In the present study it was observed that the reactivity of splenocytes to Con A,LPS and IL-2 was enhanced in the mice which were given Total Soyasaponin(Ts)Via mouth?We also found that the level of IL—2 in splenocytes and the activity of NK and LAK cells were obviously higher than that in the control group.

Objective To evaluate the reliability and validity of the Chinese version of job satisfaction scale in order to supply quantitative tool for evaluation of nurses" job satisfaction degree. Methods 336 nurs-es from 14 general hospitals were tested with Chinese version of job satisfaction scale and analysis was carried out for reliability and validity according to the test result. Results The total Cronbach's α coefficient of Chi-nese version of job satisfaction scale was 0.901, Cronbach's α coefficient range of every dimension was 0.732～ 0.921, re-test reliability was 0.832, 9 factors were extracted through factors analysis and they explained 59.57%of the overall variance. Conclusions Chinese version of job satisfaction scale has good reliability and validi-ty, it can be used in evaluation of nurses' satisfaction degree preliminarily.

Objective To observe the effect of Succinate injection combined with Bifico on the myocardial enzymes changes of the children with rotavirus enteritis.Methods A total of 146 children with rotavirus enteritis were enrolled and included in this study. Children were randomly divided into the control group (n=73) and the observation group (n=73). The control group was received Bifico on the basis of routine treatment, and observation group was received Succinate injection combined with Bifico. The IL-17, IL-6, TNF-?, LDH, CK, and CKMB was detected by ELISA. The rates of clinical effects was compared.Results After treatment, the IL-17 (22.35 ± 4.21 ng/mlvs. 30.24 ± 6.07 ng/ml,t=2.395), IL-6(31.26 ± 6.14 ng/mlvs. 43.72 ± 8.22 ng/ml,t=2.347), TNF-? (35.62 ± 6.24 ng/mlvs. 49.18 ± 8.72 ng/ml,t=2.421), LDH (135.16 ± 31.25 U/Lvs. 174.08 ± 40.22 U/L,t=2.373), CK (37.82 ± 7.39 U/Lvs. 50.21 ± 11.16 U/L,t=2.385), and CKMB (90.14 ± 11.63 U/Lvs. 113.22 ± 18.35 U/L,t=2.392) in the observation group were lower than those in the control group (P<0.05). The rates of clinical effects was 94.5% (69/73) in the observation group and 83.6% (61/73) in the control group. There was significant difference between the two groups (χ2=2.352,P=0.047).Conclusions The Succinate injection combined with Bifico could reduce the inflammatory indices and alleviate the myocardial injury in the RVE patients.

Objective To explore the inhibition effect of glutamine on the inflammation of rats with pneumonia induced by Klebsiella pneumoniae.Methods Thirty-six SD rats were randomly divided into the normal group,model group and glutamine treatment group.The rat pneumonia model was established by intratracheal instillation of Klebsiella Bacillus pneumonia.Results Compared with the model group,the glutamine treatmernt group could reduce the ratio of W/D(5.98±0.29)vs.(4.32±0.33)(P＜ 0.05),alleviated the lung tissue edema,inflammatory cell infiltration and then improved the morphology of the lung tissue,inhibited the IL-6,IL-1 and TNF-α secretion in serum and lung homogenate(P＜0.05),and inhibited the level of NF-κB p65 and IκBα phosphorylation.Conclusion Glutamine inhibits the inflammation of rats with pneumonia induced by Klebsiella pneumoniae,which might be related to NF-κB signal pathway.

Objective To study the relationship between juvenile transient synovitis of the hip and enterovirus.Methods EV-IgM antibody was detected with ELISA in blood and arthrocentesis. At same time, sample with coxsackievirus B(CVB) IgM positive was tested by serptype.Results IgM antibody was detected in 38.27% in blood of the patients and in 76.19% in arthrocentesis.CVB-IgM was found positive in 16.05% of blood and in 42.86% of arthrocentesis.AdV-IgM was in 11.73% and in 23.81%,respectively.Most of the CVB-IgM positive individuals were infected with CVB3.Conclusion The results of this study suggest that there may be a significant correlation between juvenile transient synovitis and CVB, especially CVB3.

