Objective:To study the mental health status of middle school students in Three-Gorges area of Chongqing in order to provide theory support for mental health education. Methods:1 014 middle school students in Three-Gorges area were tested by SCL-90. Results:The occurrent rate of mental problems with students was 19.8%,of which the first three factors were hostility,compulsiveness and interpersonal sensitivity. Girls’ overall score and mean scores of interpersonal sensitivity, depression,anxiety and phobic anxiety were significantly higher than boys’. The overall score and factor mean scores of senior high school students were significantly higher than those of junior high school students’ except hostility and phobic anxiety factors. The mean scores of interpersonal sensitivity,hostility and phobic anxiety factors were significantly higher than the counterparts in the norms of middle school students,while the mean scores of somatization and anxiety factors were significantly lower than the counterpart in the norms of middle school students. Conclusion:The mental health status of middle school students in Three-Gorges area of Chongqing is poor,and mental health education should be enhanced by all of us.

Aim To evaluate the inhibitory effect of panaxynol(PNN) on the proliferation of rat aortic smooth muscle cell(RASMC) and its mechanisms.Methods Cell proliferation was determined using cell count and -TdR incorporation test.Fura-3/AM and confocal were used to measure intracelluar free Ca~(2+) concentration.Expression of mitochondrial transcription factor 1(mtTF1) mRNA was tested by using RT-PR.Results PNN inhibited the RASMC proliferation and DNA synthesis induced by serum and PDGF-BB in a dose-dependent manner.9 ?mol?L~(-1) of PNN inhibited the increase of intracelluar free Ca~(2+) concentration induced by PDGF-BB.PDGF-BB upregulated the expression of mtTF1 mRNA,which could be suppressed by 3,9 ?mol?L~(-1) of PNN significantly.Conclusions PNN can inhibit RASMC proliferation significantly,which might be related to the decrease of intracellular free Ca~(2+) concentration and mtTF1 mRNA expression.

Objective:To construct an expression plasmid of siRNA against gene hIAP-2 and to assess its effect on breast cancer cell line MCF-7.Methods: A hIAP-2 siRNA plasmid was constructed with mU6pro vector containing U6 promotor and its RNAi effect on MCF-7 cells was detected by RT-PCR and Western blot.The effects of the plasmid on MCF-7 proliferation were analyzed by MTT assay,on the cell cycle by flow cytometry,and on the cell morphology by Hoechst staining.The activity of caspase-3 was determined with its substrate Ac-DEVD-pNA and the expression of some related proteins was detected by Western blot.Results: The hIAP-2 siRNA plasmid knocked down hIAP-2 expression in MCF-7 cells specifically and obviously,inhibited tumor cell proliferation and arrested the MCF-7 cells at G_(1) phase.After hIAP-2 gene was silenced,MCF-7 cells became multinuclear and had macronucleus;procaspase-3 was activated and its expression was increased.The protein levels of I?B?(p37) and p21~(waf1) increased,of NF-?B(p65) decreased and Cyt C remained unchanged.Conclusion: hIAP-2 in MCF-7 cells plays a crucial role in cell proliferation and cell cycle progression.Mitotic cell death might be the main way of the cell death;G_(1) arrest after DNA damage might be associated with the p21~(waf1) increase.

Objective: To study Blood Stasis Syndrome by molecular-biology in kidney-deficiency of diabetes pedigree.Methods: 18 members in diabetes pedigree were closed to make routine physical & chemical test and the macroscopic diagnosis of kidney-deficiency and blood stasis,3 diabetics with blood stasis syndrome and 2 healthy people in the same family,were selected to make 14000 genes chips hybrid test.Results: The pedigree maps and the comparative syndrome score tables of kidney-deficiency and blood stasis syndrome were obtained,and the 446 genes of different expressions,in particular,5 genes such as the TBXAS1,which was used to illustrate the possibility of effects on the blood stasis were also selected Conclusion: There was an exceeding preponderance of pedigree research method,which was used to search the macroscopic syndrome and the microscopic expressions of different genes.And it would help us to understand the relationship between the syndrome of kidney-deficiency & blood stasis diabetes and the genes of different expressions.

