Objective To investigate the role of eotaxin (ehemotacfie factor of eosinophils) in pathogenesis of eosinophilie airway inflammation and airflow obstruction. Methods 45 eases of patients in exacerbations with bronchial asthma (A group) and 25 eases of healthy volunteers as controls (B group) have participated in lung func-tion test and sputum induefion. The concentration of eotaxin was measured with sandwich enzyme-linked immunosor-bent assay (ELISA). Results The differences of the eosinophil (EOS)/leukoeyte (Leu)% in the two groups are significant (P <0.05) ; the differences of the eotaxin in the two groups is also significant (P < 0.05) ; the EOS/Leu% and the level of eotaxin are significantly inversely correlated with the percentage of the first second forced ex-piratory volume/predicted value (FEV1%) (P < 0.001). The level of eotaxin in induced sputum is significantly positively correlated with the EOS/Leu% in A group (P<0.001). Conclusion Eotaxin poasiblely partieulatesin e-osinophilic airway inflammation and airflow obstruction in asthmatic patients by selective chemotactic activity on eosin-ophil, and eosinophil release a series of inflammation medium that induce the exacerbation of asthma.

The total effective rate of Xiaoying Plaster applied top-ically for dissipating or reducing the size of the masswas 84.34% while that of the control group was 42.9%.Animal experiment also revealed that local appli-cation of this plaster alone can markedly reduce thethyroid mass,restoring the diffuse proliferation of thy-roid tissue to normal.The antigoiter effect is similar tothat of oral administration of thyroid tablet.

Objective To study the effectiveness of Suhuangzhike Capsule on eosinophilia (Eos) and exhaled nitric oxide (FeNO) in patients with cough variant asthmathe.Methods 98 patients with cough variant asthmathe were randomly divided into the two groups,the control group (n =49 cases) and the observation group (n =49 cases).The patients in the control group were treated through the conventional treatment,while the patients in the observation group were treated through the conventional treatment plus Suhuangzhike Capsule.They were treated for four weeks.Eos and FeNO were detected before and after treatment.Results The total effective rate of the control group was 87.8％,which of observation group was 95.9％;The total effective rate of the observation group was obviously higher than that of the control group (x2 =4.86,P ＜ 0.05).The two groups after treatment the blood Eos and FeNO were significantly lowered (t =5.102,8.772,7.921,10.224,P ＜ 0.05,P ＜ 0.01).And compared with the control group after treatment,the patients in the observation group decreased more significantly (t =2.714,3.336,all P ＜ 0.05).Conclusion Suhuangzhike Capsule can decrease eosinophilia and exhaled nitric oxide in patients with cough variant asthmathe.

It is increasingly evident that sepsis triggers a complex host reaction, which is responsible for a variety of pathophysiologic changes during the inflammatory process. Pentoxifylline (PTX) is a methyxanthine with selective anti inflammatory activity. The effects and mechanisms of PTX in human and animal models of sepsis are reviewed, for providing new information in treatment of sepsis.

Objective To investigate the effect of cisplatin and 5-fluorouracil on endocrine Ishikawa cells in the treatment of endometrial cancer. Methods The human endometrial cancer cell line Ishikawa were selected, at the logarithmic growth period, the cells were divided into three groups of 5-fluorouracil (5 mg/L) group, cisplatin (5 mg/L) group and cisplatin (5 mg/L) combined with fluorouracil (5 mg/L) group ( combined group).After treated with corresponding drugs treatment, the cell viability was detected by MTT, the apoptosis was detected by flow cytometry, and the Bcl-2 and p65 expressions cells were detected by Western blot.Results The inhibitory rate of combined group, cisplatin group and 5-fluorouracil group were (41.45 ± 3.13)%, (25.20 ±3.09)% and (23.19 ±4.10)% respectively, and the inhibitory rate in the combined group was significantly higher than that of the other two groups (P<0.05).The apoptosis rate of the combined group, cisplatin group and 5-group were (29.44 ±4.35)%, (5.74 ±1.12)% and (5.82 ±1.78)% respectively, and the apoptosis rate in the combined group was significantly higher than that of the other two groups (P<0.05).The Bcl-2 relative expression in the combined group, cisplatin group and 5-fluorouracil group were (0.31 ±0.11), (1.23 ±0.49) and (1.28 ±0.59), the p65 expression were (0.67 ±0.23), (1.67 ±0.56) and (1.71 ±0.71), combined group of Bcl-2 and p65 relative expressions were obviously less than that of the other two groups ( P <0.05 ) .Conclusion Cisplatin combined with 5-fluorouracil on endocrine Ishikawa cells in the treatment of endometrial cancer could promote cell apoptosis, inhibit the cell proliferation, and its mechanism may be related to the inhibition of Bcl-2 and p65 protein expressions.