Objective The aim of this study is to undemtand the development of peripheral nerve injury on diabetes mellitus.Methods Diabetes was induced by STZ.We determined nerve conduction velocity,thermal pain threshold,morphoh)gy changes of sciatic nerve and quantitative analysis of sural nerve of diabetic model group and normal control group after 2 weeks,4 weeks,6 weeks,8 weeks,12 weeks respectively,and observed dynamically functional and morphologic changes of peripheral nerve of diabetic rats.Results After 2 weeks,diabetic rats showed that motor and sensory nerve conduction velocities slowed down and thermal pain threshold decreased.After 4 weeks.changes of pathology of sciatic nerve were observed.With the duration extending,the damages of nerve were aggravated gradually.Conclusions Neumpathy appeared in earlier stage on experimental diabetic rats and functional changes were preceded to morphologic changes.

Using inverted microscopy and circuit TV, the morphological type, diameter of arteriovenous anastomoses (AVAs) in 11 rabbit's ear chambers were observed. According the joining forms with arterioles and venules in 68 AVAs, there were 4 types: type Ⅰ 58.8％, type Ⅱ 19.4％, type Ⅲ 11.1％, type ⅣV 10.7％. The inner diameters of the AVAs when dialated, measured 36.6?1.67?m at their arterial ends, 16+0.6?m at their narrow points, 66?4?m at their venous ends.This study showed that dilation of AVAs in the rabbit's ear is a graded response which correlates with the rise in intravertebral canal temperature. Some AVAs have multiple venous segments which vary in diameters. It was observed that the behaviour were different after intravertebral canal warming.

To monitor the 24 h esophageal pH in patients with gastroesophageal reflux-induced cough (GERC) before and after omeprazole treatment. Methods The prospective study was conducted in Department of Gastroenterology, People's Hospital of Wuhan University from February 2007 to April 2008. Twenty patients with GERC received 40 mg of omeprazole daily and underwent 24 h esophageal pH monitoring before and 8 weeks after omeprazole treatment. Six parameters by Johson and Demeester were adopted for evaluation: pereentage of total time with pH <4, percentage of uptight reflux time with pH <4, percentage of supine reflux time with pH < 4, the number of reflux episodes with pH < 4, the number of reflux episodes longer than 5 min and the percentage of longest reflux duration. Results The values of six parameters before treatment were 13.5±8.5, 12.2±4.0, 15.8 ±4.1, 56 ± 13, 4. 15 ± 1.87 and 26.2 ± 16. 5 respectively; while those after treatment were 2.7± 1.3, 3.9 ± 1.9, 1.9 ± 1.0, 18 ± 11, 1.24 ±0.65 and 7.4 ± 2. 1. There were significant differences in these six parameters between pre-treatment and post-treatment (P<0.01 ). Conclusion The 24 h esophageal pH monitoring shows that omeprazole is effective in treatment of GERC by decreasing esophageal pH.

Objective To investigate the effects of angiotensin-converting enzyme inhibitor (ACEI) lisinopril on experimental diabetic peripheral neuropathy (DPN) in the earlier stage and its mechanism. Methods Diabetes was induced by streptozotosin (STZ) in rats. After 8 weeks of treatment, the effects of lisinopril on function and structure of peripheral nerves were observed; superoxide dismutase, malondialdehyde, Na+K+-ATPase activity in sciatic nerve and NO, NO synthase levels in plasma were determined, and capillary density of sciatic nerve were also assessed. Results With lisinopril, nerve structure and function was improved, oxidative stress state was ameliorated, and NO level, Na+K+-ATPase activity and capillary density of endoneurium were increased (all P<0.05). Conclusion ACEIs are effective in DPN. The possible mechanisms may be related to the amelioration of ischemic state and related metabolic disorders of the nerves.