Objective To study the effects of broad-spectrum antibiotics, including the third generation Cephalosporin and Carbapenems, on intestinal enterococci in septic rats. Methods Ninety-one SD rats were randomly divided into normal control group, scald group, scald group with 3 days of ceftriaxone treatment, scald group with 9 days of ceftriaxone treatment, scald group with 3 days of imipenem treatment, scald group with 9 days of imipenem treatment, and sepsis group, sepsis group with 3 days of ceftriaxone treatment, sepsis group with 9 days of ceftriaxone treatment, and sepsis group with 3 days of imipenem treatment, sepsis group with 9 days of imipenem treatment. All the animal were subjected to 30% TBSA Ⅲ degree scald injury on their back, followed by endotoxin challenge with a dose of 20mg/kg 24 hours after the burn injury. The animals were treated with intraperitoneal injection of ceftriaxone (60mg/kg, q12h) or imipenem (60mg/kg, q12h) 24h after the second hit with endotoxin. At the end of the treatment, intestinal enterococci in colon were cultured. The number and variety of the bacteria were also determined. Results The number of enterococci in the gut of the septic rats was significantly increased after the second hit with endotoxin, exceeding that of the rats in normal control group and scald group(P

Objective To study the effects of broad-spectrum antibiotics, including the third generation Cephalosporin and Carbapenems, on intestinal commensal bacilli in rats with sepsis. Methods Ninety-one SD rats were randomly divided into normal control group, scald group, scald group with 3 days of ceftriaxone treatment, scald group with 9 days of ceftriaxone treatment, scald group with 3 days of imipenem treatment, scald group with 9 days of imipenem treatment, and sepsis group, sepsis group with 3 days of ceftriaxone treatment, sepsis group with 9 days of ceftriaxone treatment, and sepsis group with 3 days of imipenem treatment, sepsis group with 9 days of imipenem treatment. All the animal were subjected to 30% Ⅲdegree scald injury on their back, followed by endotoxin challenge with a dose of 20mg/kg 24 hours after the burn injury. The animals were treated with intraperitoneal injection of ceftriaxone (60mg/kg, q12h) or imipenem (60mg/kg, q12h) 24h after the second hit with endotoxin. At the end of the treatment, bacilli in the colon were cultured. The number and variety of the bacteria were also determined. Results The antibiotics obviously reduced the number of colibacilli(P

Objective To investigate the relationship between cerebral infarction (CI) and the single-nucleotide polymorphisms (SNPs) of rs11833579G/A and rs4980959C/A of NINJ2 gene in Han population of the Western Guangdong province in China. Methods Genotype and allele frequency of rs11833579G/A and rs4980959C/A were analyzed in 278 cerebral infarction patients and 120 healthy controls. Results There was insignificant difference in frequencies of genotypes and allele in two polymorphisms (rs11833579 G/A and rs4980959C/A) of NINJ2 gene between CI and control groups (P>0.05). AA genotype frequency of rs4980959C/A was more in patients with small-artery occlusion (SAA) than in the control group (18.3% vs 9.2%, P=0.041), while the A allele frequency was more in the patients with SAA than in the control group (35.4% vs 46.1%, P=0.019). Logistic regression analysis indicated that AA genotypes of rs4980959C/A was a risk factor for SAA over G allele carriers (OR=4.012, 95%CI: 1.209~14.939, P=0.027). Conclusion NINJ2 gene 5' upstream untranslated region SNPrs11833579G/ A polymorphism does not associate with the risk of ischemic stroke. NINJ2 gene 5' upstream untranslated region SNPrs4980959C/ AA allele is a risk factor for SAA, AA genotype is a susceptible genotype for SAA.

