1.Inhibitory effects of angiogenesis inhibitor TNP-470 on growth of a human malignant glioma cell line SHG-44 and its xenografted tumor
Linlin DU ; Xiuwu BIAN ; Yisheng CHEN ; Jingquan SHI
Journal of Third Military Medical University 2001;23(3):272-275
Objective To investigate the effects of TNP-470 on the growth of a human malignant glioma cell line SHG-44 in vivo and in vitro. Methods The colorimetric MTT assay, soft agar culture, flow cytometry,light and electron microscopy were used to determine the proliferation, the cloning efficiency, cell cycle and the morphological changes of SHG-44 cells as well as the growth of its xenografted tumor. Results TNP-470 (20~2 000 ng/ml) significantly inhibited the proliferation of SHG-44 cells in vitro (the 50% inhibitory concentration was 200 ng/ml). Cloning efficiency reduced obviously. The number of cells in G0/G1 phase increased, while that in S, G2/M phases decreased significantly. Weight and volume of xenografted tumors treated with TNP-470 (30 mg/kg, injected subcutaneously every other day) reduced notably. Furthermore, there were necrotic area and apoptosis in the tumor. No severe side effect of TNP-470 was found in this study. Conclusion The inhibitory effect of TNP-470 on the growth of SHG-44 cells correlates with its functions of regulating cell cycle and inducing apoptosis, which suggests that the angiogenesis inhibitor TNP-470 has strong inhibitory effect on human malignant gliomas.
2.Models and mechanisms for international cooperation and exchange in the disciplines development
Xinsheng ZHANG ; Kui AN ; Linlin BIAN ; Chunwei PANG ; Xunming JI
Chinese Journal of Medical Science Research Management 2014;27(6):665-666,669
International cooperation and exchanges plays an important role in the disciplines development in hospital,and can indirectly improve the overall capacity and quality of healthcare..This paper draw on 10 years of international cooperation and exchange in the practice of discipline development,,analyzed working patterns and mechanisms to strengthen international scientific cooperation.
3.Research status on quality of life in patients with aneurysmal subarachnoid hemorrhage
Linlin ZHU ; Rui BIAN ; Chao CHENG ; Hao YUAN ; Hui WANG ; Yousong XU
Chinese Journal of Postgraduates of Medicine 2016;39(6):570-573
Aneurysmal subarachnoid hemorrhage accounted for 70% of subarachnoid hemorrhage. The high fatality and disability rate affected patients′ quality of life. Aneurysmal subarachnoid hemorrhage should be considered as a chronic cerebrovascular disease, and it is very important to take early intervention and strengthen the secondary prevention to the patients with risk factors for disease. This paper reviewed the research status on quality of life of patients with aneurysmal subarachnoid hemorrhage and its influencing factors, in order to provide a reference for neurological physicians.
4. The clinical significance of invariant natural killer T cells in patients with human immunodeficiency virus infection
Meiyin ZOU ; Yongwu LING ; Zhaolian BIAN ; Linlin JU ; Meihua WANG ; Lihua GU
Chinese Journal of Infectious Diseases 2018;36(4):222-226
Objective:
To explore the changes of the peripheral invariant natural killer T (iNKT) cells in patients with human immunodeficiency virus (HIV) infection.
Methods:
A total of 101 patients with HIV infection including 52 asymptomatic patients and 49 acquired immunodeficiency syndrome (AIDS) patients were enrolled in the study from June 2016 to July 2017. Flow cytometry was used to detect iNKT cells, CD4+ T cells and CD8+ T cells, and the relationship among them and HIV RNA was studied. At same time, 12 healthy persons were enrolled as control group.
5.Practice and reflection on international cooperation and exchange in hospital discipline construction
Kuo LIANG ; Linlin BIAN ; Xinsheng ZHANG ; Yan YIN ; Dongyue SUN ; Jingfang YANG ; Xiuhai GUO ; Xunming JI
Chinese Journal of Medical Science Research Management 2018;31(6):461-464
Objective Discipline construction is the core for the development of a hospital and international cooperation and exchange play an important role in promoting discipline construction.This paper summarized the experiences of carrying out international cooperation and exchange in the hospital discipline construction.Methods Analyzed the exploration,practice and its effect in promotion of hospital discipline construction regarding to international cooperation in recent years.Results The international cooperation and exchange have effectively promoted the development of hospital disciplines,for instance,introduction and training of talents,organizing and holding of high-level international conferences,establishment of international academic journals and the joint construction of academic platforms.Conclusions In accordance with the requirements of discipline development strategy,the hospital can improve the overall level of discipline construction rapidly by carrying out all-dimensional cooperation with international top medical institutions.
