Purpose:To investigate the effect of p53 gene transfection and 1,2∶5,6-dianhydro-3,4-diacetylgalactitol (DADAG) on the growth of hepatocellular carcinoma(HCC). Methods:The HCC line HLE that were transfected with plasmid pUHD10-3 recombined with wt-p53 gene by lipofectamine-mediated were cultured in medium containing DADAG. 3-(4,5-Dimethy-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium-bromid(MTT) technique was used to analyse the growth of HCC cells. Results:The growth of HCC cell line HLE were inhibited after being transfected with wt-p53 gene,and 67.68% of the cells were inhibited on the fourth day. 53.09% of HCC cell line HLE were inhibited when they were exposed to DADAG. When the HCC cell line HLE transfected with wt-p53 gene were exposed to DADAG,85.37% of the cells were inhibited. Conclusions:The inhibition efficiency of HCC was enhanced by the combination of DADAG and wt-p53 gene transfection.

Objective To investigate the Allelic and haplotype frequency distribution of seventeen short tandem repeat loci of Y chromosome in Heiyi Zhuang ethnic groups in Guangxi province.Methods Seventeen Y-STR loci,of which the template DNAs were extracted from blood samples of 184 unrelated male individuals in Heiyi Zhuang population,were amplified by using the AmpFlSTR YfilerTM.The PCR products were genotyped with ABI PRISM 310 genetic analyzer.Results The Gene diversity ranged from 0.4910 to 0.9727 at DYS456、DYS389Ⅰ、DYS390、DYS389Ⅱ、DYS458、DYS19、DYS385a\b、DYS393、DYS391、DYS439、DYS635、DYS392、Y-GATA-H4、DYS437、DYS438、DYS448.A total of 180 different haplotypes were observed,The haplotype diversity value calculated from all 17 loci was 0.99976.The significant difference of the allelic frequency distribution in Y-STR loci was found between Heiyi Zhuang population and other observed populations.Conclusion The 17th Y-STR loci in Heiyi Zhuang population of Guangxi province are highly affluent genetic polymorphic and can offer valuable genetic datas for paternity testing and paternal genetic lineages evolution.

Objective To investigate the modification effect of atmospheric temperature on outpatient visits caused by O₃ in Linzhi City. Methods The daily outpatient data, the daily O₃ concentration and daily meteorological data (including daily average temperature, average relative humidity, etc.) in Linzhi City from 2018 to 2019 were collected. The distributed lag non-liner-model (DLNM) was used to quantitatively evaluate the impact of O₃ in different temperature layers on the risk of outpatient visits. Results At low temperature layers, the cumulative relative risk (CRR) of total outpatient visits and non-injury outpatient visits increased by 53.8%（4.2% -126.9%) and 59.1%（5.8% -139.2%）for every 10 μg/m³ increase of O₃ concentration, respectively. The subgroup analysis showed that for every 10 μg/m³ increase of O₃ concentration at low temperature, the CRR of patients with circulatory diseases, men, women, and people being <14 years old and 14-65 years old increased by 152.1% (15.1% - 451.9%), 58.3% (2.1%-145.5%), 49.2% (3.0% -116.1%), 39.6% (2.5% - 90.3%), and 61% (0.8%-157.1%), respectively. Conclusion The average temperature may have a modifying effect on the outpatient visits caused by O₃ in Linzhi City. In general, the cumulative risk increases as the temperature decreases.