Objective To study the pathogenicity of ureaplasma urealytieum serotype 3 (UU3) with different concentration in the genital tract of the mice. Methods A total of 156 Kunming mice were divided into 4 groups randomly, including group A, B, C (48 mice in every experimental group) and control group.(12 mice in control group). UU3 at concentration of 1×107eopy/g (group A), 1×106copy/g (group B),1×105copy/g (group C) were inoculated into 48 mice in every experimental group intravaginal]y, in the mean time, culture medium of UU was given into 12 mice in control group. They were neeropsied at 1, 3,7, 14, 21, 35 days of postinoeulatien randomly, which included 8 mice of every experimental group and 2 mice of control group every time, and to detect UU3 expression from cervical secretions by FQ-PCR andobserving the pathogenicity rate in tissues of cervix, endometrium, fallopian tube by light microscope and calculate the morbidity rate. Results (1) The total positive rates of UU3 were 63% (30/48) in group A,50% (24/48) in group B, 17% (8/48) in group C, which showed a significant difference(P<0.01).And at 1,3,7,14,21,35 days of postinoculation, the positive rates of group A were 8/8,7/8,6/8,5/8,4/8 and 0,group B were 7/8,5/8,5/8,4/8,3/8 and 0,group C were 3/8,2/8,2/8,1/8,0 and 0;all mice in control group were zero. At all time points, there were statistical difference in the positive rate among three experimental groups only at 1 day (P<0.05 ). (2) In the positive mice, their UU3 quantity concentration at 1,3,7,14,21 days were 1.70×107, 8.26×106, 4.04×106, 2.86×106,and 2.41 x105 copy/g in group A; 3.75×106, 2.56×106 , 1.37×106, 6.72×105, and 1.12 x 105 copy/g in group B, and 1.45×105,1.07×105, 5.43×104, 4.68×103, and 0 copy/g in group C. There were statistical difference among experimental groups at all time points except 21 days (P<0.05). Comparing the concentration among all time points of every group, both group A and B showed a significant difference(P<0.05) ,group C didn't reach it( P>0.05). (3) The total pathogenicity rates of three groups were significant different at 7-35 days, which were 56% (18/32) in group A, 44% (14/32) in group B, 6% (2/32) in group C (P<0.01 ). And at 7,14,21,35 days of postineculation, the pathogenicity rates in group A were 5/8,5/8,4/8 and 4/8, group B were 4/8,4/8,3/8 and 3/8, group C were 1/8,0,1/8 and 0; all mice in control group were zero, which demonstrated significant difference only at 14 days (P<0.05), no other statistical difference were observed (P>0.05) . Conclusions The pathogenicity of UU3 varies with different concentration in genital tract of mice. When UU3 concentration is more than 1×106 copy/g, the susceptibility to infection was intensified significantly.

Objective To study the kinetic expression level of chemokines (RANTESF) in the murine infection of vulvovaginal can-didiasis (VVC), and explore the function of chemokines in local immunity of VVC. Method Sixty-three female Kunming mice, at 8 ～ 10 weeks of age, were used in this study. All animals were divided into three groups. The content variation of RANTESF in blood and yaginal fluids and CFU of vaginal fluid in each separate group of mice were detected at days 2, 7, and 14 after infection. The first group was control group. The second group was infected only one time and the third group was infected twice. The results were analyzed with SPSS 13.0 statis-tical software. Results The content variation of RANTESF and CFU in vaginal fluid reached highest at days 7 in both the first and the sec-ond groups, as well as in the blood. There were no notable changes at days 2 and 14. The content variation in vaginal fluid or blood of the second group was higher than that in the first group after infection. Conclusion CMI, as a host defense mechanism, plays an important role in protecting against vulvovaginal candidiasis, especially in secondary infection. Local innate immunity is more important than systemic in-nate immunity for protection against vulvovaginal candidiasis. Cytokine about RANTES can promote innate immunity modulation; especially the local innate immunity modulation can promote the ehemotaxis of RANTES.

Purpose To investigate the clinical pathological features, pathological diagnosis and differential diagnosis of the non-Wilms’ renal tumors in children. Methods The clinical pathological data and immunohistochemical findings of 15 cases of non-Wilms’ renal tumors were retrospectively analyzed with review of the relevant literature. Results In 7/15 (46. 67%) cases of cystic nephroma ( CN) , microscopically the variable size of cysts were lined by a single layer flattened, cuboidal epithelia, separated by fi-broblastic stroma;in 4/15 (26. 67%) cases of rhabdoid tumor of kidney (RTK), histological characteristics were rhabdoid cells and cytoplasmic inclusions;in 2/15 (13. 33%) cases of clear cell sarcoma (CCSK), the classical pattern of histology demonstrated nests, cords of ovoid epithelioid or spindled clear cells separated by fibrovascular septa, which had a marked ’ chicken wire’ pattern of small blood vessels;in 1/15 (6. 67%) case of congenital mesoblastic nephroma (CMN), the tumor was mainly composed of spindle cells arranged in fascicular pattern, in which fetal renal tissue was seen;in 1/15 (6. 67%) case of granularity renal cell carcinoma, micro-scopically, the cancer cells with eosinophilic granular cytoplasm arranged in nests, cords and tubular pattern. Conclusions Although non-Wilms’ renal tumors is a small part of all kidney tumor, it is difficult to diagnose and distinguish from other tumors before opera-tion. The pathological diagnosis is the most reliable method for differential diagnosis of non-Wilms’ renal tumor.

