Objective To evaluated the sexual psychology changes and marriage quality for ovarian cancer patients after operation and supply selective psychological nursing intervention for patients and their family members.Methods Self-designed questionnaire was used to investigated ovarian cancer patients under 60 years old one month after operation to understand the sexual psychology changes and its related factors.Nursing intervention was adopted according to different conditions of patients.Results Factors that influenced the sexual psychology of patients included:age,education degree,chemotherapy process,clinical symptom,anxiety and depression status,marital status and degree of nursing intervention.Conclusions Sexual psychology Was influenced by many factors.We should adopt various kinds of nursing intervention to promote the physical and mental health of patients,facihtate the recovery of sexual function and improve their life quality.

A survey and analysis were conducted at the end of 2002 of urban community health service institutions in 31 provinces, autonomous regions and municipalities directly under the Central Government so as to find out about the basie situation of community health service institutions across the country. The result indicated that by the end of 2002, the number of cities that had conducted community health service work reached 358. The coverage of community health services in cities of the prefecture level, areas under the jurisdiction of a city and cities of the county level was respectively 92. 1%, 80. 3% and 28.0% . 12 132 community health service institutions were set up in 2002, marking an increase of 31.6% as compared with 2001. The growth of community health services was fast and yet uneven in various regions. It is suggested that more efforts be made to strengthen community health services in medium-sized and small cities and improve the management of institutions and the system for statistical analysis of in-formation.

Objective Cloning of some specific genes related to the prostate cancer. Methods Specimen from a patient with prostate cancer and from a normal adult were studied by the improved mRNA differential display and the differentially expressed sequence-tags were cloned,sequenced and analized.Reverse transcriptive-polymerase chain reaction(RT-PCR) was used to examine the expression level of Clusterin in prostate cancer tissues,prostate cancer cell line and normal prostate tissues. Results Significant difference was observed between the two kinds of tissues in gene expression and seven differentially expressed sequence-tags were found through analysis in GenBank.Among them,five were proved to be new gene tags,one shared 100% homology with Clusterin,which was highly expressed in the prostate cancer tissue and the other one shared 97% homology with superoxide dismutase 1 (SOD1),which was highly expressed in the normal prostate tissue.It was proved by RT-PCR that to compare with the internal marker gene ?-actin,the expression level of clusterin in prostate cancer is much higher than that of normal prostate. Conclusions Five new gene tags,clusterin and SOD1 were found to be differentially expressed between tissues of prostate cancer and normal prostate and may play important roles in carcinogenesis and development of prostate cancer.

Objective To detect differentially expressed genes in prostate cancer by cDNA microarray. Methods Total RNA was isolated by one step protocol from prostate cancer (CaP) and from normal prostate,and Poly(A) +RNA was further purified through Oligitex mRNA Midi Kit.Then it was analysed for differentially expressed genes in CaP and normal prostate by cDNA microarray with 4 096 human genes. Results There were 341 differentially expressed genes between CaP and normal prostate,of which there were 128 down regulated and 213 up regulated ones for CaP. Among these genes,15 were the most significant with 6 down regulated and 9 up regulated. Conclusions cDNA microarray can be used effectively to find out differentially expressed genes in prostate cancer which is not regulated by any single gene.Many different kinds of genes are involved in the initiation and evolution of CaP carcinogenisis.

Objective To study the expression and significance of antiapoptosis facter-clusterin in prostate neoplasm. Methods Reverse transcriptive polymerase chain reaction(RT PCR) was used to examine the expression level of clusterin in prostate cancer tissues(3 cases),prostate cancer cell line PC3M and normal prostate tissues(3 cases). Results Compared with the internal marker gene ? actin , the expression level of clusterin in prostate cancer is much higher than that of normal prostate. Conclusions The high expression level of clusterin in prostate cancer indicates that clusterin may play an important role in the biological characteristics of prostate cancer through the antiapoptosis pathway.

