Objective To investigate the efficacy and safety of HCPT combined with 5-fluorouracil(5-Fu) in treatment of bladder cancer .Methods Selected 92 patients with advanced bladder cancer in our hospi-tal from February 2011 to August 2015 , equally divided into observation group and control group of 46 patients based on the random number table ,the control group were received 5-Fu intravesical chemotherapy ,The obser-vation group were given the HCPT combined with 5-Fu chemotherapy ,the chemotherapy infusion were once a week,continuous perfusion were observed for 3 months.Results The response rates in the observation group was 95.7%and the control group was 76.1%,the observation group were significantly higher than the control group (P<0.05).The occurrence of bladder irritation adverse reaction ,blood abnormalities,liver and kidney dysfunc-tion,bone marrow suppression in the observation group during chemotherapy compared with the control group were no significant difference(P>0.05),all adverse reactions after symptomatic treatment were improved .All patients were followed up for 1 year,The mortality and recurrence rates in the observation group were 8.7%and 4.3%re-spectively,while the control group were 30.4%and 17.4%,the mortality and recurrence rates of the observation group were significantly lower than the control group (P<0.05).Conclusion HCPT combined with 5 -Fu chemotherapy for bladder cancer has strong anti -cancer effect and without increase the incidence of adverse re-actions,it can promote the improvement of the long -term prognosis,so it is a safe and effective topical bladder chemotherapy drugs .

Objective To evaluate the reliability and validity of the Chinese version of job satisfaction scale in order to supply quantitative tool for evaluation of nurses" job satisfaction degree. Methods 336 nurs-es from 14 general hospitals were tested with Chinese version of job satisfaction scale and analysis was carried out for reliability and validity according to the test result. Results The total Cronbach's α coefficient of Chi-nese version of job satisfaction scale was 0.901, Cronbach's α coefficient range of every dimension was 0.732～ 0.921, re-test reliability was 0.832, 9 factors were extracted through factors analysis and they explained 59.57%of the overall variance. Conclusions Chinese version of job satisfaction scale has good reliability and validi-ty, it can be used in evaluation of nurses' satisfaction degree preliminarily.

Objective To investigate the role of P21 protein and XIAP on apoptosis-inducing effect by mevastatin against A549 cell line and its mechanisms. Methods The cells proliferation was detected by MTT assay. The cell cycle distribution and apoptosis induction were evaluated with flow cytometer and electron microscopy. The mRNA expression of P21 and XIAP was measured with reverse transcription-polymerase chain reaction.The protein expression of P21 and XIAP was tested with flow cytometer and Western Bolt respectively. Results Mevastatin inhibited A549 cell survival in a time-dependent and concentration-depended manner. Flow cytometry showed that mevastatin induced G0/G1 phase arrest and caused apoptosis. Mevastatin did not affect P21 expression at both mRNA and total protein level.Concomitantly,P21 protein localized on cellular membrane decreased.Both mRNA and protein expression in XIAP were down regulated by mevastatin. All these effects were reversed by mevalonate.Conclusion Mevastatin can inhibite proliferation,induce apoptosis and interfere with cell progression of A549 through mevalonate pathway. Its mechanisms are explained by decreasing the expression of XIAP mRNA and protern.Targeting HMG-CoA reductase,Mevastatin blocks the isoprenylation of P21 protein which affects its anchorage on the cellular membrane.

Objective To investigate the role of P21 protein and survivin on the cell apoptosis of non-small cell lung cancer (NSCLC) induced by mevastatin. Methods The inhibitory effect of mevastatin on A549 and NCI-H520 cell line was evaluated by MTT assay. The cell cycle distribution and apoptosis induction were determined with flow cytometer and transmission electron microscope. The expression of p21 protein was assessed with flow cytometer. The mRNA expression of p21 and survivin was assessed with RT-PCR. Results Flow cytometry showed that mevastatin induced G_0/G_1 cell arrest in NSCLC cell lines. The results indicated that mevastatin caused apoptosis in concentration-dependent manner. Mevastatin produced no change in expression of P21 mRNA and total P21 protein. Concomitantly, P21 protein localized on cellular membrane was decreased. It was also found that mevastatin suppressed the expression of survivin mRNA in NSCLC cell lines. All these effects were reversed by mevalonate. Conclusions Mevastatin inhibited the proliferation of NSCLC cell lines. Mevastatin exerts growth inhibitory effect and induces apoptosis effect by inhibiting mevalonate synthesis. Its mechanisms might involve blockade of the isoprenylation of p21 protein and down-regulation of the expression of survivin mRNA.

