Objective:To revise the children's perception of interparental conflict scale (CPIC) developed by Grych and his colleagues and examine its reliability and validity Method:Data were collected from a sample of 303 primary school students tested with the CPIC scale Results:The results of factor analysis and hierarchical cluster analysis showed that the revised CPIC had a good construct validity; and ?coefficient of the whole scale is 0 879, the reliability coefficient of split-half is 0 865, and the ?coefficient of each dimension was more than 0 657 Conclusion:The CPIC scale is suitable for Chinese children after revision

Objective To clone different lengths cell division autoantigen1(CDA1) promoters(CDA1P) and measure their activities in order to provide basis for fumctional study of CDA1P.Methods Different lengths CDA1P were amplified by PCR using mouse liver genomic as template and cloned into BglⅡ digested pGL3-basic plasmid to produce recombinant plasmid pGL3-basicmCDA1P,then they were transfected into Lewis lung cell line(LLC) and RAW 264.7 cells differently,these cells were collected after transfected for 48 h,finally luciferase activities of pGL3-basic-mCDA1P were measured.Results ①Different lengths CDA1P were cloned and characterized by restriction enzymes.②Different lengths CDA1P were ligated into pGL3basic vector,and were proved with PCR and DNA sequencing .③Activities of pGL3-basic-mCDA1A were different in the two kinds of Leuwis and mononuclear macrophage cells(RAW264.7 cells).Conclusion The different activities of CDA1P may be related with different cellular types.

Objective: Exploring the reliability and validity of Chinese version of The Social Support Appraisal (SS-A) scale developed by Vaux et al (1986) and the characteristics of adolescent's social support.Methods:Using the scale, 515 junior and senior high school students were investigated.Results:(1) The SS-A scale had internal consistency of 0.91 for the scale and of 0.81-0.84 for three dimensions and a three-factor model was supported by the data (?2/df

Objective Through the investigation of the iodine content in residents drinking water in Jianjiang River Basin of Guangdong,the scope and distribution of waterborne high iodine areas in this region were grasped so as to provide a basis for taking appropriate prevention and control measures.Methods All towns (streets) covering seven counties (cities,districts) of Jianjiang River Basin were selected by cross sectional screening method in 2012 and 2013,which were divided into mainly-investigated town (street) and generally-investigated town (street) according to the direction,geographical position and other situations of all branches of Jianjiang River.In each mainly-investigated town (street),two natural villages were selected according to the river direction and terrain conditions,and one water sample was collected from each village.In each generally-investigated town (street),one administrative village was selected respectively according to the locations (east,west,south,north and middle);two natural villages were selected from each administrative village,and one water sample was collected from each natural village.The water supply types of each natural village were investigated,including centralized water supply,decentralized water supply and river (reservoir) water.If the iodine content of the water samples was higher than 100 μg/L in the natural villages,then 10％ more water samples were collected.The inductive coupling plasma mass spectroscopy (ICP-MS) was adopted for water iodine detection.Results A total of 3 583 residents drinking water samples from Jianjiang River Basin were investigated,and the median of water iodine was 15.1 μg/L;the median of water iodine in reservoirs,river,mountain spring,well water was 12.1,9.8,2.5 and 18.6 μg/L,respectively.Iodine content of 341 water samples was ＞ 100 μg/L,accounting for 9.5％;228 water samples were ＞ 150 μg/L,accounting for 6.4％;58 water samples were more than 300 μg/L,accounting for 1.6％.The water samples with iodine content higher than 100 μg/L were all from decentralized well water.There were 18 natural villages which could be determined as the waterborne high iodine areas (water iodine ＞ 150 μg/L);they were distributed in 16 administrative villages in 14 towns (streets) of 5 counties (cities,districts) and had a registered population of 23 915 in total.The difference of water iodine between the 5 counties (cities,districts) in middle and lower reaches of Jianjiang River Basin (24.7,28.6,20.5,23.2,24.0 μg/L) and the 2 counties (cities,districts) in upper reaches (12.0,10.9 μg/L) was statistically significant (Z =13.5,P ＜ 0.05);the difference of water iodine between coastal and non-coastal areas (12.5 vs.5.9 μg/L) was statistically significant (Z =6.1,P ＜ 0.05).Conclusions The iodine content in surface water of Jianjiang River Basin is generally lower;,the high water iodine phenomenon dispersedly occurs in the underground water (well water),and the iodine content is different in different areas;the iodine content of the middle and lower reaches is higher than that of the upper reach,and the iodine content of the coastal alluvial areas is higher than that of the non-coastal areas.The natural villages with waterborne high iodine are spottily distributed in the coastal areas of all backbones and branches of Jianjiang River,but not connected together basically.

