Objective To get a new approach to conserve the germplasm of Platycodon grandiflorum. Methods A method of vitrification was studied to cryopreserve in vitro shoot-tips of P. grandiflorum, and the regenerated plantlets were observed subsequently. Results Shoot-tips, 2—3 mm length, gotten from in vitro shoots of P. grandiflorum precultured on MS medium supplemented with 5% DMSO and 103 g/L sucrose for 3 d, were loaded with the 60% PVS_2 for 20 min at 20 ℃, and incubated in PVS_2 for 90 min at 0 ℃ prior to a direct plunging into liquid nitrogen (LN) and keeping for 1 d. After rapid thawing in water at 40 ℃, the shoot-tips were rinsed in the MS medium supplemented with 410 g/L sucrose for 20 min, and plated on the filter paper sustained by the MS regeneration medium supplemented with 0.6 mg/L KT, 0.2 mg/L BA, and 0.05 mg/L NAA for 1 d in dark and subcultured on the above regeneration medium for one week in dark prior to exposure to the light. The survival of shoot-tips was up to 80%, and they grew normally. Conclusion The method of vitrification to cryopreserve the germplasm of P. grandiflorum is simple in handling with high in survival and normal in regeneration and can be applied in practice.

Objective To investigate the perceptual priming in brain injured patients. Methods 41brain injured patients, including 24 brain injury and 17 stroke. 99 normal controls, including 39 young, 39 middle aged and 21elderly people. All of them were estimated by the Fragmented Picture Naming Task. Results The patient group had lower priming scores than the young (t=5.558, P<0.001), and middle and elderly-aged (t=3.100, P=0.003) control group in the Fragmented Picture Naming Task. Conclusion Perceptual priming is not only concerned with occipital lobe, but also with frontal and temporal lobes possibly.

This paper presents the principles and the specific circuits of a multifunction generator of ECG signal.It discusses the choice of oarameter of all the circuits included in the small instrument.This instrurrent has been made into prototype and came-into use.

Objective To discuss the role of p38MAPK signal pathway in the process of TWEAK inducing rheumatoid arthritis(RA) fibroblast-like synoviocyte(FLS) to synthesize MMP-9 and look for a new target for RA treatment. Methods RA FLS were primarily cultured and stimulated with TWEAK. Western blot was used to detect the expression of p-p38MAPK and p65 in RA FLS. FLS were pretreated by SB203580 or not. ELISA was used to detect the concentration of MMP-9 in cell-cultured fluid. The mRNA expression of MMP-9 was measured by RT-PCR. Results TWEAK( 100 ng/ml) can make p38MAPK phosphorylated and increased the expression of p65 protein in the cell nucleus. SB203580 can partially inhibit the expression of MMP-9 and MMP-9 mRNA produced by RA FLS which is induced by TWEAK. Conclusion TWEAK induced RA FLS to synthesize MMP-9, in that process, the p38MAPK signal trausduction pathway was in active state, and induced the expression of NF-κB.

Gut microbiota plays an important role in the maintenance of physiological function and genesis of some gut diseases. Brain-gut axis,as an important link between brain and gastrointestinal tract,is a requisite of gut microbiota stability. The dysfunction of brain-gut axis may lead to intestinal dysbiosis through activation of intestinal immune activity. Conversely,intestinal dysbiosis can influence nervous system development and may cause dysfunction of brain-gut axis,in which vagus nerve and metabolites in serum may be the critical factors. This article reviewed the advances in study on interaction between gut microbiota and brain-gut axis.

Objective To summarize the clinical features of brucellosis and investigate the experience of diagnosis and treatment of the disease .Methods Data from epidemic reports and clinical characteristics from 172 ca-ses of patients with brucellosis were retrospectively analyzed .Results 130 out of 172 cases of were diagnosed as bru-cellosisaise.Of the misdiagnosed 42 cases,13 cases were misdiagnosed as lumbar spine ,10 cases as upper respiratory tract infection 8 cases as pancytopenia,5 cases as orchitis epididymitis,2 cases as hepatitis,1 case as prostatitis, 1 case as cervical disease ,1 case as myocarditis ,1 case as rheumatoid arthritis .And after treatment ,142 cases recov-ered completely,16 cases improved.The total efficiency was 100.0%.2 cases without standard treatment recurred ,an interval of 3 to 15 months.Conclusion So the clinical manifestation of brucellosis is lack of specificity that misdiag-nosis rate is high;The manipulation combined with standard antibiotic therapy may have certain effects on early -stage brucellosis .

