Objective:To construct an expression plasmid of siRNA against gene hIAP-2 and to assess its effect on breast cancer cell line MCF-7.Methods: A hIAP-2 siRNA plasmid was constructed with mU6pro vector containing U6 promotor and its RNAi effect on MCF-7 cells was detected by RT-PCR and Western blot.The effects of the plasmid on MCF-7 proliferation were analyzed by MTT assay,on the cell cycle by flow cytometry,and on the cell morphology by Hoechst staining.The activity of caspase-3 was determined with its substrate Ac-DEVD-pNA and the expression of some related proteins was detected by Western blot.Results: The hIAP-2 siRNA plasmid knocked down hIAP-2 expression in MCF-7 cells specifically and obviously,inhibited tumor cell proliferation and arrested the MCF-7 cells at G_(1) phase.After hIAP-2 gene was silenced,MCF-7 cells became multinuclear and had macronucleus;procaspase-3 was activated and its expression was increased.The protein levels of I?B?(p37) and p21~(waf1) increased,of NF-?B(p65) decreased and Cyt C remained unchanged.Conclusion: hIAP-2 in MCF-7 cells plays a crucial role in cell proliferation and cell cycle progression.Mitotic cell death might be the main way of the cell death;G_(1) arrest after DNA damage might be associated with the p21~(waf1) increase.

Objective To investigate the effects of different times of needle retention on cognitive function in patients with vascular cognitive impairment no dementia (VCIND). Methods One hundred and four VCIND patients were randomly allocated to group A of 35 cases, group B of 34 cases and group C of 35 cases. All the three groups received acupuncture plus medication. Of them, group A was given scalp acupuncture with 30 min retention of needles;group B, with 1 hr retention of needles;group C, with 10 hrs retention of needles. The Montreal Cognitive Assessment (MoCA) score and the ADL score were recorded in the three groups of patients before and after treatment. Results There were statistically significant pre-/post-treatment differences in the MoCA score and the ADL score in every group (P<0.05). There were statistically significant post-treatment differences in the MoCA score and the ADL score between group A or B and group C (P<0.05). Conclusions Acupuncture is an effective way to treat VCIND. It can improve cognitive function in the patients. Scalp acupuncture with 10 hrs retention of needles has a more marked effect.

With the development of global warming,urbanization,ageing of the population,it is paid more attention to the human health impacts on the frequent occurrence of heat wave and urban heat island effect.The study methods and advances in impacts of heat wave on human health were reviewed in the present paper.

The prevalence rate of diabetes is continuously increasing in China, and diabetes characterizes as high morbidity, low diagnostic rate, low cure rate, and low good control rate, which consequently resultes in the occurrence and progression of various diabetic complications and dramatically decreases the quality of life as well as the survival of diabetic patients.So it is crucial for clinician to screen diabetes early, make the diagnosis early, and start the treatment promptly.This article systemically summarizes the clinical manifestations, diagnostic criteria, typing, pathophysiology, complications, and management of diabetes.

Nephroblastoma is one of the most common solid tumours in children,and the gene expression is closely related to the tumorigenesis,development and prognosis.The treatment regimens have been constantly updated,which includes the preoperative chemotherapy,nephron-sparing surgery and targeted therapy of cancer stem cells,aiming to maintain the excellent survival for children being treated for Wilms tumor,while minimizing therapy-related toxicity.This paper reviews the gene expression and treatment on wilms' tumor.