Objective To explore the influence of human resources management in emergency department on nurse's anxiety.Methods Self-Rating Anxiety Scale(SAS)was used to investigate 300 nurses in the emergency department in nine secondary level hospitals of Longgang District.Division of human resource management in these hospitals were examined and the correlation between them was statistically analyzed.Results In addition to the three hospitals,nurses anxiety status of the other six hospitals score showed different levels of anxiety,the average scores were above 50,but the hospitals,that the ratio of the three anxiety condition score was less than 50,moderate to severe anxiety were still more than 20.0％,and other six hospitals was up to 66.7％.In the human resource management to achieve excellent and qualified hospital,the anxiety rate was significantly lower than the substandard hospitals.At the same time there was close correlation between the situation of human resource management and nurses anxiety situation.Conclusions Emergency nurses generally have high anxiety,and in hospitals with poor human resource management,career anxiety is even more obvious.That is to say the anxiety of nurses is closely related to emergency department management of human resources.

Objective To explore the clinical manifestations, electroencephalographic characteristics and therapeutic effect of drugs in children with Jeavons syndrome. Methods The clinical and electroencephalographic characteristics and thera-peutic effect of drugs were analyzed in 4 children with Jeavons syndrome. Results Among the four children there were 3 female and 1 male. The age at the onset of the disease was from 1 to 6 years. The typical clinical manifestations of this disease were brief, fast and repeated eyelid myoclonia (EM) with or without absence seizure. The typical electroencephalography (EEG) in two patients showed 3-6 Hz generalized spike and waves and polyspikes burst, and the eye closure and intermittent photic stimu-lation helped to induce discharges and clinical events. The typictal EEG in the other two patients showed 3.0-3.5 Hz generalizedδslow wave rhythm burst. The drugs of choice for treatment was sodium valproate monotherapy in two cases, levetiracetam in one case, sodium valproate combined with levetiracetam in one case. During the follow-up, seizures were controlled in one case, decreased in frequency in two cases and were still frequent in one case. Conclusions Jeavons syndrome is one of the idiopathic and generalized epileptic syndromes and characterized by EM with or without absence seizure. Video EEG monitoring plays an important role in the diagnosis of this disease. Sodium valproate and levetiracetam were effective for this disease.

Objective To explore the effect of telomerase reverse transcriptase(TERT) gene transfected bone marrow stem cell(BMSC)on the memory function and hippocampal CA1 region synaptic plasticity in vascular dementia rat.Methods A total of 60 rats were randomly divided into the negative control group(group A),model group(group B),conventional BMSC group(group C) and transfected BMSC group(group D).The related indicators in each group were detected by using the Morris maze test,RTPCR and Western blot respectively.Results The escape latency period in the group C and group D was significantly longer than that in the group B,which in the group D was significantly longer than that in the group C.Compared with the group A,the expressions of brain-derived neurotrophic factor(BDNF)mRNA,TERT mRNA,SYP mRNA and protein in the group B,group C and group D were significantly decreased.The synaptic cleft arrange in group A was clear with more SYN positive ceils.The synaptic cleft in the group D was clearer,and the number of SYN positive cells was close to that in group A.Conclusion TERT transfected BMSC has obvious therapeutic effect on vascular dementia rats and its mechanism may be related to the promotion of BDNF,TrkB expression and the improvement of synaptic plasticity.

Objective To compare the effects of moderate and deep hypothermic circulatory arrest (DHCA) during aortic arch surgery in the adult patients,to offer the evidence for the detection of which temperature provides best brain protection in the subjects who accept the great aortic surgery.Methods A total of 109 patients undergoing the surgery of aortic arch were divided into the moderate hypothermic circulatory arrest group and deep hypothermic circulatory arrest group.We recorded the characters of the patients and their cardiopulmonary bypass time,aortic clamping time,cerebral perfusion time and postoperative recovery time,tracheal intubation time,time of intensive care unit (ICU) and postoperative cognitive dysfunction.Results Patients' characteristics were similar in two groups.All the patients were cured.There were no significant differences in aortic clamping time of each group [(111.4 ± 58.4) min vs.(115.9 ± 16.2) min];selective cerebral perfusion time [(27.4 ± 5.9) min vs.(23.5 ±6.1) min] of the moderate hypothermic circulatory arrest group and deep hypothermic circulatory arrest group.There were significant differences in the cardiopulmonary bypass time[(207.4 ± 20.9) min vs.(263.8 ± 22.6) min],the postoperative recovery time [(19.0 ± 11.1) h vs.(36.8 ± 25.3) h],intubation time [(46.4 ± 15.1) h vs.(64.4 ± 6.0)h];length of ICU [(4.7 ± 1.7) d vs.(8.± 2.3) d],and postoperative cognitive dysfunction of the two groups.Conclusion Compared to the deep hypothermic circulatory arrest,the moderate hypothermic circulatory arrest can provide better brain protection and achieve good clinical results.