Objective TO Provide information for clinical diagnosis and study the mechanism of sexual abnormality . Methods 6 cases of sexual abnormality were detected with PCR amplification and DNA sequencing techniques.ResultsSRY gene was positive in 45,XO male and 46,XY female whose DNA sequence of conserved region had no mutation.SRY gene was negative in 46,Xxmale.Three46,XY males with sexual abnormality showed SRY positive.Conclusions The sexual abnormality of 45,Xomale was caused by SRY positive.Conclusions The sexual abnormality of 45,XOmale was caused by SRY gene translocation .Sexual reverse infemale was caused by gene conseved region mutation but in male it might be caused by sexual related gene mutation on chromasome.

Objective To investigate the relationship between reading speed and center scotoma in age-related macular degeneration.Methods 46 eyes of 46 age-related macular degeneration patients underwent reading speed and center threshold examination,and the relationship was analysed.Results The mean reading speed was 183?60words/min,and had significant relationship with the deepness of the center scotoma.Conclusions The reading speed in age-related macular degeneration patient was decreased,and it related to the deepness of the center scotoma.

Objective To study the affection of acanthopanacis biochemical capsule to drug abortion in clinics.Methods 600healthy pregnant women were divided into three groups randomly;200 group A,oral intake of acanthopanacis biochemical capsule after drug abortion,200 group B oral intake of Yimuwan after drug abortion,200 group C as control.Results The discharging rate of pregnant cyst within 3days were 84.62% in grpup A,63.64% in group B and58.33% ingroup C.Theoccurrence rate of incomplete abortion within 2weeke were 1.50%in group A,6.00% in group B and 91.67% in group C .The rate of vagina bleeding within 2 weeks were 9.50% in group A,97.50% in group B and 69.80% in group C.The rate of vagina bleeding over 1 month were 1.00% in group A,6.50% in group B and 7.50%in group C.The emergency operation due to great hemorrhage were 1 in A,7 in B and 9 in C.The uterus infection were 1 in A,13 in B and 15 in C.Conclusions Acanthopanacis biochemical capsule could increase the successful fate of dug abortion within 3 days and decrease the chance of inconplete abortion .It could shorten the tine of vagina bleeding and the happening of complication.

Objective:To analyze the reasons of complicated arrhythmia after resection of lung. Methods:The relation of arrhythmia with age, operation mode,preoperative lung function and postoperative blood gas of 562 cases of lung resection were analyzed. Results:There were 104 of 562 cases (18.5%) complicated with arrhythmia postoperatively. 38.77% of them were older than 70 years. 27.72% had operation of pneumonectomy.The parameters of FEV 1,MVV,FVC lower than 50% were 67.24%,46.53% and 67.93% respectively.Postoperative blood gas analysis of the 23 cases showed that Po 2 was lower than 60 mmHg,Pco 2 was higher than 45 mmHg,So 2 was lower than 96%.21 of them developed postoperative arrhythmia.After tracheotomy and immediately correcting anoxia,all the 21 patients recovered from arrhythmia within 3 days.4 patients died in this group(3.84%).Conclusion:Pneumonectomy,in patients older than 70,results in the parameters of FEV 1,MVV and FVC lower than 50%.Significant anoxia is the cause of arrhythmia.

Objective:To discuss the method of Video Assisted Thoracoscopy(VATS) in diagnosis and treatment of small peripheral lung neoplasm.Methods:A wedge-shaped excision with VATS had been performed on 20 patients with small peripheral lung neoplasm.The sample had been sent to pathology during surgery.Lobectomy was done with the malignant tumor.Results:14 cases(70%) were malignant in stage IA ,6 cases were benign.The mean follow-up time postoperatively was 24 months(12～36months),there was no relapse case.Conclusion:For those patients,the diameter of neoplasm is smaller than 15mm,and antibiotic treatment is ineffective for 2～3 weeks,VATS can be available for diagnosis and treatment.

Objective:To investigate the therapeutic effect of sequential blood purification on rescuing acute grave intoxication.Methods:Twenty eight patients with acute intoxication were treated by HP+CVVH during 2002 and compared them with thirty patients without hemopurgation from 1998 to 2002.Results:The group of hemopurgation was better than non-hemopurgation group in the time of revive,the rate of recovery and death,and the total dose of atropine.Conclusion:Hemopurgation has decisively effect on rescuing all kinds of intoxication with toxins and drugs.It could quickly ameliorate symptom,raise success rate and reduce mortality;Hp combining with CVVH are suitable for the patients with toxicsis who had cardiac and respiratory failure,brain edema,and dielectric disorder.