Objective:To construct an expression plasmid of siRNA against gene hIAP-2 and to assess its effect on breast cancer cell line MCF-7.Methods: A hIAP-2 siRNA plasmid was constructed with mU6pro vector containing U6 promotor and its RNAi effect on MCF-7 cells was detected by RT-PCR and Western blot.The effects of the plasmid on MCF-7 proliferation were analyzed by MTT assay,on the cell cycle by flow cytometry,and on the cell morphology by Hoechst staining.The activity of caspase-3 was determined with its substrate Ac-DEVD-pNA and the expression of some related proteins was detected by Western blot.Results: The hIAP-2 siRNA plasmid knocked down hIAP-2 expression in MCF-7 cells specifically and obviously,inhibited tumor cell proliferation and arrested the MCF-7 cells at G_(1) phase.After hIAP-2 gene was silenced,MCF-7 cells became multinuclear and had macronucleus;procaspase-3 was activated and its expression was increased.The protein levels of I?B?(p37) and p21~(waf1) increased,of NF-?B(p65) decreased and Cyt C remained unchanged.Conclusion: hIAP-2 in MCF-7 cells plays a crucial role in cell proliferation and cell cycle progression.Mitotic cell death might be the main way of the cell death;G_(1) arrest after DNA damage might be associated with the p21~(waf1) increase.

Objective To investigate the effects of different times of needle retention on cognitive function in patients with vascular cognitive impairment no dementia (VCIND). Methods One hundred and four VCIND patients were randomly allocated to group A of 35 cases, group B of 34 cases and group C of 35 cases. All the three groups received acupuncture plus medication. Of them, group A was given scalp acupuncture with 30 min retention of needles;group B, with 1 hr retention of needles;group C, with 10 hrs retention of needles. The Montreal Cognitive Assessment (MoCA) score and the ADL score were recorded in the three groups of patients before and after treatment. Results There were statistically significant pre-/post-treatment differences in the MoCA score and the ADL score in every group (P<0.05). There were statistically significant post-treatment differences in the MoCA score and the ADL score between group A or B and group C (P<0.05). Conclusions Acupuncture is an effective way to treat VCIND. It can improve cognitive function in the patients. Scalp acupuncture with 10 hrs retention of needles has a more marked effect.

With the development of global warming,urbanization,ageing of the population,it is paid more attention to the human health impacts on the frequent occurrence of heat wave and urban heat island effect.The study methods and advances in impacts of heat wave on human health were reviewed in the present paper.

In recent years,high-temperature heat wave caused an increase in the number of deaths than other climate disasters,the study of the effects of heat waves on the health of urban residents health becomes a subject of concern.Sustained high-temperature heat waves will inevitably cause harm to human health,to understand the characteristics of heat waves is conducive to the prediction of high temperature heat waves and to the establishment of reasonable response measures.This paper mainly introduces the present status of heat wave occur,the impact of heat wave on human health,the influencing factors for excess mortality during heat waves as well as the research methods for heat wave which are commonly used to predict.

Objective To study and compare the metabolism of Chrysanthemum morifolium extracts(CME) in vitro intestinal flora from human,rat,Beagle dog,and rabbit.Methods Intestinal flora of human,rat,Beagle dog,and rabbit and CME were anaerobically cultured at 37 ℃ in vitro. After being extracted by acetic ether,the main metabolites of CME were separated and quantified by HPLC.Results In vitro,CME was easily metabolized by the intestinal flora and the main metabolites in incubation medium were determined in high concentration after 0.5 h.By LC-MS,luteolin and apigenin were identified,respectively;both metabolites were degraded with prolongation of incubation time and the concentrations of luteolin and apigenin were low after 24 h;moreover,CME was quickly metabolized by human,rat,Beagle dog intestinal flora,and gently by rabbit intesinal flora.Conclusion In vitro,CME is easily metabolized by intestinal flora human,rat,Beagle dog,and rabbit and the main metabolites are luteolin and apigenin.

Objective The study analyzes the inadequacies of the research integrity educational practice for the medical postgraduate students in the present stage of China.Furthermore,the paper puts forward improvement suggestions and clears the direction for further efforts.Methods From the perspective of the educational law about cognition,emotion,will and behavior,the comparative analysis method is used,based on the rich experience in the United States.Results There are inadequacies of the research integrity educational practice for the medical postgraduate students in China,including that students lack knowledge learning motivation,and the pattern of education is simple.There are not enough emotional experience channels and the participation of students.Students lack honesty will training,and the intensity of supervision and punishment is not strong.The honesty recognition mechanism is weak,and cannot be used to guide practice.Conclusions In order to improve the level of the research integrity for the medical postgraduate students in China,we should optimize the system,follow the law of education,strengthen the transmission of knowledge,put the academic honesty education into normal work,enhance the emotional experience,build a participatory mechanism,highlight the positive guide,and increase the intensity of punishment.

OBJECTIVE:To put forward suggestions for the legal regulation on the financial conflict of interest of researchers in drug clinical trials.METHODS:The definition and harm of the financial conflict of interest of the researchers in clinical trials were analyzed,and the legal measures of FDA on regulating and controlling the financial conflict of interest of the researchers were evaluated.RESULTS & CONCLUSIONS:The Chinese legislature should draw useful legal experiences from USA to establish suitable legal measures to regulate and control the financial conflict of interest of researchers in clinical trials,clarify the definition of the financial interest of researchers,require the applicant for clinical trial to disclose his financial interest and the applicants for drug registration to submit the statement about the financial conflict of interest of researchers to the drug administration department.

Objective To investigate the effects on intraocular pressure and blood pressure following anterior chamber paracentesis in acute primary angle closure glaucoma with high blood pressure.Methods 64 eyes of 64 glaucoma patients with high blood pressure were received anterior chamber paracentesis.the intraocular pressure and blood pressure were measured before and after paracentesis.Results The mean systolic blood pressure(SBP) and diastolic blood pressure(DBP) before paracentesis of 64 glaucoma were 195.10?15.24mmHg and 117.1?11.76mmHg respectively,after administration of paracentesis,the SBP and DBP were 138.82?20.86mmHg and 93.19?12.07mmHg respectively,the intraocular pressure also significantly decreased from 68.64?10.05mmHg to 10.66?4.41mmHg.There was statistical difference in SBP,DBP and intraocular pressure before and after administration of paracentesis.Conclusions Administration of paracentesis in acute primary angle closure glaucoma with high blood pressure can significantly reduce intraocular pressure and blood pressure simultaneously.

[Objective]To evaluate the effects of mosapride combined with acupressure at selected time and psychotherapy on constipation-predominant of irritable bowel syndrome.[Methods]A total of 86 patients with constipation-predominant of irritable bowel syndrome(C-BS) randomly divided into two groups:the treatment group(n=43) and the control group(n=43). Every patient in the study was diagnosed C-BS according to Rome II criteria. Patients in control group took mosapride (5mg,tid) and in treatment group acupressure at selected time and psychotherapy were givn besides mosapride.[Results]The total effective rate of the treatment group was 86.0%,which was superior to that of the control group(60.5%)(P

The prevalence rate of diabetes is continuously increasing in China, and diabetes characterizes as high morbidity, low diagnostic rate, low cure rate, and low good control rate, which consequently resultes in the occurrence and progression of various diabetic complications and dramatically decreases the quality of life as well as the survival of diabetic patients.So it is crucial for clinician to screen diabetes early, make the diagnosis early, and start the treatment promptly.This article systemically summarizes the clinical manifestations, diagnostic criteria, typing, pathophysiology, complications, and management of diabetes.