the same level (P < 0.05).

CONCLUSIONSFractional shortening and longitudinal shortening provide new indicators for assessing LV global function by cardiac MRI. There is obvious heterogeneity on LV regional function in normal adults, systolic function is the strongest in apical level and the weakest in spetal segments of LV.

Adult ; Female ; Healthy Volunteers ; Heart Ventricles ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Ventricular Function, Left ; Young Adult

OBJECTIVETo evaluate the left ventricular (LV) radial and longitudinal systolic function in hypertrophic cardiomyopathy (HCM) patients by 3.0 T MR.

METHODSSixteen HCM (HCM group) and twenty normal adults (normal group) were examined with fast imaging employing steady-state (FIESTA) acquisition sequence of cardiac MRI. LV ejection fraction (LVEF), longitudinal shortening (LS) and fractional shortening (FS) in three standard levels were measured to analyze LV radial and longitudinal systolic function.

RESULTSAsymmetric hypertrophy was detected in all HCM patients. The LVEF and FS were significantly higher while LS was significantly lower in HCM group than those in normal group (P < 0.05 or 0.01). FS at basal and middle levels were significantly higher in HCM group than in normal group (both P < 0.01). FS in apex level was similar in the two groups (P = 0.057). Pearson correlation analysis showed that LS was negatively related with the number of hypertrophy segments in HCM patients (r = -0.537, P = 0.032). But there was no correlation between FS and the number of hypertrophy segments as well as FS and LS in HCM patients (r = -0.090, P = 0.739; r = 0.049, P = 0.856).

CONCLUSIONThe LV longitudinal systolic function was reduced but the LV radial systolic function remained unchanged in HCM patients, thus, LS changes could better reflect myocardial systolic function in HCM patients.

Adult ; Cardiomyopathy, Hypertrophic ; physiopathology ; Heart Ventricles ; Humans ; Magnetic Resonance Imaging ; Myocardium ; Systole ; Ventricular Dysfunction, Left ; Ventricular Function, Left

Purpose: Patients with advanced biliary tract cancer (BTC) have a poor survival. We aim to evaluate the efficacy and safety of nab-paclitaxel plus gemcitabine and cisplatin regimen in Chinese advanced BTC patients. Materials and Methods: Eligible patients with locally advanced or metastatic BTC administrated intravenous 100 mg/m2 nab-paclitaxel, 800 mg/m2 gemcitabine, and 25 mg/m2 cisplatin every 3 weeks. The primary endpoint was progression-free survival (PFS). The secondary endpoints included overall survival (OS) and adverse events, while exploratory endpoint was the association of biomarkers with efficacy. Results: After the median follow-up of 25.0 months, the median PFS and OS of 34 enrolled patients were 7.1 months (95% confidence interval [CI], 5.4 to 13.7) and 16.4 months (95% CI, 10.9 to 23.6), respectively. The most common treatment-related adverse events at ≥ 3 grade were neutropenia (26.5%) and leukopenia (26.5%). Survival analyses demonstrated that carcinoembryonic antigen (CEA) levels could monitor patients’ survival outcomes. A significant increase in the number of infiltrating CD4+ cells (p=0.008) and a decrease in programmed death-1–positive (PD-1+) cells (p=0.032) were observed in the response patients. Conclusion In advanced BTC patients, nab-paclitaxel plus gemcitabine and cisplatin regimen showed therapeutic potential. Potential prognostic factors of CEA levels, number of CD4+ cells and PD-1+ cells may help us maximize the efficacy benefit.