Objective To evaluate the effects and security of single or combined sufentanil application on sedation and analgesia for mechanically ventilated patients in intensive care unit(ICU).Method A total of 160 ICU patients with mechanical ventilation were randomly allocated into four groups:sufentanil group,sufentanil plus midazolam group,sufentanil plus dexmedetomidine group and fentanyl group.Facial pain scale(FPS),Ramsay score and vital signs were employed to assess the effects of different therapies on organ functions and adverse reactions.Result After the treatment,FPS in all groups were significantly decreased and maintained around 3 points.Ramsay scores were significantly increased(P <0.001)and the score in group given sufentanil combined with midazolam or dexmedetomidine at the time points of30 min,1 h,6 h were even higher.Compared with the preoperative data,indicators of lung function(PaCO2and blood lactic acid),liver function(AST)and renal function(Cr)were decreased in all groups(P <0.05).Respiratory rate in the fentanyl group was significantly higher than the others.Total consumption ofsufentanil in group with combined sufentanil application was significantly lower.No significant differencesin adverse reactions were noticed among the groups(P >0.05).Conclusion For mechanically ventilatedpatients,sufentanil has efficient sedative and analgesic effects with less physiological interference and severe adverse reactions.Combined application is beneficial in decreasing total sufentanil consumption andinhibition to respiratory system,which is worthy of being spread.

Objective To discuss the effect of the PDCA cycle in enhancing the case care ability of nurses in order for nurses to adapt to the assessment model of cases tracking.Methods The training was carried out for enhancing nurses' ability of case care according to the PDCA cycle.Results The scores of the case care,the quality of care in wards and the patient satisfaction degree increased significantly after applying the PDCA cycle compared with those before.The aims of ensuring medical safety,improving the work quality,and increasing patient satisfaction were achieved.Conclusions Training nurses' ability of case care following the PDCA cycle can effectively improve the nurses' ability of case care,make them adapt to the case tracking assessment model,which is worthy of promotion.

Objective To explore the clinical efficacy of cetuximab combined FOLFOX chemotherapy on colon and colorectal cancer.Methods Forty patients with colon and colorectal cancer were randomly divided into observation group and control group,and 20 cases for each group.Patients in control group were treated with oxaliplatin + leucovorin + 5-fluorouracil,and in the observation group were given cetuximab (the first does was 400 mg/m2 at the first week,then the second week and follow weeks was 250 mg/m2 1 time/week for 6 week) plus the treatment as control group.The clinical efficacy,adverse effect and 1-year survival in the two groups were recorded.Results The total effective rate and clinical benefit rate in observation group were 60％ (12/20)and 85％ (17/20) respectively,which significantly higher than the control group (30％ (6/20) and 65 ％ (13/ 20),x2 =18.18,10.67,P ＜ 0.05).Skin rash rate in observation group was 55％ (11/20),which was significantly higher than that of control group (15％ (3/20),x2 =35.16,P ＜0.05).Half-year survival rate and 1-year survival rate of patients in observation group were 75％ (15/20) and 45％ (9/20) respectively,significantly higher than the control group (50％ (10/20) and 25％ (5/20),x2 =13.33,8.791,P ＜ 0.05)~ Conclusion Treatment scheme of Cetuximab combined FOLFOX chemotherapy is proved to be an effect of treating advanced colon and colorectal and be with the low adverse effect.

Systems biology is a new science of the 21st century, which resembles traditional Chinese medicine (TCM) in many aspects such as study method and design. Adoption of systems biology approach would do much help for exploring the scientific connotation of TCM syndrome and the modernization of Chinese herbal medicine. Its technological platforms, such as genomics, proteomics and metabonomics, provide powerful tools for the study of the essence of TCM syndrome and the function of herbal compound recipe. Compared with other molecular biological methods, such as genomics and proteomics, metabonomics method is more direct and more concise, especially for providing an effective method for the study of TCM therapy in preventing and treating kidney diseases.

[Objective]To evaluate the effects of mosapride combined with acupressure at selected time and psychotherapy on constipation-predominant of irritable bowel syndrome.[Methods]A total of 86 patients with constipation-predominant of irritable bowel syndrome(C-BS) randomly divided into two groups:the treatment group(n=43) and the control group(n=43). Every patient in the study was diagnosed C-BS according to Rome II criteria. Patients in control group took mosapride (5mg,tid) and in treatment group acupressure at selected time and psychotherapy were givn besides mosapride.[Results]The total effective rate of the treatment group was 86.0%,which was superior to that of the control group(60.5%)(P