Tumor markers lay the foundation of early diagnosis for hepatocellular carcinoma (HCC).Besides alpha-fetoprotein, glypican 3 is confirmed to be a novel tumor marker for HCC with high specificity and sensitivity, especially at immediately early stage of small carcinoma. Glypican 3 has high value of clinical application and may bring new hope for precise diagnosis of HCC.

The human sclera accounts for 95％ of the surface of the eyeball,providing ample contact area which is suitable for targeted trans-scleral ocular drug delivery.Currently there are several tans-scleral sustained-release strategies,including intra-scleral delivery,episcleral delivery,as well as tans-scleral iontophoresis.Different devices and methods have their own advantages and disadvantages,for example,intrascleral delivery is somehow invasive,and episcleral delivery device needs to be made thin to prevent erosion of conjunctiva,iontophoresis needs to be frequently repeated as of its short-term effect.With the development of bio-material engineering technology,episcleral microfilm could become an ideal drug delivery route for posterior segment ocular diseases.

Between 40 to 60, especially after menopause, breast cancer have a higher incidence in women population. CA-153 ( carbohydrate antigen-153 ) is a tumor marker commonly used for monitoring breast cancer in clinic. However, CA-153 appears to have no clinical value for early diagnosis of breast cancer. Gene expression profiling technology becomes hot-spot through which some breast cancer related genes have been found. Moreover, the methods used for diagnosis of breast cancer also has certain shortcomings, and it is still uncertain that breast cancer is likely to be found at the early stage or be predicted in advance.

There have been problems in the existing multiple physiological parameter real-time monitoring system, such as insufficient server capacity for physiological data storage and analysis so that data consistency can not be guaranteed, poor performance in real-time, and other issues caused by the growing scale of data. We therefore pro posed a new solution which was with multiple physiological parameters and could calculate clustered background data storage and processing based on cloud computing. Through our studies, a batch processing for longitudinal analysis of patients' historical data was introduced. The process included the resource virtualization of IaaS layer for cloud platform, the construction of real-time computing platform of PaaS layer, the reception and analysis of data stream of SaaS layer, and the bottleneck problem of multi-parameter data transmission, etc. The results were to achieve in real-time physiological information transmission, storage and analysis of a large amount of data. The simulation test results showed that the remote multiple physiological parameter monitoring system based on cloud platform had obvious advantages in processing time and load balancing over the traditional server model. This architecture solved the problems including long turnaround time, poor performance of real-time analysis, lack of extensibility and other issues, which exist in the traditional remote medical services. Technical support was provided in order to facilitate a "wearable wireless sensor plus mobile wireless transmission plus cloud computing service" mode moving towards home health monitoring for multiple physiological parameter wireless monitoring.
Computer Systems ; Computers ; Humans ; Information Storage and Retrieval ; methods ; Internet ; Monitoring, Physiologic ; methods ; Software

Objective To investigate the clinical efficacy of combined acupuncture and medicine in treating kidney deficiency and phlegm dampness syndrome in obese polycystic ovary syndrome. Method Sixty-four patients were randomly allocated to two groups. The treatment group received combined acupuncture and medicine and the control group, oral administration of metformin. The course of treatment was three months. Pre-treatment and post-treatment indicators were statistically analyzed in the two groups of patients. Result BMI, waist to hip ratio, fasting insulin, fasting blood glucose and HOMA-IR improved significantly in the treatment group of patients after treatment; there were statistically significant pre-/post-treatment differences (P<0.05). Post-treatment BMI and waist to hip ratio improved more markedly in the treatment group than in the control group (P<0.05). Conclusion Combined acupuncture and medicine has a better therapeutic effect on kidney deficiency and phlegm dampness syndrome in obese polycystic ovary syndrome.

Transgenic animals are those in which recombinant DNA technology is used to remove a gene or a specific part of DNA with exogenous DNA, which is hereditable and can be expressed stably. Transgenic rodent technology, first demonstrated by Gordon in 1980,opened the door to the next step in biotechnology and gene regulation, which has been used to reproduce or mimic a part of a human disease in a complex cellular environment by creating in vivo models with germ line transmission of the introduced genetic regulatory elements. Animal models for drug discovery are developing increasingly, which is promoting the research and development new drugs greatly. The use and development of transgenic animals in pharmacological research is reviewed in this article.

