Objective To evaluate the clinical value of cervical conization in diagnosis and management of cervical carcinoma in situ.Methods The clinic-pathological data of the 65 patients with cervical carcinoma in situ from Jan 2004 to Feb 2007 were analyzed retrospectively.Results(1)Of the 65 patients,59 cases had received the colposcopical multiple biopsies before the cervical conization and there was a correlation in pathology between cervical conization and colposcopical multiple biopsies in 46 cases(78.0%),but there was not much correspondence between cervical conization and colposcopical multiple biopsy in 13 cases(22.0%).(2)30 patients were received hysterectomy or cervical conization again.Patients with positive margins were significantiy more likely to have residual disease than those with negative margins(P

Objective To evaluate the predictive value of cord blood bilirubin levels for subsequent neonatal hyperbilirubinemia in term infants with ABO hemolytic disease.Methods A total of 292 term newborns with ABO hemolytic disease admitted from August 1,2011 to July 31,2012 were enrolled.Cord blood bilirubin levels were analyzed and the clinical characteristics of the neonatal hyperbilirubinemia group (n=34) and non-hyperbilirubinemia group (n=258) were compared.A receiver operating characteristic (ROC) curve analysis was performed to identify the predictive value of the occurrence and cut-off point of hyperbilirubinemia in term infants with ABO hemolytic disease.Paired-t-test,Chi-square test and Spearman correlation were used for statistical analysis.Results Of the 292 term infants with ABO hemolytic disease,34 cases had hyperbilirubinemia,with an incidence of 11.6％.Cord blood bilirubin levels were significantly associated with the presence of hyperbilirubinemia.The mean cord blood bilirubin level in infants who developed hyperbilirubinemia was (52.4± 13.2) μ mol/L,and was (35.0±8.0) μ mol/L for those who did not develop hyperbilirubinemia (t=7.540,P=-0.001).When cord blood bilirubin concentration increased,the incidence of hyperbilirubinemia gradually increased (x2=113.715,P＜0.001; rs=7.19,P＜0.001).The ROC area under the curve of 0.882 (standard error 0.005,95％CI:0.873-0.891,P＜0.001) was significant in predicting neonatal hyperbilirubinemia by cord blood bilirubin,and the occurrence of hyperbilirubinemia increased with increasing cord blood bilirubin level.Neonatal cord blood total bilirubin ≥ 50 μ mol/L predicted hyperbilirubinemia,and the positive predictive value was 0.683,negative predictive value was 0.959,sensitivity was 0.690 and specificity was 0.958.Conclusions Cord blood bilirubin level is useful in predicting subsequent neonatal hyperbilirubinemia in term infants with ABO hemolytic disease.

OBJECTIVE:To explore the observation and nursing points of hemorrhage in intravenous thrombolysis after using alteplase. METHODS:128 patients underwent alteplase intravenous thrombolysis were selected as the research object. The occur-rence of hemorrhage in each part were observed within 48 hours after medication and nursed timely. RESULTS:128 patients re-ceived intravenous thrombolysis. 48 h later,26 patients suffered from hemorrhage complications (20.3%). Among them,there were 6 cases of nose bleeding (4.7%),8 cases of subcutaneous congestion (6.3%),6 cases of gingival bleeding (4.7%) and 6 cases of gastrointestinal bleeding(4.7%). There was no significant difference in the incidence of complications among those groups (P>0.05). The time of nose bleeding was (3.2 ± 1.3) h,the time of subcutaneous congestion (3.5 ± 1.2)h,the time of gingival bleeding(1.5±0.7)h,and the time of gastrointestinal bleeding(4.1±1.8)h. The time of gingival bleeding was earlier than that of other groups,with statistically significant difference(P<0.05). There was no statistically significant difference among other groups (P>0.05). CONCLUSIONS:In alteplase intravenous thrombolysis,nurses should closely monitor hemorrhage and complications, especially the complication of gingival bleeding. Find out the complications timely and nurse it as early as possible.

