1.EFFECT OF VITAMIN A ON THE LUNG METASTASIS IN C_(57)BL MOUSE MELANOMA
Chinese Pharmacological Bulletin 1986;0(04):-
Effects of vitamin A on antimetastatic activity of alveolar M 0 were studied. The results were as follows:1 . SD rats recieved vitamin A were killed and their alveolar M0 were harvested by lavage and cocultured with prelabelled B16 Melanoma cells for 48h in proportion of 20 : 1 , the average precentage of cytotoxity were 34.28%;2 . Zymosan-C3b rosette formation in SD rats treated with vitamin A was significantly increased. The average rosette forming rate was 43.00%. It was markdly higher than those of controls ( P
2.Determination of eugenol in Bawei Tanxiang Pills by Capillary Gas Chromatography
Wei TIAN ; Zhaohui CHENG ; Chunhui LIU ; Yongming LI ; Liren CHENG
Chinese Traditional Patent Medicine 1992;0(08):-
AIM: To establish a capillary GC-FID programmed temperature method for determining eugenol in Bawei Tanxiang Pills(Lignum Santali Albi, Flos Caryophylii, etc.). METHODS: Chromatography was performed on a 25m?0.25mm(0.25?m film thickness), SE-54 fused-silica capillary column coupled with a FID-detector. The internal standard was cyclohexanone. RESULTS: A good linearity was obtained in the range of 0.25?g~8?g of Eugenol with a correlation coefficient of 0.9993. The average recovery was 96.5%, the RSD was 2.4%. CONCLUSION: The established method was provided to be a reliable quantitative method for quality control of Bawei Tanxiang Pills.
3.Determination of Content of Ginsenoside R_(g1) ,R_e, and Paeoniflorin from Shenshao Tablet by HPLC
Shilan FENG ; Yuming DONG ; Shengyu DUAN ; Shouwei QU ; Yongmin LI ; Liren CHENG ;
Chinese Traditional Patent Medicine 1992;0(01):-
Objective:To determine the content of ginsenoside R g1 ,R e and Paeoniflorin by HPLC in Shenshao tablet.Methods: HPLC Symmetry C 18 column was used and CH 3CN H 3PO 4 (11∶89) as mobile phase. The flow rate was 0.8mL/min, the column temperature at 30 ?C and the detective wavelength 203nm and 230nm. Results:The content of ginsenoside R g1 ,R e, and paeoniflorin can be determined by HPLC. The linear concentration ranges of these compound were 0.960?g~4.800?g, 0.912?g~4.560?g,1.050?g~ 5.250 ?g. The average recoveries were 105.2%, 98.5% and 103.5%, respectively.Conclusion: This method is simple and accurate and can be used to determine the content of Shenshao Tablet.