OBJECTIVE To investigate the incidence of nosocomial infection (NI) in our hospital,analyze the related factors,and provide theoretical basis for better prevention and control of NI. METHODS A total of 19 203 inpatients from Dec 2006 to Nov 2007 were retrospectively analyzed,of which 387 inpatients suffering from NI. RESULTS The rate of NI was 2.02%,the highest infected rate was in ICU (24.32%). The main infection site was respiratory tract (57.58%). The species were predominated by Gram-negatives (52.00%). CONCLUSIONS The incidence of NI is connected with factors such as age,underlying diseases,invasive operation and the misusage of antibiotics.