According to the anatomic classification,refractive surgery is divided into keratorefractive surgery,phakic refractive surgery and scleral refractive surgery.Most procedures for the correction of ametropia are performed on the cornea,with laser-assisted in situ keratomileusis(LASIK) being the most common one.LASIK has shown its limitations,however.For the correction of higher myopic and hyperopic refractive error,the procedure has considerable undesired effects,such as loss of contrast,glare,and iatrogenic keratectasia.Therefore,a variety of intraocular lens,known as phakic refractive surgery,have been designed to achieve full correction of high myopia and hyperopia without loss of accommodation.Phakic refractive surgery is different from kerato-or scleral refractive surgery in that it involves phakic intraocular lens,non-phakic intraocular lens and clear lens extraction +introcular lens.The author will outline recent progress in phakic intraocular lens and provide unifying concepts that are accepted by ophthalmologists.

Objective: To investigate whether hypoxia can affect the expression and secretion of connective tissue growth factor(CTGF) and fibronectin(FN) in primary cultured rat renal cortical myofibroblasts . Methods: The primary cultured rat renal cortical myofibroblasts were subjected to hypoxic (1%O_2) or normoxic (21% O_2) conditions for a variety of times. The protein levels of HIF - 1?, CTGF and FN protein were analyzed by Western blotting in both the whole cell lysates and supernatant culture medium 6 h,12 h and 24 h after incubation, respectively. RT-PCR was carried out to measure the levels of FN mRNA at different time points (2 h,3 h,6 h and 12 h). The activity of gelatinase MMP-2 and MMP-9 in the supernatant from the cultured cell medium was assayed by gelatin zymography. Results: The expression of HIF - 1?was induced at h6 in cells under hypoxia incubation. The levels of cellular CTGF protein were increased in hypoxia treated myofibroblasts at h6 (175%?52%),significantly elevated at h12 (347%?67%,P

Objective To study the prognosis value of the expression of vascular endothelial growth factor and it's acceptor(VEGF,Flk-1) and microvessel density(MVD) gene proteins in the breast carcinoma and paracarcinoma tissue.Methods Immunohistochemical technique was adopted to detect the protein expression of VEGF,Flk-1 and MVD in 76 cases of breast carcinoma and 10 cases paracarcinoma tissue.Results (1)The positive expression of VEGF,Flk-1 were 59.3% and 61.3% in the carcinoma group,which were higher than in the paracarcinoma tissue(P

Objective To explore the best anticoagulant ratio in SD rats .To analyse the influence of insufficient blood specimens volume on coagulation tests .Methods 60 rats were divided into 2 groups.According to the method of vacuum blood, collect abdominal aortic blood after fasting 12 hours.The first group 20 rats were used only for routine blood test.Fully automatic hematology analyzer detected hematocrit and platelet .The second group 40 rats were used for coagulation test .Every rat was collected 2 blood specimens with different anticoagulant ratio [ the proportion of sodium citrate anticoagulation and the whole blood (vlume∶volume)]1∶9(the control group)and 1∶5( the experimental group),1∶8( the experimental group) and 1∶7( the experimental group).Get plasma without platelet through centrifugation.Fully automatic blood coagulation analyzer detected prothrombin time , activated partial thromboplatin time , thrombin time and Fibrinogen.Results HCT (%) and PLT (x 109/L) in SD rats were respectively 41.7 ±2.9 and 1114 ±173.As anticoagulant ratio was increased ,PT, APTT and TT were extended and FIB was decreased .Compared with the control group ,these PT,APTT,TT,FIB four results of 1∶8 group were not statistically different , of 1∶5 group were statistically different, these PT,APTT,FIB three results of 1∶7 group were not statistically different , TT of 1∶7 group was statistically different.Conclusions The detection of coagulation project coagulation test results were affected by the proportion of anticoagulant and blood .1∶9 was the best anticoagulant ratio in SD rats , 1∶8 can also.Rat had its unique physiological characteristics .The results could provide reference for the evaluation of rats .

