OBJECTIVE To study the constituent ratio of the Gram-negative bacteria of blood culture in recent 36 months and their resistance.METHODS Blood culture of patients in our hospital was performed by BacT/Alert 3D and the isolated bacteria were identified by API identified tests(API Inc.France) and additional antibiotics sensitivity test by Kirby-Bauer(K-B).RESULTS Of the 13766 specimens,1468(10.6%) were positive,including 734 Gram-negative bacteria strains(50.0%),476 Gram-positive bacteria strains(32.4%),233 fungi strains(15.9%),and 25 others strains(1.7%).CONCLUSIONS It is important and necessary to monitor the circumstance of the Gram-negative bacteria of blood culture.

OBJECTIVE: To determine whether the composite acoustic therapy is effective to treat tinnitus in patients with sudden deafness and to explore the mechanisms. METHOD: Ninety-six cases (96 ears) were divided into experimental group and control group, and all the patients underwent drug treatment. The patients in experimental group were given personalized composite acoustic therapy in the first 30 days, music therapy in next 31-90 days, however, the patients in control group were not given sound therapy. Additionally, pure tone audiogram, tinnitus pitch and loudness as well as questionnaires (including THI, VAS, and SAS) were conducted for each patient before treatment, at day 30 and day 90 posttreatment. RESULT: Eighty-nine patients (n = 47 for experimental group and n = 42 for control group) completed the trial. The results of day 30 posttreatment showed there were no significant differences in VAS and hearing recovery rate between these two groups, but THI and SAS showed significant differences. The results of day 90 posttreatment showed significant differences in VAS (P < 0.05), THI (P < 0.01) and SAS (P < 0.01), and no significant difference of hearing recovery rate was detected. The most significant changes of VAS appeared in the first 30 days of treatment. The hearing and tinnitus recovery in experimental group were better for the first 30 days of treatment than for the next 31-90 days, and similar results were obtaind in control group. (P < 0.01). CONCLUSION Composite acoustic treatment combined with drug therapy can improve the tinnitus and anxiety symptoms of patients with sudden deafness, and the effect on hearing recovery still need to be confirmed in further studies.
Acoustics ; Anxiety ; Hearing Loss, Sudden ; complications ; therapy ; Hearing Tests ; Humans ; Surveys and Questionnaires ; Tinnitus ; complications ; therapy ; Treatment Outcome

ObjectiveTo investigate the association of sleep disorders with the development and progression of nonalcoholic fatty liver disease （NAFLD）. MethodsA total of 1 868 participants from the health examination cohort and fatty liver cohort of Beijing Friendship Hospital from June 2022 to June 2023 were enrolled as subjects. Related data were collected from all subjects， including age， sex， education level， chronic medical history， and biochemical parameters， and all subjects completed Pittsburgh Sleep Quality Index （PSQI） scale independently. According to the diagnostic criteria， the subjects were divided into non-NAFLD group with 1 122 subjects and NAFLD group with 746 subjects， and according to the stage of progression， the patients in the NAFLD group were further divided into simple fatty liver group （SFL group with 624 subjects） and nonalcoholic steatohepatitis （NASH） group with 122 subjects. A one-way analysis of variance or the Kruskal-Wallis H test was used for comparison of continuous data between three groups. The chi-square test was used for comparison of categorical data between the three groups. The binary Logistic regression analysis was used to investigate the association between sleep factors and NAFLD， and the multinomial Logistic regression analysis was used to investigate the association between sleep factors and the different stages of NAFLD； two multivariate models were constructed for adjustment of potential confounding factors， i.e.， an age-sex adjustment model and a multivariate adjustment model， and the multivariate adjustment model adjusted the factors of age， sex， education level， smoking， diabetes， hypertension， body mass index （BMI）， triglyceride （TG）， and high-density lipoprotein cholesterol （HDL-C）. ResultsThere were significant differences in age， sex， BMI， education level， smoking， diabetes， hypertension， alanine aminotransferase， TG， and HDL-C between the non-NAFLD， SFL， and NASH groups （all P<0.05）. There were also significant differences between the three groups in the total score of PSQI scale and the proportion of subjects with a score of 0‍ ‍—‍ ‍3 points for the 7 sleep components （all P<0.05）. The multivariate adjustment model showed no significant association between sleep disorders and SFL， while long sleep latency （odds ratio ［OR］=4.04， 95% confidence interval ［CI］： 2.33‍ ‍—‍ ‍7.03， P<0.001）， short sleep duration （OR=3.53， 95%CI： 1.83‍ ‍—‍ ‍6.82， P<0.001）， and severe sleep disorders （OR=2.96， 95%CI： 1.48‍ ‍—‍ ‍5.93， P=0.002） were closely associated with the risk of NASH. ConclusionOverall sleep condition and its components of sleep disorders are not significantly associated with the simple fatty liver； however， long sleep latency， short sleep duration， and severe sleep disorders can increase the risk of NASH， which should be taken seriously in clinical practice.