BACKGROUND:There are less studies on static magnetic field, and its biological effects are stil unclear. OBJECTIVE:To investigate the static magnetic field effect on the secretion of nerve growth factor from Schwann cel s. METHODS:Schwann cel s were randomly assigned into three groups:0.05 mT sciatic magnetic field group, 0.1 mT sciatic magnetic field group and control group. At 2 days of inoculation, the cel s were exposed to the sciatic magnetic field, 12 hours per day. Cel s in the control group were not exposed to the sciatic magnetic field. After 6 days of exposure, RT-PCR method was used to detect nerve growth factor mRNA expression in Schwann cel s, and ELISA was adopted to detect the level of nerve growth factor secreted from Schwann cel s. RESULTS AND CONCLUSION:The mRNA expression and secreted level of nerve growth factors in the culture supernatant were significantly higher in the two sciatic magnetic field groups than the control group (P<0.05), especial y in the 0.1 mT group. However, there was no difference between the 0.05 mT and 0.1 mT groups (P>0.05). These findings indicate that the static magnetic field with certain intensity can promote the secretion of nerve growth factors from Schwann cel s.

From the perspective of both doctors and patients, the questionnaire survey was used in 1080 medi-cal workers and patients in 12 hospitals in Shandong province.They found that is extremely development trend to the doctor-patient relationship, doctor-patient trust evaluation is relevant, doctor-patient both pay attention to the influence factors of the government and society.Both doctors in the building of doctor-patient trust, is atta-ched importance to strengthen the construction of medical ethics of medical personnel, continue to deepen the re-form of the medical system and government regulatory functions into full play.

Objective To assess the application of spiral CT angiography in the diagnosis of Takayasu arteritis.Methods Vascular imaging features of three-dimensional CT angiography of 56 patients with Takayasu arteritis were analyzed retrospectively.Results The vascular imaging of all the patients were qualified for diagnosis.A total of 175 arteries were involved,including subclavian arteries (36.00%,63/175) with a high percentage of the left subclavian arteries (24.00%,42/175) ,common carotid arteries (29.71%,52/175) and renal arteries (10.86%,19/175) .The affected artery wall had varying degrees of concentric or eccentric thickening,and stenosis or occlusion of lumen was observed.Conclusion Threedimensional spiral CT angiography can make clear diagnosis of Takayasu arteritis,including the scope,extent and nature,and thus is worthy of clinical application.

Objective To evaluate the magneto-heating effect and cytotoxicity of the carbonyl iron powder as a feasibility for arterial embolization hyperthermia. Methods Different doses of carbonyl iron powder suspensions were prepared in vitro(10 mg/ml,100%;10 mg/ml,50%;or 64 g/L phenol solutions),and heated for 20 minutes in an alternating magnetic field(49.9,79.9,and 110.2 Oe).The influences of the doses of suspensions and currency of magnetic field on the heating effect were observed.Meanwhile,mouse fibroblast L-929 cells were cultured with the suspensions for 2,4,or 7 days.The morphology and relative growth rate(RGR) of the cells were determined by microscopy and MTT assay.The cytotoxicity of the suspensions was then classified. Results The heating ability of the carbonyl iron powder increased with the suspension concentration and the strength of the magnetic field.A optimal therapeutic temperature was achieved at 110.2 Oe with 60 mg/ml carbonyl iron powder suspension.The L-929 cells showed normal morphology after been treated by the carbonyl iron powder(10 mg/ml 100% solution and 50% solution) for 2,4 and 7 days with the 0-1 degree cytotoxicity.Conclusion The carbonyl iron powder has good heating effect under the alternating magnetic field,and is compatible with the tested cells.

Aspirin has demonstrated its efficacy in the prevention of adverse events related to cardiovascular disease, and lots of epidemiological studies suggest that it also can be used to prevent colorectal cancer and to decrease its recurrence after curative resection.The guideline published by USPSTF recently first time affirms the overall health benefit of aspirin in colorectal cancer prevention.Aspirin could prevent the colorectal cancer by the cyclooxygenase-dependent mechanisms and the COX-independent mechanisms.In this review, we will summarize some results of related researches and discuss the possible mechanisms.

Objective To investigate the predictive value of peripheral leukocyte and its subtypes count on cerebral-cardiovascular events in patients with unstable angina pectoris.Methods From December 2000 to January 2003,141 patients(aged 41-84 years) with unstable angina pectoris referred for coronary angiography were enrolled and their peripheral leukocyte and its subtypes were counted.The major adverse cerebral-cardiovascular events rate of these patients during 46.2?6.6 months of follow-up were assessed.Results A total of 55 cerebral-cardiovascular nonfatal events and 8 deaths were observed.After adjusting the risk factors of diabetes mellitus,smoking,hypertension,hyperlipidemia,prior myocardial infarction,family history,and blood fibrinogen and uric acid level,leukocyte count was positively associated with cerebral-cardiovascular events(adjusted relative risk 1.31,95% CI 1.15 to 1.49).The monocyte count in patients with cerebral-cardiovascular events was higher than that in patients without cardiovascular events(P