Objective The present study was initiated to determine the expression profiles of the Fox genes in normal Balb/c mouse liver and their dynamic expression changes during fibrogenesis induced by experimental bile duct ligation(BDL).Methods RT-PCR was employed to detect 18 Fox family members including Foxo1,Foxo3,Foxm1,and Foxl1 in normal mouse liver.After mice were bile-duct-ligated,real time fluorescence quantitative PCR(FQ-PCR)was performed to ananlyze the dynamic mRNA expression changes of 9 inflammation-or proliferation-related Fox family genes.Results All the 18 Fox genes were found to be exDressed in the normal mouse liver and bile duct ligation profoundly influenced the expression of Fox transcriptional factor family genes.The expression of Foxol was significantly reduced by BDL,while FoxoL1 and Foxom1 expression were enhanced in this roodel.Moreover,the expression of Foxo1 and Foxo3 were the highest among the Fox members.Conclusion The Fox family genes-related to inflammation and proliferation were dynamically changed during BDL-induced liver injury,it indicates these genes were involved in the pathogenesis of liver fibrosis.

BACKGROUND: Resin adhesives include total-etching, self-etching and one-step adhesive. The studies concerning their adhesive properties remain different.OBJECTIVE: To compare the bonding strengths of fiber posts cemented to root canals with etch-and-rinse adhesives and self-etch adhesives, and to observe the physical interface bonding conformation using laser scanning confocal microscope so as to understand the retention of macro-and micro-morphology relationship.DESIGN, TIME AND SETTING: In vitro comparative observation was performed at the laboratories of Prosthodontics and Oral Surgery, Ninth People's Hospital, Shanghai Jiao Tong University from March 2008 to March 2009.MATERIALS: MATCHPOST fiber posts (Bisco, USA); etch-and-rinse adhesives (One-step, Bisco, USA) and self-etch adhesives (Panavia F adhesive system, Japan), fluorescein (Sigma, USA).METHODS: A total of 10 upper central incisor teeth, which had been freshly extracted for periodontal reasons, were selected,and the crown, 1 mm from the enamel-cementum junction, was cut. They were randomly divided into 2 groups with 5 specimens in each group. The fiber posts were bonded to root canals with One-Step or Panavia F which was tagged with rhodamine B.MAIN OUTCOME MEASURES: Each specimen was sectioned into two 2 mm-thick slices. A push-out test was made in a universal testing machine (EZ20), and confocal laser scanning microscopic (CLSM) analysis was performed to evaluate the primer penetration into dentin. The thickness of adhesive layer was measured by random software.RESULTS: The bonding strength of the group cemented with One-step was significantly greater than Panavia F (P < 0.05). The bonding strength of root crown was significantly greater than root apex (P < 0.05). One-step represented in CLSM image showed numerous and longer resin tags than Panavia F (P < 0.05).CONCLUSION: Etch-and-rinse adhesives (One-step) gained higher bonding strengths than self-etch adhesives (Panavia F).Resin binder to bond strength of fiber post and neck was greater than in the root apex.

Objective To evaluate the clinical effects of low-dose glucocorticoid hormone adjuvant treatment for mycoplasma pneumonia.Methods 118 cases diagnosed with mycoplasma pneumonia were randomly divided into two groups,including conventional treatment group (61 cases) and hormone treatment group (57 cases).The treatment group was given to the small dosage hormone,the control group was hospitalized for the same time which had not used the hormone the trouble.Both of two groups were given relieve a cough,breathe heavily evenly,the atomization inspiration and so on the complex therapy,and application large ring lactone class medicine including erythromycin or arch mildew element anti-infection treatment.On the basis of conventional treatment,the hormone treatment group was added with intravenous infusion of dexamethasone or oral prednisone.Compared with two groups of curative effects indexes including the defervescence time,the cough remission time,the moist crackles disappearance time and the hospitalization time.Results The defervescence time,the cough remission time,the moist crackles disappearance time and the hospitalization time were significantly lower in the hormone treatment group than those of the control group (t =-6.36,-2.04,-7.91,all P ＜ 0.05).Conclusion Low-dose glucocorticoid hormone adjuvant treatment could have the better curative effects for mycoplasma pneumonia for children.