Objective To investigate the changes of hair amino acids contents in patients with chronic kidney disease at stage 1-4 (CKD 1-4) and the intervention effect of Hushen No.Ⅱ, thus to provide modern medical evidences for the theory of kidney having its manifestations in hair. Methods One hundred cases of patients suffering from CKD 1-4 were recruited and were randomly divided into control group and treatment group, 50 cases in each group. And twenty healthy volunteers were included in normal group. The treatment group and control group received conventional treatment, and the treatment group were additionally treated with Hushen No. Ⅱ orally, the treatment lasting 12 months. And then high performance liquid chromatography was used to test the changes of hair amino acids of the three groups before and after treatment. Results Compared with the normal group, 14 kinds of amino acids in the hair of the control group and the treatment group were significantly low ( P<0.05) before treatment. After treatment, 14 kinds of amino acids in the hair of the control group and the treatment group were significantly increased ( P<0.05) as compared with those before treatment, the increase of the treatment group being superior to the control group (P<0.05). Conclusion The abnormal change of 14 kinds of amino acids in the hair of patients with abnormal renal function may contribute to the modern medical material basis of the theory of kidney having its manifestations in hair.

s:Professor SHAO Zhao-di is a famous old TCM doctor. She has been engaged in clinical of TCM for a long time, with extensive acquaintance and experiences. This article discussed three cases of her to treat allergic purpura nephritis.