Objective To explore the causes of perioperative anxiety and depression among patients with ovarian cancer. Methods With phenomenological method of gicalitative research,10 patients with ovarian cancer were interviewed and the data were analyzed with content analysis.Results The causes of perioperative anxiety and depression among the young patients with ovarian cancer included psychological barrier,care and self-accusation about their family; changed marital relationship,worries about increased economic burden on families; reduced sense of self-worthiness.Conclusion Nurses should know and master the causes of their anxiety and depression,pertinently work out nursing measures to eliminate the anxiety and depression and promote patient’s active cooperation with treatment so as to improve curative treatment and quality of life.

Objective To explore the effect of strong concept on the postoperative personality and quality of life of patients with esophageal cancer. Methods One hundred esophageal cancer patients during June to December 2014 were set as controls, and another 100 esophageal cancer patients during January to June 2015 as the observation group. The latter were intervened by strong concept including strong cognitive adjustment, belief adjustment and behavioral modification. Eysenck Personality Questionnaire and esophageal cancer QOL were used for evaluation. Results After the intervention, the scores on neuroticism, psychoticism and lie scale in the observation group were lower than those in the control group as well as pre-intervention , so it was with the scores on intrinsic and extrinsic scales. The quality of life in the observation group was significantly higher than that of the control group and before intervention (P<0.05). Conclusion Strong concept used for intervention can improve the personality of postoperative personality and quality of life of esophageal cancer patients by helping them to be confident and optimistic to face life positively.

Objective To evaluate the efficiency of lamivudine combined with hepatitis B immunoglobulin to prevent hepatitis B recurrence after liver transplantation.Methods The literature concerning the application of lamivudine and hepatitis B immunoglobulin after liver transplantation was collected.The efficacy of initial lamivudine,and hepatitis B immunoglobulin alone or combined together was evaluated in liver transplantation recipients with hepatitis B by performing a systematic review of the literature with a Meta-analysis of clinical trials.Odds ratio(OR)was applied to evaluate the effect of therapeutic alliance to decrease the reinfection rate whether or not.Results We identified 7 clinical trials,and there were 360 patients subjected.OR and 95% confidence interval(95%CI)was 0.34(95%CI ranging from 0.18 to 0.64).For overall test result,Z value was 3.33 and P value was 0.01.The P value was 0.310 for our test of study homogeneity.Conclusion Our meta-analysis shows that lamivudine or hepatis B immunoglobulin can effective prevent hepatitis B recurrence after liver transplantation,and therapeutic alliance is more effective than monotherapy,and tolerance to lamivudine or hepatis B immunoglobulin was good.

Objective To explore the relationship between dietary patterns and non alcoholic fatty liver disease (NAFLD) in Karamay adults.Methods A total of 2377 subjects aged 20-75 years old who took health check-up during April and June 2011 were included in this investigation.Body mass index (BMI),waist to hip ratio (WHR),systolic blood pressure (SBP),diastolic blood pressure (DBP),triglyceride (TG),total cholesterol (TC),aspartate aminotransferase (AST) and serum uric acid (SUA)were measured.Food intake patterns and life style of the participants were accessed by using food frequency questionnaire (FFQ).Main component analysis and multivariate logistic regression model were used to explore the association of dietary patterns with NAFLD.Results Five dietary patterns were identified,including condiment dietary pattern, western dietary pattern, conventional healthy dietary pattern,snacks and bean pattern,and high protein dietary pattern.Subjects of condiment dietary pattern,western dietary pattern or conventional healthy dietary pattern showed significantly different prevalence of NAFLD (x2 values were 149.873,8.247 and 18.766,respectively; all P ＜ 0.05).In condiment dietary pattern,age,BMI,WHR,SUA and TG were significantly different in Q4 group (F values were 8.42,5.64,12.78,10.72 and 9.63,respectively; all P ＜0.05).For those with western dietary pattern,age,WHR,SBP,SUA,TC and TG showed significant different trend (F values were 9.84,6.87,6.11,10.61,6.87 and 8.13,respectively; all P ＜ 0.05).However,subjects with conventional healthy dietary pattern showed a significant different trend of age,BMI,WHR,SBP,SUA and AST (F values were 19.13,11.09,13.89,6.11,15.12,6.19,respectively ; all P ＜ 0.05).After age,BMI,WHR,SBP,SUA,AST,TC and TG were adjusted,the risk of NAFLD of those with condiment pattern or western pattern were significantly increased (odds ratio (OR) were 1.33 (95％ confidence interval (CI) 0.95-1.87) and 1.36 (95％ CI 0.979-1.902),respectively).Conclusion Dietary pattern may be associated with NAFLD.People need to learn healthy dietary habit to prevent NAFLD.