Objective To understand the situation of iodine of Taishan nuclear power station employees,and assess the relationships between iodine nutrition and thyroid nodule of Taishan nuclear power station in Taishan,Guangdong.Methods Totally 300 people were selected from of Taishan nuclear power station through stratifiedrandom sampling method,random urine samples were collected and urinary iodine level was tested in 2014.According to 20 to 29 years old,30 to 39 years old,40 to 59 years old,3 age groups were sampled,and each age group was 100 people.Drinking water and edible salt samples of the of nuclear power station were collected and iodine level was tested.Daily dietary intake of the staff was investigated,and daily iodine intake was calculated.Result of thyroid B ultrasonic of the employees (1 091) were studied,and detection rate of thyroid nodule was calculated.Results Totally 308 urine samples were tested,and the median urinary iodine was 193 μg/L.The difference of urinary iodine between 20-year-old group (201 μg/L) and 30-year-old group (165 μg/L) was statistically significant (P ＜ 0.05).A total of 9 iodized salt samples were collected,with an average iodine value of (24.4 ± 0.5)mg/kg.A total of 9 water samples were collected,the median of water iodine was 20.5 μg/L in 3 centralized water supply samples.The daily iodine intake per capita of the staff was 274.0 μg/d,the highest contribution among all the varieties of food was iodized salt,the second was seafood,which were 45.26 percent and 41.86 percent,respectively.A total of 99 thyroid nodules and suspicious nodules were detected in 1 091,and the prevalence was 9.07 percent (99/1 091).The prevalence of people aged 40-59 [15.6％ (22/141)] was significantly higher than those of people aged 20 to 29 [8.1％ (51/633)] and aged 30 to 39 [8.2％ (26/317)],and the differences were statistically significant (P ＜ 0.05).Conclusions The iodine nutrition of Taishan nuclear power station employees is appropriate as a whole.The detection rate of thyroid nodules is higher in people aged 40 to 59.

Objective To find the relevant factors of the young children’ physical and intellectual development in Guangdong province for children growth promotion.Method Physical status of 3 844 children 0-18 month and intellectual status of 749 children from urban,suburb and rural area in Guangdong province and their relevant factors were investigated.Then multiple regression analysis was applied statistically for their physical and intellectual development.Results Adjusted the interfering factors,the young children’ physical development was significantly correlated to their birth place,monthly income of family,monthly expense of food,paternal education degree,duration of breastfeeding,duration of exclusively breastfeeding,time of introduction of complementary food and intake of egg and vegetable.And their intellectual development was significantly correlated to their birth place,maternal education degree,time of introduction of complementary food and intake of formula,meat,bean and egg.Conclusion Increase of socioeconomic level,popularization of nutrition knowledge,promotion of breastfeeding and timely and reasonable introduction of complementary food are main effective measures to improve children’s development.

Through the investigation of the 35 training institutions that recruit Master of Public Health (MPH) and the analysis of MPH candidates and admissions, training objectives, curriculum, practices, and dissertations and other research contents, we understood the current status of MPH training, and found problems in the training process. To improve the quality of training of full-time masters of public health, we should step up publicity efforts, adjust enrollment structure, expand enrollment, make clear the raining objectives, improve curriculum, practice session, and reform the paper evaluation system, etc.

