Objective:To investigate the therapeutic effect of sequential blood purification on rescuing acute grave intoxication.Methods:Twenty eight patients with acute intoxication were treated by HP+CVVH during 2002 and compared them with thirty patients without hemopurgation from 1998 to 2002.Results:The group of hemopurgation was better than non-hemopurgation group in the time of revive,the rate of recovery and death,and the total dose of atropine.Conclusion:Hemopurgation has decisively effect on rescuing all kinds of intoxication with toxins and drugs.It could quickly ameliorate symptom,raise success rate and reduce mortality;Hp combining with CVVH are suitable for the patients with toxicsis who had cardiac and respiratory failure,brain edema,and dielectric disorder.

Objective:To investigate the effect of vitamin E supplementation on oxidative stress in patients with MHD(maintenance hemodialysis).Methods:58 patients with MHD and 20 healthy volunteers were involved in this study.The patients were administered 400mg/d of vitamin E for 6 weeks.The plasma levels of oxidative parameters including vitamin E(VitE),malondialdehyde(MDA),advanced oxidation protein product(AOPP),superoxide dismutase(SOD),activity of glutathione peroxidase(GSH-PX) were measured at the initial and the end of VitE supplementation respectively.Results:Plasma levels of AOPP and MDA(187.27?54.9?mol/L vs99.05?26.2?mol/L,7.74?2.17nmol/ml vs3.06?1.70nmol/ml)were significantly higher in patients with MHD compared with healthy controls(P

OBJECTIVE To improve the competition ability of the hospital,emphasis of the importance of hospital infection management is necessary and urgent.METHODS Our experience on improving culture level in hospital infection management was introduced.RESULTS We improved management efficiency by fluent information,altered the concept of value by bettered the management means,and brought the renovation idea by lifting the qualification of management staff.CONCLUSIONS Correctly making the most use of the unique principles of culture in hospital infection management could be benefit for creating a good atmosphere,establishing nice profession image,and setting up norm criterion,which could facilitate the controlling hospital infection.

The prevalence rate of diabetes is continuously increasing in China, and diabetes characterizes as high morbidity, low diagnostic rate, low cure rate, and low good control rate, which consequently resultes in the occurrence and progression of various diabetic complications and dramatically decreases the quality of life as well as the survival of diabetic patients.So it is crucial for clinician to screen diabetes early, make the diagnosis early, and start the treatment promptly.This article systemically summarizes the clinical manifestations, diagnostic criteria, typing, pathophysiology, complications, and management of diabetes.

Pediatric Critical Care Medicine have developed rapidly and played a big role in the clinical treatment in China in recent years.But we axe now still in lack of unified documents as a guidline because of our late development and difference between regions.We should pay more efforts to formulate and perfect the standardization constructions of pediatric intensive care unit,with the help of The Construction and Management of Critical Care Medicine.In this article,we stated the present situation of development,operational mode,personnel and equipment configuration,monitoring and treatment technology,process specification and quality index in pediatric intensive care unit.And we also offerd some proposal about that.

The progress and development of extracorporeal life support (ECLS) is suprising and on-going,especially extracorporeal membrane oxygenation (ECMO) has become an accepted therapeutic modality for patients who have failed conventional therapy with respiratory and/or cardiac support.Blood purification developed from hemodialysis is designed to remove substances from the circulation and now includes hemodialysis,hemofiltration,immunoadsorption,continous blood purification and peritoneal dialysis.Plasma exchange and hemoperfusion play an important role in critically system diseases,i.e.,some kidney diseases,neurologic diseases,liver failure,metabolic diseases,connective tissue disease,infection diseases,intoxication and so on.

Objective To investigate the oxidative stress,microinflammation and their relationship in patients with MHD(maintenance hemodialysis)and to evaluate the effect and safety of vitamin E supplementation in patients with MHD.Methods Fifty-eight patients with MHD and 20 healthy volunteers were involved in this study.The patients were administered 400 mg/d of vitamin E for 6 weeks.The levels of oxidative vitamin E(Vit E),malondialdehyde(MDA),advanced oxidation protein product(AOPP),superoxide dismutase(SOD),activity of glutathione peroxidase(GSH-PX),microinflammation cytokines C-reactive protein(CRP),interleukin-6(IL-6),interleukin-10(IL-10) and tumor necrosis factor(TNF-?)in plasma were measured at the beginning and the end of administering Vit E.Results 400 mg/d of Vit E supplementation decreased the levels of plasma AOPP,MDA,CRP,TNF-?(P

Objective To investigate Hepatitis B Virus (HBV) infection status in placentas of Hepatitis B Surface Antigen (HBsAg) positive pregnant women during middle and late period of pregnancy. Methods One hundred and sixty seven placentas from HBsAg positive pregnant women were collected, including 158 term placentas and 9 aborted second trimester placentas. HBsAg and hepatitis B core antibody (HBcAg) in placental cytotypes were detected by immunohistochemistry. Results Of the 158 term placentas, HBV infection rates in decidual cells (DC), trophoblastic cells (TC), villous mesenchymal cells (VMC) and villous capillary endothelial cells (VCEC) were 66 46% (105/158), 58 23% (92/158), 27 22% (43/158) and 12 66% (20/158), respectively. The HBV infection rates gradually decreased from maternal side to fetal side of placentas. Both HBsAg and HBcAg were mainly located in the cytoplasm of infected cells. The positive cells were mostly in focal distribution. One out of 9 aborted second trimester placentas was infected. Conclusions HBV infection was found in different cells of term placentas. The positive rates for HBsAg and HBcAg in placentas were gradually decreased from maternal side to fetal side. The positive rate was low in aborted second trimester placentas.

Objective To evaluate the microRNA role in the pathogenesis of alcohol dependence.Methods By computer retrieval Medline,mirbase,PubMed,Chinese journal full-text database (CNKI),ten thousand database and VIP database,manual retrieval relevant references.Literature retrieval time limit are built from the library until July 2014.English database to Alcoholism,Alcohol Dependence,Alcohol Abstinence,Alcohol Amnestic Disorder Fetal Alcohol Spectrum Disorders,mirna as keywords retrieval;Chinese database to Alcoholism,Alcohol Dependence,Alcohol Abstinence,Alcohol Amnestic Disorder Fetal Alcohol Spectrum Disorders,microRNA,etc.Retrieved from the database to the related literature in July 2014.Results A total of 26 studies were recruited including 8 Chinese literatures and 18 English literatures.Conclusion Abnormal expression of miRNAs will become the next research direction.MicroRNAs may become new biomarker of alcohol dependence on diagnosis,may also makes the molecular drug targets,or the molecular simulation for new drug research and development,this could be for the treatment of alcohol dependence provides a new means.