0.05).Conclusions:The incidence of CIN and especially high-grade CIN are higher in patients with positive high risk types of HPV-DNA than negative. HPV-DNA testing has significance in the diagnosis of cervical displasia;HPVDNA testing combined with pap smear and colposcopy can increase the diagnosis of cervical diaplasia.

In type 2 diabetic patients the f re quencies of point mutations 3316G→A and 3394T→C in mitochondrial DNA (mt DNA) were 3.9% (6/152) and 5.3% (8/152) respectively, which were significantly higher than those in normal controls and patients with coronary heart disease. This fi nding suggests that mtDNA 3316G→A and 3394T→G mutations are related to type 2 diabetes.

After the types and characteristics of medical and health information websites in China were described , the problems existed on these websites and the reasons why they occurred were analyzed with some suggestions pro-posed for the development of medical and health information websites in China , such as controlling them by national policies and regulations, working out quality control standards, encouraging and supporting high level websites, es-tablishing needs-based medical and health resources database .

Objective To investigate the relationship between genetic polymorphism of N-acetyltransferase 2(NAT2) and susceptibility to bladder cancer.Methods Based on case-control study,NAT2 mutation alleles(NAT2*5,*6 and*7) were determined by ASPCR and PCR-RFLP in 78 patients with bladder cancer and 80 nontumorous patients.In addtion,the relationships between the genotypes and tobacco smoking,occupational exposure,high dose intake of meat or pathological characteristic of bladder cancer patients were analyzed.Results In the blood samples from 158 cases,the 4 alleles NAT2*4,NAT2*5,NAT2*6 and NAT2*7 were detected.The frequency of NAT2 slow genotypes was 29.5%(23/78) in patients with bladder cancer,which was significantly higher compared with 16.3%(13/80) in control patients(P

Objective To approach the effect of acrylonitrile (ACN) on the proliferation and differentiation of the lung fibroblasts of mice. Methods The purification and primary culture of the lung fibroblasts(LFb)of new born mice were conducted. The cells were treated with ACN added in the medium at the varying doses of 0.01, 0.5, 1.0, 10.0, 50.0, 100.0 and 200.0 ?g/ml. The cytomorphological methods were used, the protein, malonaldehyde (MDA) and the activity of superoxide dismutase (SOD) were determined. Results No inhibitted effect on the lung fibroblasts was observed at the doses of 0.01-10.0 ?g/ml. At the doses of 50.0-200.0 ?g/ml, acrylonitrile could inhibit the differentiation and proliferation of the cells, the volume of cells became small,the rate of cell clusters decreased, at the doses of 10.0-200.0 ?g/ml, the content of protein and the activity of SOD decreased, MDA content increased. Conclusion Acrylonitrile can inhibit the proliferation and differentiation of the lung fibroblasts in mice, protein synthesis inhibition and lipid peroxidation are considered as the related factors.

2.5-7.0,a close correlation can be seen between the indoor and outdoor air.

Synovial sarcoma is a malignant soft tissue with unknown origin. Although head and neck region is the second common site of involvement, rare cases have been reported in Para nasal sinus and larynx. We presented two cases of synovial sarcoma, one of which arised from maxillary sinus and the other from laryx, and re- view the literature to sum up the diagnosis and treatment strategies. The conclusion is that synovial sarcoma in the head and neck still raises diagnostic and therapeutic issues. Surgical excision with wide margins is essential and necessary, usually associated radiotherapy. The effect of chemotherapy remains to explored.
Head and Neck Neoplasms ; pathology ; Humans ; Larynx ; Maxillary Sinus ; Paranasal Sinuses ; Sarcoma, Synovial ; pathology