Objective:To investigate the influence of lant ha num chloride on the proliferation of human gingival fibroblasts(HGFs).Me thods:HGFs were cultured in vitro and MTT assay was used to analyse the influence of lanthanum chloride at 10 -6,10 -5,5?10 -5,1 0 -4,10 -3 and 10 -2 mol/L respectively on the proliferation o f HGFs. Results:Lanthanum chloride at 1?10 -2 mol/L inhib ited the proliferation of HGFs,however, it did not at 10 -6~10 -3 mo l/L. Conclusion:Lanthanum chloride at a higher concentration(1 ?10 -2 mol/L)may inhibit HGF proliferation.

Objective To investigate the role of central and peripheral sensitization in remifentanil-induced hyperalgesia in a rat model of inflammatory pain. Methods Twenty-one male SD rats weighing 200-300 g were used in this study. Inflammatory pain was induced by intraplantar injection of 1 % carrageenan 100 fd in the left hindpaw in all animals. The animals were then randomly divided into 3 groups ( n = 7 each): control group (group C) and two remifentanil groups (group R1 , R2) . In R1 and R2 groups remifentanil was infused iv at a rate of 10 and 30 μg-kg-1·min-2 respectively starting from 5 min before till 25 min after carrageenan injection, while in group C normal saline was infused iv instead of remifentanil. Bilateral paw withdrawal threshold to mechanical stimulation with von Frey filament (PWT) was measured before (baseline) and at 1 h, 3 h and 1-7 d after carrageenan injection. Bilateral paw withdrawal latency to noxious thermal stimuli (PWL) was measured before and at 2 h, 4 h and 1-7 d after carrageenan injection. The thickness of the plantar surface of left hindpaw was measured before and at 1 h, 4 h and 1-7 d after carrageenan injection. Results Bilateral PWT was significantly lower at day 1 after carrageenan injection in R, and R2 groups than in group C. The right PWT was significantly lower at 2 d and 4-7 d after carrageenan injection in group R2 than in group R, . There was no significant difference in PWL and thickness of the plantar surface of left hindpaw among the 3 groups. Conclusion Central sensitization is involved in developing and maintaining the remifentanil-induced hyperalgesia in a rat model of inflammatory pain, while peripheral sensitization is not.

A kind of magnesium potassium phosphate cement developed for bone repair was cleared by FDA in 2009 and has been presently in clinical use in America.The biomaterial has the powerful adhesive capability to bind bone,ligament,and tendon to bone,as well as possessing good biocompatiblity,appropriate biodegradability and osteogenicity; to data,it is the only material which possesses the combination of adhesivity and osteogenicity among bone repair biomaterials.The clinical application of the innovative biomaterial will unprecedentedly alter the treatment in orthopedic surgery and related disciplines.To provide a comprehensive appreciation of the innovative biomaterial,this article summaries its development,characteristics of composition and preparation,formation mechanism,application study and superiority.

Objective To study the effect of glucosides of cbaenomeles speciosa (GCS) on the expression of EP2 and EP4 in synovial tissues from rats with adjuvant arthritis. Methods Complete Freund's adjuvant (FCA) was used to induce adjuvant arthritis (AA) in rats. The synoviocytes from AA rats were collected and incubated. Synoviocytes proliferation was determined by 3-(4,5-dimethylthiazal-2yl) 2,5-dipbeny+hetrazoliumbromide (MTT) assay. Levels of prostaglandins E2(PGE2) and cAMP were determined by radioim-munoassay (RIA). The expression of EP2 and EP4 was detected by Western blot analysis. Results GCS significantly inhibited the proliferation of synoviocytes. GCS decreased the level of PGE2 and increased the level of cAMP. The results of correlation analysis demonstrated that GCS inhibited synoviocytes hyperplasia by increasing the level of cAMP in synoviocytes. The results of Western blot analysis demonstrated that GCS significantly improved the expression of EP2 and EP4. Condusion These results suggest that restoring normal transduction of the EP-G protein-cAMP signal pathway may be one of the most important mechanisms of GCS.

Objective To explore the treatment effect of human growth factor (NGF) on vascular dementia. Method 47 cases with vascular dementia were randomly divided into two groups (study and control). The study group (24 cases) were treated with NGF, the control group (23 cases) were treated with ordinary medicines.Results The intelligence and cognitive ability in the study group were obviously higher than those in the control group (P<0.01). Conclusion Compared with other methods, NGF has a significant curative effect.