Objective To observe the effect of retrograde sinus coronary perfusion on the function of myocardial cell membrane following retrograde sinus coronary perfusion Methods Thirty two isolated rabbit hearts were randomized into 4 groups: Group A, hearts were kept beating by retrograde perfusion with normothermic oxygenated blood; Group B, hearts received retrograde coronary sinus perfusion with warm blood cardioplegia; Group C, hearts received retrograde coronary sinus perfusion with cold blood cardioplegia; Group D, hearts received retrograde coronary sinus perfusion with cold crystalloid cardioplegia After 60 min retrograde perfusion, the hearts were switched on Langendorff Neely antegrade perfused working heart model for other 60 min Free calcium in myocardial cell, myocardial membrane MDA, ATPase and myocardial cell membrane fluidity were measured Results After retrograde coronary sinus perfusion, activity of ATPase and fluidity of the myocardial cell membrane in group A were higher than those in groups B, C and D The contents of myocardial cell membrane MDA and calcium in myocardial cell following retrograde perfusion were much lower in group A than those in groups B and C There was no remarkable difference between group C and group D Conclusion Retrograde perfusion of beating heart is superior to those perfused with warm blood cardioplegia in reducing free radical production, ameliorating paradoxical calcium accumulation in myocardial cell and maintaining activity of ATPase and fluidity of the myocardial cell membrane Warm blood cardioplegia is superior to cold blood cardioplegia

Objective To investigate the lonely behavior characteristics and related factors of chronic obstruetive pulmonary disease(COPD)patients.Methods 99 eases with stable patients with COPD and 90 healthy subjects were surveyed by SDS,ESLI.Relevant factors were analyzed.Results Patients with COPD showed difierent degrees of emotional isolation[(9.90±3.97),(6.15±5.22),F=82.539,P＜0.01)]and social intercourse isolation[(8.00±3.53),(4.31±2.46),F=73.540,P＜0.01)].Emotional isolation was mainly.Moderate emotional isolation and general social intercourse isolation were predominant,and emotional(r=0.399,t=3.476,P＜0.01)and social intercourse isolation(r=0.372,t=3.154,P＜0.01)were related to depression.Conclusion Patients with COPD presented obvious emotion and social intercourse isolation behavior.

Valproate (VPA) has long been used for the treatment of bipolar mood disorder. VPA is effective in control of mania and depression. Recent studies have demonstrated that VPA has profound neuroprotective effects in against various apoptotic stimuli. Moreover, VPA can promote neurogenesis, neuronal proliferation and differentiation. Although intensive research has been dedicated to VPA′s neuroprotection, the molecular mechanisms by which VPA protects neurons are still not fully understood. In this paper, recent progresses in the study of VPA′s neuroprotection and underlying mechanism are reviewed.

Objective:To study the antitumor effect of combination therapy with adenoviral transfer of mouse endostatin gene and cyclophosphamide chemotherapy on mouse Lewis lung cancer.Methods:Mouse endostatin gene was cloned into repli-cation-defective adenovirus by virus recombination technology to construct Ad-mES,adenovirus vector coding mouse endo-statin gene,and then its biological activities were surveyed in vivo.Animal models of mouse Lewis lung carcinoma in C57BL/6mice were established and randomly divided into4groups(n=10):including control,chemotherapy,gene therapy,and chemotherapy+gene therapy group.Mice received cyclophosphamide150mg/kg s.c.3times a day in a21-d cycle for chemotherapy;control group received0.9%normal saline100?l.Ad-mES(6?10 9 pfu/100?l)were administrated by multi-spots intra-tumor injection to every mouse in gene therapy group and combination therapy group.The tumor sizes were mea-sured at different times points.The expression of endostatin after transfer was detected by Western blot and the microvessel density(MVD)of tumor was measured by immunohistochemical examination.Results:Higher titers of recombinant aden-oviruses(Ad-mES)were constructed.Combination therapy with Ad-mES and cyclophosphamide inhibited tumor growth(P