Objective Establishing a trapezoid micro-plate method to detect ABO blood group in serum.Methods Select 32 blood samples containing A,B,O or AB types randomly.Use orthogonal design principle to select the optimum conditions of the blood plasma dilution,erythrocytes density,temperature and static time.The mix-blood samples are serially diluted.The agglutinating and nonagglutinating gray zones are also defined.Based on the optimum conditions and criterions,the reproducibility test,sensitiveness test,accuracy test,lipaemia interference test and haemolysis interference test are carried out.6 ABO subgroup(AM,AX,A2B,A3B,AXB,BM) and 5 irregular antibody(anti-M,anti-N,anti-P1,anti-Leb,anti-HI) are detected to evaluate the ability of the trapezoid micro-plate method to detect special samples.Results The trapezoid micro-plate method's repetitiveness tests coincidence is 100%.Its sensitivity is identical to the test tube method.The accuracy is 99.99%.In the 47 various kinds of lipaemia blood samples' anti-interference tests,the correct interpretation at one time is 100%.In the haemolysis interference tests,when hemoglobin content are below 20g/L,there are no obvious influences found.When sever haemolysis appears(blood plasma hemoglobin content are above 20g/L),there is no effect on the agglutination,but make a difference to the nonagglutinating.The ability to dectect 6 ABO blood subgroups and 5 irregular antibody blood samples is great.Conclusion The trapezoid micro-plate method not only has good ability to detect the ABO blood group both in the red cell typing and serum typing,but also enhances the accuracy in detecting the agglutinating blood samples and improves the exactitude of the tests.

Objective To evaluate the performance of the remifentanil target-controlled infusion(TCI) system(Minto model) during laparoscopic surgery under inhalation and intravenous anesthesia.Methods A total of 15 patients scheduled for laparoscopic surgery received remifenanil by TCI,and the anesthesia was maintained with inhalation of isoflurane and intermittent intravenous vecuronium.The target remifentanil plasma concentration(Cp) was increased step by step,with the concentration changed at intervals of 30 minutes.The beginning Cp was 3 ng/ml,then increased to 6 ng/ml,9 ng/ml.Blood samples were taken from radial arterial before anesthesia induction and 30 minutes after adjustment of Cp to determine the concentration of remifentanil using high performance liquid chromatography-tandem mass spectrometry(HPLC/MS/MS).The performance of the TCI system was evaluated by median performance error(MDPE),median absolute performance error(MDAPE) and wobble.Results The MDPE,MDAPE and wobble of the remifentanil TCI system were 8.78%,16.11% and 14.55%,respectively.The measured concentrations of remifentanil were positively correlated with Cp(r=0.891,P=0.000).The linear equation was Y∧ =1.1046X+0.1837.Conclusions The remifentanil TCI system with Minto pharmacokinetic model can meet the requirements of clinical anestehesia for Chinese patients within clinical treating concentration.

0.05), while the values of BIS at all the time points in group A were significantly higher than those in groups B and C; meanwhile the BIS at different time points in group B were significantly higher than those in group C (both P

Objective To analyze the results of distribution of mycoplasma pneumoniae IgM antibody and DNA test,and ex-plore its application in diagnosis of Mycoplasma pneumoniae infection in children.Methods Retrospectively analysed myco-plasma pneumoniae antibody IgM (MP-IgM)and mycoplasma pneumoniae DNA (MP-DNA)results,114 374 passengers and 8 014 passengers,in 0 ~ 16 year-old children of Shengjing Hospital Affiliated to China Medical University from year 2013 to 2014.Analyzed of the relationship between gender,age,season and the positive rate of the MP-IgM and MP-DNA. Collected 5 106 patients with MP-IgM and MP-DNA results simultaneously and analyze the consistency between two posi-tive indexes in different ages.Results ①MP-IgM and MP-DNA positive rate varied in sex,age and season,the positive rate in female was higher than male.The positive rates of MP-IgM and MP-DNA at the age of 1~3 and 7~ 12 years old were higher than other age groups.The positive rate of MP-IgM in spring and autumn was higher,while MP-DNA was higher in summer and autumn and all differences were statistically significant (P < 0.001).②The positive rate of MP-DNA differed in specimens,the bronchoalveolar lavage fluid had the highest MP-DNA positive rate,49.95%.③The positive consistency of MP-IgM and MP-DNA was associated with age,with 4~6 and 7~12 year-olds the highest,respectively 35.2% and 45.8%. Conclusion ①Incidence of children pneumonia mycoplasma infection was mainly in preschool and school-aged children,more female than male,and autumn highest.② The detection of MP-DNA in bronchoalveolar lavage fluid was the best sample type.③For <1 and 1~3 year-olds,combined detection of MP-DNA and MP-IgM can improve diagnostic rates.