To study the dynamic process of myocardial uptake of thiopentai in the isolated rabbit hearts. Method: Thiopental at doses of 500?mol, 1500?mol and 500?mol was given sequentially to the perfused rabbit heart in a total time of 15 min. The outflow concentration of thiopental was measured with high performance liquid chromatography and the left ventricular +dp/dtmax served as a effective parameter. Resuh: The disposition and elimination of thiopental can be best described hy a two-compartment open model. It can disposed into myocardium rapidly (T_(1/2)?=0.5?0.1 min), but elimination was relatively slow (T_(1/2)?=25.3?10.1 min). The transfer rate was slower from peripheral to central compartment than from central to peripheral compartment. The tbeoritical maximum depressant effect of thiopental on + dp/dt (Emax) was 19.0 4-11.2 kPa.s~(-1) corresponding to 1/10 E_0. Conclusion: The myocardial uptake of thiopental can be fitted to a two-compartment open model with rapid disposition and relative slow elimination process.

Objective : To investigate whether miR⁃19a interacts with insulin⁃like growth factor 2 receptors ( IGF⁃2R) in infantile hemangiomas (IHs) and affects the proliferation , migration , and adipogenesis of hemangioma stem cells (HemSCs) . Methods : HemSCs were isolated , screened and cultured from IH specimens. IGF⁃2R expression in HemSCs was identified using immunohistochemistry. HemSCs transfected with miR⁃19a mimics and inhibitors were subjected to CCK⁃8 , wound healing , Transwell , qRT⁃PCR , and Western blot analyses. Results : Compared with the control , the proliferation and migration rate of HemSCs treated with miR⁃19a inhibitors were significantly increased , and overexpression of miR⁃19a significantly inhibited IGF⁃2 induced cell migration and proliferation (P < 0. 05) . Conclusion MiR⁃19a may inhibit HemSCs proliferation , migration , and adipogenesis by targeting IGF⁃2R.