Objective To study the dose of IVGG in treating atypical Kawasaki disease(KD) at defervescence and the necessity.Methods Clinical data of 54 cases of atypical Kawasaski disease without coronary artery lesion (CAL) but with the abatement of fever in 10 days were retrospectively analyzed. They were divided into there groups according to IVGG dosage ;group A (1g/kg IVGG n=11 ),group B(2g/kg n=24 ) and group C (blank n=19 ) .CAL was observed to at 14~21 days and 0.5year. Results Compared with group A and B, The rate of CAL was significantly higher (P0.05) . Conclusions In order to prevent CAL ,atypical Kawasaki disease(KD) children with confirmed diagnosis in 10 days should all be treated with IVGG whether with abatement of fever or not .The dose of 1g/kg IVGG would have satisfactory effects on atypical KD children at defervescence.

Objective: To study the mechanism of safflower injection(红花注射液) on preventing thrombosis after arteriovenous fistula operation in rabbits.Method: Twenty-four rabbits were randomly divided into three groups: safflower injection group,heparin group and control group(each n=8).Rabbit femoral arteryvein side-to-side anastomosis models were performed on both sides of the lower extremities and they were coincided.Safflower injection(5 mg/kg) or heparin injection(6 U/kg) was given from ear edge vein immediately after operation in the respective group,while normal saline(0.9%) was given with the same fashion in the control group,once per day for 14 days.On the 1 st,3 rd,7 th and 15 th day after operation,the nitric oxide(NO) in serum and endothelin(ET) in plasma were determined respectively.Results: The plasma ET contents were significantly lowered in safflower and heparin groups compared with that of control group(both P

5cm hepatic cancer, the effective rate (CR+PR) was 60 8% (62/102), no change (NC) rate 39 2% (40/102), and the 1-, 2-, 3-year survival rates 67 6% (69/102), 49 1% (50/102) and 39 2% (40/102), respectively. The effective rates (CR+PR) in patients with bone tumor and lung cancer were 100%(12/12) and 60%(3/5), respectively. There were no other severe complications found. Conclusions Ultrasound-guided microwave intervention is a safe, effective and feasible method for hepatic cancer, lung cancer and bone tumor.

Objective To analyze regularities in selection of analogous points for acupuncture and moxibustion treatment of restless legs syndrome in modern Chinese literature. Three large Chinese databases, China National Knowledge Infrastructure (CNKI), Wan Fang database (Wanfang) and VIP Database (VIP), were searched by computer to retrieve literature on acupuncture and moxibustion treatment of restless legs syndrome. A database of acupuncture and moxibustion prescriptions was set up. The characteristics of and regularities in selection of analogous points for acupuncture and moxibustion treatment of restless legs syndrome were analyzed by use of data mining techniques. Results A total of 107 related articles were included, involving 105 selected acupoints. The main analogous points for modern acupuncture and moxibustion treatment of restless legs syndrome are Zusanli, Sanyinjiao, Chengshan and Yanglingquan. The main meridians that the analogous points belong to are the Bladder Meridian of Foot-Taiyang, the Gallbladder Meridian of Foot-Shaoyang and the Spleen Meridian of Foot-Taiyin. Most of these acupoints are distributed on the lower limb and the lumbodorsal region. The most frequently used specific points that the analogous points belong to are Five Shu points and lower He-Sea point. Association rule analysis shows that the points that are most highly correlated are Yanglingquan and Zusanli. Attention is paid to acupoint selection based on syndrome differentiation and compatible application of local acupoints.Conclusions Selection of analogous points that have the same or similar indications for modern acupuncture and moxibustion treatment of restless legs syndrome makes acupoint combinations produce a synergistic effect and can improve the clinical therapeutic effect.

Objective To get a more comprehensive view of caregiving experiences, and describe the caregiver burden and the positive aspects of caregiving of family caregivers of senile dementia patients in community and to explore the relationship between the caregiver burden and the positive aspects of caregiving. Methods Totally 80 family community-dwelling caregivers of senile dementia patients in community were investigated by Caregiver Burden Inventory (CBI) and Positive Aspects of Caregiving (PAC) questionnaires. Results The score of CBI was 54.73 ± 13.15 and the score of PAC was 28.53 ± 5.37. The score of PAC was negatively correlated with the caregiver burden (r=-0.243, P<0.05). Conclusions The caregiver burden of the family community-dwelling caregivers of dementia patients is high and the positive aspects of caregiving is at middle level. Professional help and support should be provided to the family caregivers of dementia patients by the community health service centers on the premise that the caregiving experience is fully understood. Our aim is to reduce their burden, strengthen the caregiving skills and improve the positive aspects of caregiving simultaneously.