Objective To observe the expressions of OPN, COX-2 and CyclinD1 in breast infiltrating carcinoma and evaluate their relationships with clinic pathological features. Methods Expression of the above three indexes were detected from 70 breast cancinoma patients by immunohistochemistry. The relationships among them and clinicopathological features were analyzed. Results The positive expression rates of OPN were 78.8% in cases (≤45 years old) and 73.0% in cases (> 45 years old); the positive expression rates were 79.3%(tumor diameter ≤ 3 cm) and 73.2% (tumor diameter > 3 cm); the positive expression rates were 77.8%, 73.8% and 78.9% in cases ofⅠgrade, Ⅱgrade and Ⅲ respectively, the positive rates had no statistical significances(P > 0.05). The expression rates of OPN in cases of breast infiltrating carcinoma without and with axillary node metastasis were 62.5% and 93.3%, in cases at stage Ⅰ~Ⅱ and Ⅲ ~Ⅳ were, 68.0% and 95.0% respectively, the positive rates had statistical significances(P < 0.05). The expression of OPN was negatively correlated with ER and PR while positively correlated with CerbB2, COX-2 and CyclinD1. Conclusions OPN plays an important role in the invasion and metastasis of breast carcinoma coordinated with COX-2 and CyclinD1.

0.05)in the main pharmacokinetic parameters between the domestic preparation and the imported preparation,which suggests they are bioequivalent.

OBJECTIVE:To study the pharmacokinetics of prulifloxacin capsules in Chinese healthy volunteers after single and multiple oral administration of prulifloxacin capsules.METHODS:A total of 12 healthy adult subjects were randomly grouped by 3? 3 Latin square,who were assigned to receive oral single dose of 132,264 and 528mg prulifloxacin capsules and multiple doses of 264mg prulifloxacin capsule for 6 days in succession.The blood concentration of NM394-the metabolite of Prulifloxacin was determined by HPLC at different time after oral administration of Prulifloxacin.The simulation and fitting,and computation of parameters were performed using DAS ver1.0 software.RESULTS:All 12 subjects had completed single oral administration test,with no adverse drug reactions appeared during the test.No prulifloxacin but its metabolite-NM394 was identified in the blood sample of subjects.The high,medium and low dosage groups were all fitted two-compartment model.The pharmacokinetics fitted first order kinetics process without gender difference.There was no accumulation and pharmacokinetic parameters change after multiple oral administration of prulifloxacin,suggesting prulifloxacin had no self-enzyme inhibition or induction.CONCLUSION:The established method is sensitive,accurate,reliable and specific,and it can meet the requirement of clinical pharmacokinetic trial.Its parameters are in line with literature reported abroad,with no gender difference among Chinese adults.