Objective The diagnosis and treatment of renal angiomyolipoma were studied. Methods A total of 72 cases of renal angiomyolipoma encountered and treated from Jan. 1983 to Dec. 1999 were reviewed. Results Of the 72 cases, 13 were misdiagnosed preoperatively.The positive diagnosis rate of ultrasonography was 73.6%(53/72) and that of CT 81.9%(59/72).10 cases( 13.9 %) had been misdiagnosed preoperatively with the combined use of ultrasonography and CT.Tumors smaller than 4 cm had a significantly higher misdiagnosis rate( P

Objective To establish age specific reference ranges of serum total prostatic specific antigen (T PSA) and to investigate the relationship between T PSA,free PSA (F PSA) and ratio of F PSA/T PSA(F/T) with age in benign prostatic hyperplasia (BPH) patients. Methods 913 consecutive cases were studied retrospectively.In all of them with the age ranged from 30 to 89, serum T PSA and F PSA levels were assayed.All the patients were suspected to be BPH or PCa,103 being diagnosed as PCa on biopsy under B ultrasound and the other 810 being BPH. Results The incidence rate of PCa was 11.3% in this group.Serum T PSA and F PSA levels were positively correlated with the age ( r=0.22,P

Objective To compare the serum PSA (T PSA and F/T PSA ratio) between patients of benign prostatic hyperplasia (BPH) and prostate cancer (PCa). Methods We studied retrospectively 810 cases of BPH and 103 cases of PCa consecutively from November of 1996 to June of 1999 and compared the differences of T PSA level and F/T ratio between these two kinds of diseases. Results In BPH patients,serum T PSA 10 ng/ml were 71.7%, 22.1 % and 6.2% respectively while these were 10.7%,17.5% and 71.8% in PCa patients ( P 10 ng/ml(0.110 ng/ml.

Objective To analyze the cause of re-operation after radical cystectomy and ileal conduit in early follow-up period in order to improve the efficacy of this operation and reduce postoperative complications. Methods 12 cases from 81 cases of re-operation were studied retrospectively after radical cystectomy and ileal conduit for bladder tumor in early follow-up period.Of the 12 cases,there are 4 cases of T 4,8 cases of T 3,7 cases of G 2,5 cases of G 3.They are all transitional cell carcinoma. Results There were 4 cases of adhesive intestinal obstruction,2 necrosis of ileal conduit,2 adhesive folding of sigmoid colon into prostatic fossa,2 internal hernia and 2 avulsed incision due to infection .All the cases were re-operated nearly the first operation.After the second operation,all the cases were followed 6～12 months,average 7.7 months,the outcome was satisfied. Conclusions The ileal conduit is a kind of ideal methods for urinary diversion and its early complications should be avoided affecting the efficacy of this operation and the quality of life patients.

Objective To study the killing effect of hypocrellins B-photodynamic therapy (HB-PDT) for lung cancer cell line A549, to compare with that of hematoporphyrin derivative-photodynamic therapy (HpD -PDT), in order to define the superiority of HB-PDT in the therapy of lung cancer. Methods Lung cancer cell line A549 was used in the study. The cells were incubated in vitro with HB or HpD dissolved in DMEM in different concentrations, and then irradiated by copper vapor laser with mixed wavelength light under saturated light dose. Cell survival rate was respectively measured by MTT assay after 24 hours' incubation. According to cell survival curves after being cultured with different photosensitizers in different concentrations, the equation of each cell survival curve was plotted. Finally IC 50 (50% inhibition concentration) of each photosensitizer was derived. Results The results showed that HB-PDT had a strong killing effect on lung cancer cell. The IC 50 of HB was 33.82ng/ml for lung cancer cell, while the IC 50 of HpD was 1 316.88ng/ml, which was 38.94 fold of that of HB. Conclusions HB was a more effective photosensitizer than the photosensitizer HpD. HB-PDT had strong photodynamic killing effect on lung cancer cell line.