Background and purpose:MicroRNAs (miRNAs, miR) are directly involved in cancer initiation, progression and metastasis. Alterations of miRNAs expression in cancer tissue may be reflected in circulation. We attempted to investigate the expression and clinical signiifcance of plasma miR-221 in patients with small cell lung cancer (SCLC). The plasma and tissues levels of miR-221 in 51 SCLC patients and 20 controls were evaluated and compared among various clinicopathological characteristics. Methods: Confirmation of higher miR-221 levels in primary SCLC tissues than normal lung tissues. Evaluation of plasma miR-221 concentrations by comparing results from 51 consecutive SCLC patients and 20 healthy volunteers. Evaluation of the assay for monitoring tumour dynamics in SCLC patients. Results:Expression of miR-221 was signiifcantly higher in SCLC tissues than in para-carcinoma tissues and normal lung tissues. Plasma miR-221 concentrations were signiifcantly higher in SCLC patients than those in normal people (P<0.05). The expression of plasma miR-221 was not associated with gender, and age (P>0.05), correlated with significantly chemosensitivity, overall survival and clinical stage (P<0.05). Cox regression analysis indicated that plasma miR-221 expression and disease stage were found to be significantly independent prognostic factors for the SCLC patients. Conclusion: Plasma miR-221 could be a useful biomarker for cancer detection, monitoring tumour dynamics and predicting malignant outcomes in SCLC patients, and may contribute to clinical decision making in treatments.

Objective This paper will study the effect of the replantation and full crown prosthesis in the treatment of refractory molar periapical disease as well as their related factors .Methods The retrospective analysis will be used in this paper.There were 98 patients who were diagnosed as refractory molar periapical disease in our dental clinic from 2012 to 2015.These 98 cases are divided into two groups:the study group and the control group .This paper will make a comparison of the study group which represents the replantation and full crown prosthesis in the treatment and the control group which re -presents endodontic therapy .Results Through the comparison , the average effective rate of the study group is 93.9%while the control group 65.3%.There is a significant difference between two groups (P<0.01) and the rate of the study group is higher than that of the control group .Conclusion Through the analysis , this paper finds that the joint optimization of replan-tation and full crown prosthesis can provide an unconventional approach to treat refractory periapical of teeth and effectively preserve the tooth .

The therapeutic effects of dermatological preparations for external use are not only dependent on its peculiar pharmacological action of the basis but also closely related to many factors, such as drug concentrations, types of preparation, excipients etc. On the basis of extensively reading the relevant literatures and of author's practical experiences, the above affected faetors were discussed preliminarily.

To monitor the 24 h esophageal pH in patients with gastroesophageal reflux-induced cough (GERC) before and after omeprazole treatment. Methods The prospective study was conducted in Department of Gastroenterology, People's Hospital of Wuhan University from February 2007 to April 2008. Twenty patients with GERC received 40 mg of omeprazole daily and underwent 24 h esophageal pH monitoring before and 8 weeks after omeprazole treatment. Six parameters by Johson and Demeester were adopted for evaluation: pereentage of total time with pH <4, percentage of uptight reflux time with pH <4, percentage of supine reflux time with pH < 4, the number of reflux episodes with pH < 4, the number of reflux episodes longer than 5 min and the percentage of longest reflux duration. Results The values of six parameters before treatment were 13.5±8.5, 12.2±4.0, 15.8 ±4.1, 56 ± 13, 4. 15 ± 1.87 and 26.2 ± 16. 5 respectively; while those after treatment were 2.7± 1.3, 3.9 ± 1.9, 1.9 ± 1.0, 18 ± 11, 1.24 ±0.65 and 7.4 ± 2. 1. There were significant differences in these six parameters between pre-treatment and post-treatment (P<0.01 ). Conclusion The 24 h esophageal pH monitoring shows that omeprazole is effective in treatment of GERC by decreasing esophageal pH.

Objective To study the material basis of hot-natured herb Euodiae Fructus. Methods Euodiae Fructus extract was separated into the chloroform fraction, ethyl acetate fraction, n-butanol fraction and water fraction. With cold syndrome rat model by reserpine, the study confirmed that n-butanol fraction of Euodiae Fructus has influence on energy metabolism in rats. With the modified magnetic polymer microspheres, Euodiae Fructus n-butanol was divided into alkaloids, phenolic acid, fat soluble and water-soluble components, and the component of Euodiae Fructus influencing energy metabolism of model rats was further confirmed.Results The Euodiae Fructus extracts and alkaloids elevated rats temperature, reduced the Na+-K+-ATP activity, increased SDH activity, the content of ATP in liver tissue, the energy charge (EC) value and body heat production, and improve the level of energy metabolism.Conclusion The alkaloid is the material basis of hot-natured herb Euodiae Fructus.

Objective To analyze the pregnancy outcomes of 204 cases of multiple pregnancies following early transvaginal fetal reduction and sum up the experiences of peri-operation nursing care. Methods A total number of 204 twin and triplet pregnancies under treatment in the reproductive medical center from Jan. 2009 to Dec. 2012 were involved in the study. All patients underwent transvaginal multifetal pregnancy reduction during pregnancy of 7 to 8 weeks . The pregnancy outcomes were analyzed , and the nursing measures including preoperative preparation , intraoperative cooperation and postoperative nursing were analyzed . Result The successful rate of multifetal reduction was 100%, and the rate for taking the infants home reached 77.0% ~ 92.3%. Conclusion The outcomes of multifetal reduction for the twin or triplet pregnancy are good . Such nursing measures as perioperative mental care , intraoperative cooperation and postoperative follow-up are key to the alleviation of mental pressure during operation , raise of the success rate of fetal reduction and prevention of postoperative complications.