6.Role of dermoscopy in assessing vitiligo activity
Chao FU ; Jiaona WU ; Wenchao LANG ; Fei GAO ; Guiye NIU ; Peiwen BIAN ; Minhong GAO ; Xiaoqing SI ; Linlin XIN
Chinese Journal of Dermatology 2022;55(3):268-271
With in-depth research and development of dermoscopy, the dermoscopic features including perifollicular pigments, perilesional pigments, pigment network structure, satellite phenomenon and "tapioca sago" appearance, micro-Koebner phenomenon and comet tail-like phenomenon have provided a basis for the evaluation of vitiligo activity. This review summarizes progress in the evaluation of vitiligo activity with dermoscopy in recent years, aiming to promote the application of dermoscopy in the assessment of vitiligo activity.
7.Preliminary exploration and practical effect on the refinement of the process management of the nursing special project in comprehensive tertiary hospital
Kuo LIANG ; Xi CHEN ; Binru HAN ; Linlin BIAN ; Jingfang YANG ; Yan YIN ; Xinsheng ZHANG ; Xinya WANG ; Zhichen YIN ; Xiuhai GUO
Chinese Journal of Medical Science Research Management 2023;36(5):356-359
Objective:To analyze the preliminary explorations and practical effects of strengthening the process management of nursing special projects in a tertiary general hospital, so as to explore the refined management strategy of hospital projects and provide reference for improving the management level of the research project.Methods:This article takes a series of refined measures taken by the hospital in the process management of nursing special projects from August 2016 to August 2021 as the research example, including setting up subject directions around key disciplines, setting application conditions at different levels, standardizing selection procedures, strengthening mid-term assessments, strictly controlling final acceptance, establishing integrity files, and promoting the transformation of scientific achievements, etc. and these measures are deeply analyzed and discussed.Results:Through a series of measures, the hospital nursing team has significantly improved in terms of research paper publication, patent authorization, approval of projects above the bureau level, and nursing specialties ranking of Chinese hospital Science and Technology Evaluation Metrics (STEM) in the past six years.Conclusions:By strengthening the process management of nursing special research project, the construction level of nursing disciplines has been significantly improved, and the development of nursing disciplines has been greatly promoted.
8.Effect of ELMOD2 on malignant biological behaviors of gastric cancer MGC803 cells
QIAO Linlin ; CHEN Liang ; BIAN Yue ; SUN Xiuju
Chinese Journal of Cancer Biotherapy 2019;26(6):632-637
Objective: To investigate the effect of ELMOD2 over-expression on the malignant biological behaviors of gastric cancer MGC803 cells, and to study its related molecular mechanism. Methods: GV141-ELMOD2 expression vector was transfected into human gastric cancer MGC803 cells. The mRNA and protein expressions of ELMOD2 were detected by Real-time fluorescent quantitative PCR and WB, respectively. The cell proliferation ability was detected by CCK-8 method. Apoptosis rate was detected by flow cytometry. The cell migration ability was detected by Transwell method. The protein expressions of PCNA, BAX and Bcl-2 and Vimentin were detected by WB. Results:After transfection of ELMOD2 expression vector, the mRNAand protein expressions of ELMOD2 were significantly increased in MGC803 cells (P<0.05). Further studies showed that over-expression of ELMOD2 increased the proliferation and migration ability but reduced the apoptosis rate of MGC803 cells significantly (P<0.05 or P<0.01). The protein levels of PCNA, Vimentin and Bcl-2 in MGC803 cells increased, while the protein level of BAX decreased (P<0.05 or P<0.01). Conclusion: Over-expression of ELMOD2 can promote the proliferation and migration of MGC803 cells and inhibit cell apoptosis. These effects may be achieved by increasing the protein level of PCNA, Vimentin and Bcl-2, and reducing the protein level of BAX.
9.CHINA PH ARMACY Volume 33,Number 6,March 30,2022 Semimonthly Table of Contents Guideline for the evaluation of medicine list in Chinese med ical instituti ons
Hui CHEN ; Guangquan SU ; Xiao LIU ; Fangyuan TIAN ; Yingyun GUAN ; Ngting Ti CHEN ; Yingnan ZHAO ; Linlin LIU ; Xiaolan BIAN ; Ting XU ; Xiaoyu LI ; Ong Aiz SHEN ; Lihong LIU
China Pharmacy 2022;33(6):641-652
In order to improve the management level of medicine list of medical institutions in China ,and help medical institutions build a medicine list of medical institutions with reasonable drug use structure ,standardized adjustment procedures , convenient operation and application and scientific evaluation methods ,so as to meet the needs of clinical rational drug use to the greatest extent ,with the support of the Pharmaceutical Care Professional Committee of the Chinese Pharmaceutical Association , China-Japan Friendship Hospital and the First Affiliated Hospital of University of Science and Technology of China (Anhui Provincial Hospital )jointly initiate and complete Guideline for the Evaluation of Medicine List in Chinese Medical Institutions jointly with a number of medical institutions. In strict accordance with the methodological requirements of World Health Organization standard guidelines ,based on the Delphi method ,the guideline formulation working group has constructed the quality evaluation index system and quantitative scoring table of medicine list management in medical institutions from the 5 dimensions of organization and management ,structure,adjustment,application and e valuation of the list. It is used to help medical institutions evaluate the quality of their medicine list management ,so asto play a positive role in the fine management of medicine list in medical institutions.