1.Pulsed-field gel electrophoresis (PFGE)molecular subtyping and drug resistance of Salmonella enteritidiss trains isolated in Longyan city
Qianjin CHEN ; Chunyuan CAO ; Meihua LI ; Haibin CHEN ; Linhong LIAO ; Chunrong HE ; Jianchao JIN
Chinese Journal of Microbiology and Immunology 2017;37(7):534-539
Objective To analyze the pulse-field gel electrophoresis (PFGE) patterns and antibiotic resistance of Salmonella enteritidisstrains isolated in Longyan city in order to provide reference for effective control, clinical diagnosis and treatment of outbreaks of food-borne diseases.Methods Seventy-seven Salmonella enteritidis strains isolated from food poisoning cases, livestock and poultry meat samples collected for food safety risk monitoring and sporadic cases in 2014 and 2016 were analyzed by PFGE.Antibiotic susceptibility testing was carried out to determine the minimum inhibitory concentrations (MIC) of 16 common antibiotics to 57 strains.Results Seventeen PFGE types were identified among the 77 Salmonella strains including three predominant types (P4, P6 and P8) and were grouped into three major pulsotypes (Cluster1-3) at 90% similarity level.The predominant types causing outbreaks of Salmonella enteritidis infection were P8, P6, P4 and P14, which were responsible for five cases of food poisoning.These isolated strains were highly resistant to nalidixic acid (93%), followed by resistance towards ampicillin (74%) and streptomycin (54%).Forty-one strains were resistant to three or more antibiotics (71.93%).Conclusion The PFGE types of Salmonella enteritidis strains isolated in Longyan city exhibit polymorphism and are clustered into three major pulsotypes.PFGE molecular typing can provide early-warning of sporadic outbreaks and help to identify the sources of infections in Salmonella enteritidis-related food poisoning.Attentions should be paid to multidrug resistant Salmonella enteritidis.Surveillance for multidrug resistant strains and usage of antibiotics should be strengthened.
2.A flexion loading system for knee biomechanics research.
Aijing SONG ; Qingfeng LIAO ; Xiaofang LI ; Linhong DENG
Journal of Biomedical Engineering 2012;29(4):710-715
It is important to design and build a kinetic loading system for flexing movement of knee joint to study knee biomechanics. The system reported here includes driving device, control device, and flexion angle determination imaging system. The driving device was constructed with a stepper motor and a mechanical transmission with a serried of clamps, shanks and so on, and the driving device was controlled by the control device with micro-control unit, a computer and the serial 232. While the knee joint was driven to move by the stepper motor, the flexion angle of the knee was determined using imaging-based techniques. The system achieved accurate loading and control of speed, extent and duration of knee flexion, as well as fast and non-contract determination of flexion angle during knee flexing movement. The system is simple to build, easy to operate, highly accurate and reliable and it provides an important tool for the study of knee biomechanics, and potentially provides a tool for helping patients of knee surgery during their post operation recovery training.
Biomechanical Phenomena
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Equipment Design
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Humans
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Knee Joint
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physiology
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physiopathology
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Microcomputers
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Orthotic Devices
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Range of Motion, Articular
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physiology
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Stress, Mechanical
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Weight-Bearing
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physiology
3. Molecular typing, virulence genes and drug resistance of Salmonella typhimurium in Longyan city
Qianjin CHEN ; Meihua LI ; Chunyuan CAO ; Linhong LIAO ; Haibin CHEN ; Xiaodong CHEN
Chinese Journal of Microbiology and Immunology 2019;39(11):805-811
Objective:
To analyze the molecular typing characteristics by pulsed field gel electrophoresis (PFGE), drug resistance and virulence genes of