Objective To determine the pharmacokinetic interaction between cefalor and bromhexine in healthy Chinese volunteers. Methods Twelve subjects received a cefaclor (CEF) treatment, a bromhexine (BHX) treatment, and a co-treatment of CEF and BHX with a 3 × 3 Latin square design. The wash-out time between periods was 14 days. The plasma and urine drug concentrations of CEF and BHX were detected by HPLC-UV and LC/MS, respectively. Results All the 12 volunteers completed the study. There were no significant differences in AUC0-t and Cmax of CEF in logarithm between the single administration group of CEF and the co-administration group of CEF with BHX. Two one sided t-test showed that CEF was bioequivalent in the 2 groups. There were no significant differences in tmax, MRT, t1/2, and Clr between the 2 groups. Vd/F was significantly lower in the single CEF group than in the co-administration group of CEF and BHX. There were no significant differences of AUC0-t and Cmax of BHX in logarithm between the single administration group of BHX and the co-administration group of BHX with CEF. Two one sided t-test showed that BHX was bioequivalent in the 2 groups. There were no significant differences in tmax, MRT, t1/2, Vd/F, and Clr between the 2 groups. Conclusion There is no significant pharmacokinetic parameter change in the drug absorption, metabolism, and excretion, but Va/F of CEF significant increases in the co-administration of CEF with BHX. The co-administration of CEF and BHX has no adverse drug interaction. The increase of Vd/F may be a favorable drug interaction, which may be the mechanism of the synergistic effect of the 2 drugs.

Objective To investigate the induction of B-cell specific phenotype in classical Hodgkin lymphoma (cHL)upon all-trans retinoic acid(ATRA)incubation. Methods To construct B-cell specific promoter(CD19, CD79a,CD79b)driven reporter plasmid with NEO cassette to realize stable transfection and selection of cHL reporter cells. To verify the intact integration by amplification of the promoter and luciferase sequences,and to functionally validate the B-cell specific promoter by ABF1 interference and luciferase assay. Repoter cells were incubated with various doses of ATRA and luciferase activity was detected at 24,48 and 72 hours. Reporter cells were treated alone or in combination with 5-Aza and ATRA followed by luciferase assay. Endogenous B-cell specific genes(CD19, CD20, CD79a and CD79b) transcription and expression levels were detected by real-time PCR and immunoblot, respectively. The expression level of CD30 antigen on Hodgkin lymphoma cell membrane upon ATRA was assessed by flow cytometry. Results ATRA treatment stimulated B-cell specific signature in cHL cells including CD19,CD79a and CD79b while down-regulated their CD30 expression. Conclusions ATRA induces B-cell phenotype deficient cHL cells to regain their B-cell transcriptional program while abolishes their Hodgkin-specific machinery.

OBJECTIVE: To determine the effect of sperm morphology on fertilization rate, cleavage rate,and clinical pregnancy rate, which may help to choose a best protocol before assisted reproductive technology (ART). METHODS: We a retrospectively surveyed semen data of 1369 ART patients from January to December 2008 in our hospital. We divided sperms from 751 patients into 4 groups based on the sperm morphology rate: ≤5.0% (normal), 5.1%-10.0%, 10.1%-15.0%, and >15.0%. In vitro fertilization (IVF) was used for 490 patients or intracytoplasmic sperm injection (ICSI) for 261. We also divided sperms from 618 donors into 4 groups based on the sperm morphology rate: 14.0%-17.0%(normal), 17.1%-19.0%, 19.1%-23.0%, and >23.0%. Only IVF was used for these donors. The fertilization rate, cleavage rate, and clinical pregnancy rate among each group were analyzed and discussed. RESULTS: During the IVF(patients or donors) cycle, there was a significant difference among the 4 groups in the fertilization rate and clinical pregnancy rate (P<0.05), but there was no difference in the cleavage rate (P>0.05). In the ICSI cycle, there was no significant difference among the 4 groups in the fertilization rate, clinical pregnancy rate, and cleavage rate (P>0.05). CONCLUSION Sperm morphology affects the outcome of IVF. There is no significant influence of sperm morphology on the outcome of ICSI. ICSI is an optimal alternative for patients with abnormal morphological sperms in ART.
Adult ; Female ; Fertilization in Vitro ; Humans ; Infertility, Male ; therapy ; Male ; Pregnancy ; Pregnancy Rate ; Reproductive Techniques, Assisted ; Retrospective Studies ; Sperm Injections, Intracytoplasmic ; Spermatozoa ; cytology