Objective To discuss the effects of high glucose on human mesangial cells (HMC)Wnt-β-catenin signaling pathway,and the intervention of rhein on it.Methods High concentration of glucose (30 mmol/L) combined with different concentrations of rhein were used to intervene cultured human mesangial cells.The activity of mesangial cell proliferation after all the interventions was examined by MTT measurement.Total Wnt、β-catenin RNA was detected By RT-PCR in normal mesangial cells and cells intervened by high glucose and Rhein.Results ①Inhibition effect to human mesangial cells:compared with NG group for 24 h,48 h and 72 h (OD values were 0.169± 0.051,0.228±0.074,0.285±0.075),human mesangial cells proliferation in HG group(OD values were 0.307± 0.074,0.507 ±0.038,0.711±0.075),HG+R1 group(OD value were 0.241± 0.027,0.334±0.015,0.499±0.063),HG+R2 group (OD value were 0.244±0.081,0.386±0.033,0.531±0.011),and HG+R3 group(OD value were 0.277±0.036,0.407± 0.057,0.594±0.042) were iucreased significantly (P＜0.05、P＜0.01) ; Compared with HG group for 24 h,48 h and 72 h,the HG+R1 group,the HG+R2 group and the HG+ R3 group showed a downward trend at 24 h,but not significantly; but the trend decreased significantly at 48 h and 72 h,and the performance expressed a time,concentration-dependent (P＜0.05,P＜0.01).② In the normal state,the mesangial cells expressed s certain amount of Wnt and β-catenin,when they were stimulated by high glucose,the expression of Wnt、β-catenin in mRNA increased (P＜0.05) ; while the expression of them was significantly reduced in high glucose-induced mesangial cells in HG+R1 group,HG+R2 group and HG+R3 group(P＜0.05).Conclusion Rhein may inhibit the proliferation of high glucose-induced mesangial cells through the Wnt and β-catenin gene expression.

Objective To explore the value of color Doppler ultrasonography(CDU)as preoperative diagnosing and postoperative monitoring in patients with stenosis of subclavian or innominate artery(SIA)before and after percutaneous transluminal angioplasty and stent(PTAS).Methods A total of 45 patients with stenosis of SIA were selected.Their extracranial vertebral artery and subclavian artery were observed with CDU.In 36 patients with one-sided stenosis of SIA.blood flow direction and spectrum of extracranial vertebraI artery were analyzed.Subclavian steal grade confirmed by spectrum change of vertebral artery was compared with the stenosis grade on quantitative angiography(QA).Among 45 patients with stenosis of SIA,PTAS was performed successfully in 36 patients(36 stents implanted).After stents implanted, hemodynamie parameters such as peak systolic velocity(PSV)in stent, follow-up velocity ratio in stem(VR)were measured.Restenosis in stent were observed.Results In 36 patients with one-sided stenosis of SIA,the ultrasonographic steal grade correlated with the QA stenosis grade significantly(r=0.752,P<0.01).Among 36 patients with stent implanted,follow up 8-12 months, 4 patients with restenosis in stent were found.Compared with the patients without restenosis in stent,PSV and VR in stent increased significantly(P<0.01).Conclusions As a fast and non-invasive imaging technique,CDU could allow diagnosis of stenosis of SIA before PTAS,and detection of restenosis after PTAS.

OBJECTIVE To study the pathogenic bacteria and its resistance to antibiotics in chronic suppurative otitis media. METHODS Secretion from 132 cases with chronic suppurative otitis media were cultured. Drug sensitivity tests were done to separate pathogenic bacteria. RESULTS 152 strains pathogenic bacteria were isolated from middle ear secretion. The main pathogenic bacteria were staphylococcus aureus (49 strains), pseudomonas aeruginosa (25 strains) and fungus (5 strains). Staphylococcus aureus was sensitive to fluorquinolones, and the sensitivity rate was 79.6 %. Pseudomonas aeruginosa was sensitive to fluoroquinolones and floxacin, and the sensitivity rates were 84 % and 64 %. CONCLUSION Staphylococcus aureus and pseudomonas aeruginosa are main pathogens of chronic suppurative otitis media in our region. Both of them are sensitive to fluorquinolones. Meanwhile, pseudomonas aeruginosa is also sensitive to ceftazidime of the third cephalosporins. So doctors should choose the sensitive antibiotics for treatment of the chronic suppurative otitis media, that may avoid the bacteria with drug resistance come into being. The fungus can not be neglected as the pathogen in chronic suppurative otitis media.

The association of interleukin 6 receptor ( IL-6R ) gene - 183 A/G ( rs4845617 ) and Asp358 Ala (rs8192284 A/C) polymorphisms with metabolic syndrome was investigated in Chinese Han population.The result showed that the frequencies of AA genotype and A allele were higher in patients with metabolic syndrome ( MS ) than those in healthy subjects ( P＜0.05 or P＜0.01 ).The risk of MS in patients with A allele was 1.643 folds of that with allele C(95％ CI 1.163-2.320,P＜0.01 ).No differences were found in the genotype and allele frequencies of -183A/G between two groups ( P＞0.05 ).The Asp358Ala polymorphism of IL-6R was significantly associated with MS in Chinese Han population.

OBJECTIVETo describe the intake of fruit and vegetables among employed migrant population aged 18 to 59 year-olds in China.

METHODSData from the Migrant Population Survey related to China Chronic Disease and Risk Factor Surveillance that conducted in 170 counties/districts in 31 provinces, 2012, was used. Information on non-communicable diseases and related risk factors among migrant population were collected through face-to-face questionnaire interview, physical measurement and lab tests. A total of 48 704 subjects aged 18 to 59 years old were included in our study. Sample was standardized by age and sex. Information on average daily fruit and vegetables intake, prevalence of low fruit and vegetables intake, grouped by sex, age, industries, and education level were analyzed.

RESULTSThe average daily intakes of vegetables and fruits were 353.7 (95%CI:351.3-356.2) g and 125.1 (95%CI:123.4-126.9) g respectively, among the employed migrant population aged 18-59 years old in China. Prevalence of low fruit and vegetables intake was 44.1% (95% CI:43.5%-44.6% ) among employed migrant population, 46.2% (95% CI: 45.5%-47.0%)for males and 41.2% (95% CI:40.3%-42.0%)for females (χ(2) = 82.19, P < 0.05). Among different professions, the prevalence of low fruit and vegetables intake was the highest among people working in accommodation and restaurants (46.2%, 95%CI:45.0%-47.3%) while the lowest seen among those working in social services (42.5%, 95%CI:41.4%-43.7%,χ(2) = 15.81, P < 0.05). The prevalence of low fruit and vegetables intake showed a decrease along with the increase of education levels (χ(2) = 22.29, P < 0.05).

CONCLUSIONIn 2012, more than 40% of the employed migrant population aged 18 to 59 years old in China had low fruit and vegetables intake. Being male and with low education level were risk factors linked with the higher prevalence of low fruit and vegetables intake.

Adolescent ; Adult ; China ; Diet ; statistics & numerical data ; Employment ; statistics & numerical data ; Female ; Fruit ; Humans ; Male ; Middle Aged ; Prevalence ; Risk Factors ; Surveys and Questionnaires ; Transients and Migrants ; statistics & numerical data ; Vegetables ; Young Adult