Objective To understand the epigenetic regulations of P16 during cellular replicative senescence and premature senescence induced by hydrogen peroxide of human embryonic lung fibroblasts(HEFs).Methods The normal HEFs were divided into young cells(22 PDL),mid-aged cells(35 PDL)and replicative senescent cells(49 PDL)during replicative senescence.The synchronously cultured 22 PDL HEFs were exposed for 2 h to 400?mol/L H_2O_2 at half confluence on daily basis.The procedure lasted for 4 consecutive days.And then the treated cells were cultured for another 7 days,called premature senescent cells.The mRNA level of P16 was detected by fluorescence quantitative PCR.The methylation level in the promoter region -846～-639 bp was observed by methylation-specific PCR(MSP).The histone modifications was detected by chromatin immunoprecipitation-QPCR assay,including acetylation for H3,H4 and methylation for H3(Lys4)and H4(Lys20).Results In the process of cellular senescence,the mRNA level of P16 decreased in mid-aged cells,but increased significantly in both replicative and premature senescent cells compared with that of young cells(P

OBJECTIVE:To ensure patients'rights of knowing the fact of medication.METHODS:The characteristics of medical care and the chief problems existed at present were analyzed.The significance and the necessity of ensuring patients'rights of knowing the fact in medication in hospital were discussed and some specific measures were proposed.RESULTS&CONCLUSION:Ensuring patients'rights of knowing the fact in medication embodies hospital's demand for"taking people as top priority",building a harmonious society,upgrading hospital's image and promoting rational drug use,which deserves great attention from the relevant hygiene authorities and hospital leaders.

Objective To observe effect of Yaotui Litongsan on nerve-related behavior of autograft of nucleus pulposus to cavum epidurale in rats.Methods Fifty normal Wistar rats were randomly divided into five geoups:the sham operated group (A),injury model group (B),Yaotongning treat group (C),high dose and low dose group (Yaotui Litongsan treat,D and E),10 rats in each group.Autogenous nucleus pulposus was removed from the coccygeal vertebral and placed into the back of L5～6 nerve roots of left cavum epidurale,making the non-compressive model with transplanted autogenous nucleus pulposus.The mechanical withdraw threshold and thermal withdraw latency were determined.Results The significant mechanical hyperalgesia was showed in the rats of B,C,D,E groups postoperatively,but it was lighter in C,D,E groups than in B group (P

Objective To study the therapeutic effect of Shengfuling on the healing of various skin defect of human body.Methods Sixty cases of various skin defects were randomly divided into the treated group and the control group,which were treated by Shengfuling and normal healing after debridement,respectively.The healing time of defect areas and skin injury degrees was observed.The post-healing skin quality(color,hardness,tension) of the two groups were compared.Results The treated group had better effect than the control group in skin healing velocity and post-healing skin quality(P

OBJECTIVE: To discuss the relationship between drug price policy and the medical market order in China. METHODS: Considering Chinese medical market order, problems in drug price policy of our country were analyzed. RESULTS & CONCLUSIONS:Drug price policy of China is the one of key factor for the current medical market disorder, the formulation of which should be based on national condition and under governmental control.

OBJECTIVE:To discuss the thoughts over the event of“armillarisin A”produced by Qiqihar No.2Pharmaceut ical Co.Ltd.METHODS:Citing the event of“armillarisin A”produced by Qiqihar No.2Pharmaceutical Co.Ltd.as example,we analyzed problems occurred in the process of production and management of pharmaceuticals and traced the root causes.RESULTS&CONCLUSIONS:Pharmaceutical enterprises should bear in mind their social responsibility and leave no loophole in the control and supervision of drugs,they should draw lessons from this event and prevent the similar event from happening again by working out effective measures to tighten controls on the quality of drug production,registering and approval,circulation,ADR reporting,etc.

OBJECTIVE: To investigate the possible role of Caspase 3 and Bcl - 2 in arsenic trioxide(As2 O3) - induced apoptosis of Namalwa cell line .METHODS: Cell apoptosis was assessed through agarose gel elecrophroresis and the double staining of Annexin V and propidium iodide(PI), of which fluorescent intensities were measured on flow cytometer.Namalwa cells were treated with As2 O3 and DEVD - CHO, a selective inhibitor of Caspase 3,and the changes of expression of Caspase 3 and Bcl - 2 were observed .RESULTS: As2 O3 up - regulated Caspase 3 protein and down - regulated Bcl - 2 protein. DEVD-CHO inhibited the apoptosis induced by As2O3. but it had no effect on down - regulation of Bcl - 2 protein induced by As2O3.CONCLUSION:The activation of Caspase 3 may play a role in mediating apoptosis induced by As2O3 in Namalwa cells.

OBJECTIVE:To investigate the preparation technic and method of quality control of Xinyoukexing tincture(XYKX) METHODS:To determine the content of Valaciclovir by spectrophotography,and podophyllotoxin by dual-wavelength spectrophotography RESULTS:In the range of 14 88～34 72?g/ml,the Valaciclovir concentration in XYKX was in direct proportion to the absorption at 310 4nm,C=44 5 905A+1 7 556,r=0 9 998 In the range of 17 50～50 12?g/ml,the podophyllotoxin concentration in XYKX was in direct proportion to the absorption at 292nm,C=97 7 981Ap292+1 3 614,r=0 9 999,AP292=As292-As325 CONCLUSION:The preparation technic of XYKX is simple and the method of quality control is feasible

The tadpole extract without large molecular protein(T-871) was prepared from dry tadpoles. This extract and RPMI1640 medium were mixed in the ratio of 1:50(V/V) to form T-871 medium. HeLa cells were cultured in the T-871 medium in order to study the possible mechanism of HeLa cell differentiation induced by tadpole extract. We found that there was a decreased acitivity of LDH which may be released through HeLa cell membrane in the 2 day's cultured T-871 medium. After 3 day's culture in the T-871 medium we found that on the HeLa cell membranes, Na-K-ATPase activity reduced and the content of Con A receptors increased. When these cells were analyzed by flow cytometry(FACS-420), the results indicated that HeLa cells accumulated in G_2 and M phases of the cell cycle and the influence on DNA and RNA content of the HeLa cells was insignificant. HeLa cells cultured by RPMI 1640 medium or T-871 medium for 3 days were inoculated into the dorsal hypodermis of 12 nude mice, respectively. Then after 24 hours this extract was injected to each nude mouse at dose of 0.3 ml each day. Our study showed that T-871 could result in decrease of tumor formation such as delay of tumor nodule appearance and reduce of tumor weight. This study suggested that the changes of the cell membrane may be caused by the tadpole extract. It may also reduce malignancy of the HeLa cells.

OBJECTIVE:To analyze the utilization of drugs in children with ventricular septal defect(VSD) undergoing operation.METHODS:The medical orders of 120 VSD children patients in our hospital in 2005 were analyzed retrospectively.RESULTS:The per capita drug cost was(3 067.20? 325.04) yuan,accounting for 9.52% of the total medical cost.Circulatory system drugs and antibiotics were the chief categories.Leading the first 3 places in terms of DDDs were cefuroxime,vecuronium bromide and furosemide,respectively.CONCUSION:The drug cost for VSD children patients was relatively low and reasonable.