Objective To investigate the change of surface molecules and cytokine expressions of dendritic cells (DC) stimulated with recombinant proteins Com1 and heat shock protein B (HspB) of Coxiella burnetii (Cb).MethodsThe DC cultured for 5 d were stimulated with 20 μg/mL recombinant protein Com1,20μg/mL HspB or 6 μg/mL E.coli lipopolysaccharide (LPS).Twentyfour hours later,the surface mature marker,CD83,and activation-associated markers of T lymphocytes,CD58,CD54,CD40,CD80 and CD86,and expression levels of interleukin (IL)-10 and IL-12 of DC were detected by fluorescence activated cell sorter (FACS).Multiple comparisons were performed by using Student-Newman-Keuls test.ResultsCom1 could induce DC maturation efficiently in vitro.Human monocyte-derived DC exhibited significantly higher expression levels of surface molecules including CD83,CD54,CD58,CD80,CD86,and CD40,which were all ＞80％.CD83 expression induced by Com1 was similar with that induced by 1E.coli LPS,while CD54 and CD86 expressions were slightly higher than those induced by E.coli LPS,and expressions of other molecules were significantly higher than those induced by E.coli LPS (all P＜0.05).After Com1 stimulation,intracellular IL-12 level increased to 9 ％ from zero.HspB could not induce DC maturation in vitro.The intracellular IL-10 level was 6％ after HspB stimulation.DC pulsed with Com1 and HspB exhibited intracellular IL-12 level of zero and IL-10 level of ＜2％.Conclusion Com1 but not HspB can efficiently activate DC and Com1-activated DC can drive T cells toward Th1 cell development due to a high level of IL-12 production.

Objective To retrospectively study the results of endoscopic manifestation pathology,and treatment of colonic polyps. Methods Clinical data of 121 patients diagnosed with colonic polyps and treated by endoscopy between November 2002 and September 2009 were studied. Complete colonic investigation was done by Olympus PCF240I electron endoscopy. Colonic polyp excision was done by high frequency electric coagulation. Results All patients had bloody stool with mucus for 1 week to 8 years. Ninty four(77.7%)patients had a single polyp,6(5.0%)had two polyps,21(17.3%)with multiple polyps,and 5 patients had familial polyposis coli. The size of the polyps ranged from 0.3 cm to 4 cm and the morphology of Yamada Ⅰ to Ⅳ. The pathological changes were juvenile polyps,inflammatory polyps,polypoid formation,and canalicular adenoma. Most common pathology types were Yamada Ⅳ and juvenile polyps,107 of the 121 patients(88.4%)had juvenile polyps,and one was found to have malignant transformation. 115 patients accepted high frequency electric coagulation excision under general anesthesia,without complication. Conclusions Colonic polyp is one of the common diseases in children. Children should be checked by total colonoscopy in time when they present with bloody stool,anemia,rectal polyps prolapse or intussusception. Polypectomy under endoscope is a most safe and reliable treatment method when the diagnosis is confirmed. For isolated juvenile polyp,regular recheck is not necessary after polypectomy except when bloody stool recurs. Regular follow up by colonoscope is necessary for juvenile polyp combined with adenoma and adenomatous polyps. Colonoscope is necessary at any time when bloody stool occurs. Familial polyposis coli is difficult to be treated by endoscopy,thus surgical procedure is suggested.

Objective To compare the prognosis effect of different Helicobacter pylori (Hp)eradication methods in patients with chronic gastritis.Methods One hundred and twenty patients with chronic gastritis diagnosed by gastroscopy and pathology examination were divided into sequential therapy group (36 cases),triple-combined therapy group (34 cases),Hp positive without eradication group (30cases) and Hp negative group (20 cases) according to the Hp infection status.All patients were followed up by gastroscopy,biopsy and rapid urease test before and after therapy.Results The Hp eradication rate in sequential therapy group was 94.44％ (34/36),in triple-combined therapy group was 73.53％ (25/34),and there was statistical difference (x2 =5.775,P =0.016).The symptoms scores and gastroscopy scores after therapy in the four groups were significantly lower than those before therapy,and there were statistical differences (P ＜ 0.05).The symptoms scores and gastroscopy scores after therapy in sequential therapy group were significantly lower than those in triple-combined therapy group,and there were statistical differences (P ＜0.05).The symptoms scores and gastroscopy scores after therapy in sequential therapy group,triple-combined therapy group and Hp negative group were significantly lower than those in Hp positive without eradication group [(0.84 ± 0.60),(1.34 ± 0.59),(1.49 ± 0.62) scores vs.(2.98 ± 0.54) scores,(0.47 ± 0.37),(0.83 ± 0.35),(0.96 ± 0.75) scores vs.(1.22 ± 0.40) scores],and there were statistical differences (P ＜ 0.05).Conclusions The Hp eradication rate of sequential therapy is higher than that of triple-combined therapy.The two therapy methods can both improve the symptoms score and gastroscopy score,but the symptoms after sequential therapy are relieved faster compared with triple-combined therapy.