Objective To explore the clinical and serological features of patients with pneumomdiastinum (PNM) and dermatomyositis associated interstitial lung disease (DM-ILD).Methods A total of 145 polymyositis/dermatomyositis (PM/DM) patients hospitalized in our department from March,2010 to December,2012 were recruited.The sera,clinical and laboratory data were collected.Anti-aminoacyl-tRNA synthetase (anti-ARS) and anti-melanoma differentiation-associated gene 5 (MDA5) antibodies were detected by RNA-immunoprecipitation (RNA-IP) and ELISA,respectively.Anti-NXP2,anti-TIF1γ,anti-SRP,anti-SAE and antiMi2 antibodies were detected by immunoprecipitation-Western blotting.Variables were compared between DM patients with and without PNM,as well as between DM-ILD patients with and without PNM.Chi square test,Fisher's exact test,t test,Mann-Whitney U test and a multivariate logistic regression model were used for statistical analysis.Results A total of 11 DM patients (10 patients positive for anti-MDA5 and 1 patient positive for anti-Mi2) developed spontaneous PNM.No PM patient developed PNM.No differences of sex,age at the onset of DM,serum ferritin levels and C reactive protein (CRP) levels was observed between DM patients with and without PNM.Compared with DM patients without PNM,DM patients with PNM had significantly higher frequencies of rapidly progressive ILD (RP-ILD) (63.6％ vs 24.4％,x2=7.25,P=0.01),anti-MDA5 antibodies (90.9％ vs 52.4％,x2=5.86,P=0.02),clinically amyopathic DM (CADM) (63.6％ vs 22.0％,x2=8.57,P=0.007) and cutaneous ulcers (36.4％ vs 11％,x2=5.20,P=0.04),but significantly lower creatine kinase (CK) levels [58.5 (30.5,394.3) U/L vs 284 (73.0,917.0) U/L,t=207.5,P=0.04].The logistic multivariate analysis indicated that cutaneous ulcer was the only independent risk factor for the occurrence of PNM in DM [OR=5.98,95％ confidence interval (CI) (1.12,31.98),P=0.037].In the comparisons between DM-ILD patients with and without PNM,a higher frequency of CADM was observed in the PNM group (63.6％ vs 27.9％,x2=5.37,P=0.03),but no significant difference of the frequencies of RP-ILD,anti-MDA5 antibodies,cutaneous ulcers and CK levels was found in these two groups.All patients with PNM were treated with corticosteroids and immunosuppressants.During the follow-up period,6 patients died of respiratory failure.Conclusion Spontaneous PNM is a refractory complication with poor prognosis,and tends to occur in DM patients with RP-ILD,anti-MDA5 antibody,CADM diagnosis and low CK level,especially in patients with cutaneous ulcers.

At present, drug safety has gradually become the focus of world attention, and the study and control of related sub-stances is one of the key elements for drug safety. The national drug standards are also gradually increased the control requirements for related substances. Therefore, the detection and control of related substances are extremely important in the safety of drugs. By summa-rizing the analysis methods for related substances in drugsand their application , the paper alms to provide references for quality control of drugs.

Objective To compare the micromeritics properties and external dissolution rates of Sanhuang Powder in different particle sizes;To provide references for its direct use and application as raw materials for TCM preparation.Methods Particle size, bulk density, tap density, angle of repose, and hygroscopicity ofSanhuang micro-powder and common powder were investigated and evaluated. External dissolution rates ofSanhuang micro-powder and common powder were detected by ultraviolet spectrophotometry.Results The flowability of bothSanhuang micro-powder and common powder were not very well. With the sizes decreasing, the hygroscopicity of micro-powder became stronger. The external dissolution ofSanhuang micro-powder was more sufficient and much more quickly than common powder.Conclusion Properties ofSanhuang micro-powder and common powder are obviously different.Sanhuang micro-powder has stronger hygroscopicity and worse flowability compared with common powder. However, external dissolution ofSanhuang micro-powder is more sufficient and much more quickly than common powder. WhenSanhuang micro-powder is used directly and used as raw materials for TCM preparation, much more discretion should be considered.

Objective To discuss the teaching outcomes of project draft design combined with PBL teaching method in TCM pharmaceutics technology.Methods Fifty students majoring in 2009 TCM Pharmaceutics Technolgy were divided into A and B groups. 25 students in group A were set as experimental group by using project draft design combined with PBL teaching method, while 25 students in group B were set as control group by using traditional teaching method. This study evaluated the effects of teaching methods through questionnaire and score analysis of the two groups.Results Students taught by project draft design combined with PBL teaching method generally believed that this teaching method can stimulate their learning interest, improve their ability of locating problems, solving problems, and comprehensively applying knowledge and enhance test scores.Conclusion Project draft design combined with PBL teaching method shows better teaching outcomes than traditional teaching method, which can cultivate students’ all-round ability and comprehensive quality.