AIM:To investigate whether Chinese yellow wine has influences on homocysteine ( Hcy )-induced dysfunction in rat endothelial progenitor cells (EPCs).METHODS:Rat bone marrow was extracted to harvest mononucle-ar cells ( MNCs) by density gradient centrifugation .The MNCs were plated on fibronectin-coated culture dishes , and were induced into EPCs by EGM-2 complete medium supplemented with cell growth factor .The adherent cells were collected 7 d later for all studies .EPCs were characterized as adherent cells double positive for DiI-ac-LDL uptaking and lectin binding by direct fluorescent staining under a laser scanning confocal microscope .The viability, migration, apoptosis and in vitro vasculogenic activity of the EPCs were determined by MTT assay , Transwell chamber assay , apoptosis kit and in vitro vas-culogenesis kit, respectively.RESULTS:Compared with control group, the viability, migration and in vitro vasculogenic capacity of the EPCs in Hcy group were significantly decreased (P<0.01).Compared with Hcy group, yellow wine group and red wine group both significantly improved the viability , migration and in vitro vasculogenic capacity of Hcy-induced EPCs (P<0.01).Compared with control group, yellow wine group and red wine group both significantly improved the a-bove-mentioned functions of EPCs (P<0.05).However, no significant difference of apoptosis in all groups was observed . CONCLUSION:Hcy may result in dysfuction of EPCs .Treatment with yellow wine improves Hcy-induced EPC functions .

OBJECTIVETo analyze the clinical characteristics of protracted bacterial bronchitis (PBB) in children.

METHODThe clinical data of patients seen from October, 2010 to March, 2014 in Department of Respiratory Diseases of our hospital were retrospectively analyzed. Inclusion criteria were over 4 weeks cough, receiving fiberoptic bronchoscopy, positive bacterial culture and (or) the increased percentage of neutral granulocytes in bronchoalveolar lavage fluid (BALF).

RESULTTwenty eight patients were involved, 26 were male (93%) and two were female (7%). The median age of patients was 8.5 months. The median duration of cough was four weeks. The average length of hospital stay was (8.3 ± 3.9)days. The main clinical feature was wet cough in 28 cases, wet cough with wheezing was seen in 21 cases. The wet cough phase distribution was irregular in 21 cases. The crackles with wheeze (in 21 cases) was main signs of PBB. The percentage of CD3⁻ CD16⁺ 56⁺ cells increased in peripheral blood. The fiberoptic bronchoscopic manifestations of PBB were luminal mucosal edema. Eleven patients also had airway malacia. The neutrophil median in BALF was 0.2. The positive rate of bacterial culture of BALF was 36%. The main bacteria were Streptococcus pneumoniae (50%) and Haemophilus influenzae (30%). The main treatment for PBB patients included amoxycillin/clavulanate potassium and second-generation cephalosporins. The average duration of treatment was (17.3 ± 3.2)days, the prognosis was good.

CONCLUSIONPBB is common in male infants. Persistent wet cough with wheezing was the main characteristic of PBB. PBB is commonly accompanied by immune dysfunction and airway malacia, and the pathogens were Streptococcus pneumoniae and Haemophilus influenzae.

Bacterial Infections ; drug therapy ; pathology ; Bronchitis ; drug therapy ; microbiology ; pathology ; Bronchoalveolar Lavage Fluid ; Bronchoscopy ; Cough ; Female ; Haemophilus influenzae ; isolation & purification ; Humans ; Infant ; Male ; Respiratory Sounds ; Retrospective Studies ; Streptococcus pneumoniae ; isolation & purification

Cryptogenic stroke (CS) is a type of stroke that can not find the exact cause after using the standard clinical examination procedure of stroke. In recent years, many studies have shown that patent foramen ovale (PFO) is closely associated with CS, and its main pathogenesis is paradoxical embolism. In clinical practice, ultrasound is often used for PFO screening. In the context of PFO, the secondary prevention of CS includes drug therapy and PFO closure, but the choice of treatment is still controversial. Screening and evaluation of possible PFO will help to develop secondary prevention strategies for patients with CS, especially those who can benefit from PFO closure.