Objective To construct and identify the efficacy of a lentiviral vector harboring RNAi sequence targe-ting NSBP1 gene. Methods Three siRNA targeting the NSBP1 mRNA were designed, the pGCSIL-GFP-NSBP1 lentivirus vectors were constructed and confirmed by DNA sequencing. A total of 293T cells were co-transfected with pGCSIL-GFP-NSBP1, pHelper1.0 and pHelper2.0 for the virus stocks produced, the titer of the virus was test-ed. After lentivirus transfecting into DU145 ceils, Western-blot and MTT methods were used to determine the ex-pression and biological activity of NSBP1 gene, the cells were transplanted into nude mice, then inhibitive effect was observed. Results PCR analysis and DNA sequencing demonstrated that the RNAi sequence targeting the hu-man NSBP1 gene was successfully inserted into the lentiviral vector. The titer of the recombinant]entiviral vector was 2 × 10~8TU/mL. NSBP1 protein expression level in transfected cells was significantly decreased and growth rate of cells transfected with lentivirus was decreased by MTT assay, the downregulation of NSBP1 reduced growth rate of transplantated tumor, whereas tumorgenicity was not influenced. Conclusion The construction of the]entiviral vector of NSBP1 has been successfully prepared and NSBP1 plays an important regulatory role in androgen-inde-pendent prostate cancer cell proliferation.

Objective:To observe the expression of Th1 versus Th2 type cytokines in primary hepatic cancer(PHC)and its adjacent liver tissues.Methods:The gene expression of Th1/Th2 cytokines was detected by RT-PCR using IFN-?and IL-2 as Th1 type cytokine genes,IL-4 and IL-10 as Th2 type cytokine genes.Results:Thl type cytokines were expressed in 7 and 9 cases,while Th0 type cytokines in 4 and 2 among 11 PHC and their adjacent liver tissues,respectively.Conclusion:Th1 type cytokines are expressed predominantly in primary hepatic cancer and its adjacent liver tissue.

Objective To investigate the relationships between the expressions of estrogen receptor(ER),progesterone receptor(PR),carcinoembryonic antigen(CEA),c-erbB-2,and clinicopathologic parameters of the breast cancer and to evaluate their value in predicting the prognosis of breast cancer.Methods The databank of clinical data and expressions of ER,PR,CEA and c-erbB-2 proteins in 791 cases of breast cancer were established and the results were analyzed by using chi-square test,Kaplan-Meier(SPSS10.0).Results The positive expression rates of ER,PR,CEA and c-erbB-2 were 64.6%,66.5%,45.5% and 66.2% in the 791 breast cancers,respectively.The positive rate of ER expression in the infiltrating lobular carcinomas was significantly higher than that of the infitrating ductal carcinomas of breast(P

Objective To study current situation and existed problems in the public health emergency alert and response system so as to formulate more advices to consummate it and help to establish a more reasonable system adapting to our country. Methods A qualitative study was carried out among CDC working staff including two focus group discussions(FGD). Results Although government had put more emphasis on public health,increasing its devotion and strengthening the alert and response capability after SARS outbreak, some problems still existed, such as ① absence of correlative laws and rules; ② lack of government devotion; ③ insufficient utilization of surveillance information; ④ lack of the canonical response guidance. Conclusions With regard to the building up of the public health emergency alert and response system, the following proposals are put forward: increasing payout on public health; consummating the construction of disease surveillance network; affording more education and training to correlative staff engaged in the disease control to improve their abilities.

Objective To investigate the effects of enriched environment and impoverished environment on brain function after hypoxic-ischemic brain damage(HIBD).Methods Three-day-old SD rats,which were divided into enriched environment group(EE),standard environment group(SE) and impoverished environment group(IE) by random digits table were used to establish HIBD model.The sham-operation rats served as control group.Different environment stimulations were administrated to the rats respectively since the 2nd day after HIBD.On the 32nd day,hanging test and incline plane test were carried out to evaluate the sensorimotor function.Morris water maze was used to evaluate the learning and memory ability.The expression of GFAP in the cortex and hippocampus was measured by the method of immunohistochemistry.Results The brain function of IE group was much worse than that of SE group(P0.05).Immunohistochemical analysis showed the expression of GFAP in the cortex and hippocampus of Sham group was significantly lower than that of other groups(P