Objective To describe the effect of four-handed technique on the prevention of cross infection in department of stomatology. Methods Patients (300 cases) in department of stomatology were collected and randomized into the observation group (using four-handed technique) and the control group (not using four-handed technique) with 150 cases in each group from May 2003 to October 2004. One patient was served by one doctor and one nurse in the treatment process. Sterilization isolation system was strictly implemented in order to prevent cross infection. The control group received routine treatment. The incidence rate of infection was compared in the two groups. Results Ten patients (6.7%) were infected in the control group. Only 3 patients (2.0%) were infected in the observation group, which was lower than that of the control group (P<0.05). Conclusions Four-handed technique played an important role in the prevention of cross infection by blocking the iatrogenic infection propagation.

Tumor markers lay the foundation of early diagnosis for hepatocellular carcinoma (HCC).Besides alpha-fetoprotein, glypican 3 is confirmed to be a novel tumor marker for HCC with high specificity and sensitivity, especially at immediately early stage of small carcinoma. Glypican 3 has high value of clinical application and may bring new hope for precise diagnosis of HCC.

Objective To study the effecticacy of interferon Combined with vitAD,nystatin with lidocain in treatment of children with herpangina. Methods 128 cases with herpangina were divided into two groups. All of them inhaled interferon,68 cases in treatment group were treated with vitAD and nystatin with lidocain. 60 cases in control group were given mengtuoshi. The treating course was two to five days. Results The total curative rate of treatment group ( 100% ) was much higher than that of control group (85 % ) ( P ＜ 0. 01 ). Conclusion Interferon and vi tAD and nystatin with lidocain was safe and efficacious in treatment of children with herpangina.

AIM: To optimize and establish a stir-bake with saltwater technology on Rhizoma Alismatis by multiple indexes orthogonal test methods, providing technical index on standardization of stir-bake with saltwater on Rhizoma Alismatis. METHODS: The orthogonal test was employed to determine the effects of the three factors including the quantity of saltwater、temperature and time affiliated with three levels Alisol B23-acetate, alcohol extractive and properties. RESULTS: Temperature and time had notable effects on experimental results, but the quantity of saltwater had no effect on the results, the temperautre was at (100 ?C), time was 10 minutes an adding 2 kg salt to 10 kg water per 100 kg drugs. CONCLUSION: The research is meaningful to standardization of stir-bake with saltwater on Rhizoma Alismatis.

Today's society, many people are plagued by tinnitus and deafness. Chinese medicine on tinnitus and deafness disease and its therapies and for-mulas have discussed quite wel . It is believed that according to the features of the organs, treating tinnitus and deafness from the five internal organs is much better .The virtual is mostly related to the heart, spleen and kidney, but the real mostly related to the heart, liver and lungs. In the treatment of tin-nitus and deafness from the perspective of the kidney, attention should be paid to nourishing the kidneys and strengthening the essence. Treating from the spleen, attaches great importance to the spleen and replenishing qi, clearing material. Treatment from the aspects of liver, the first is to make qi run unob-structed, to prevent generating phlegm and fire. From pulmonary vessels, great importance should be attached to the recovery of dispersing the descending lung. Treated from heart, benefiting qi and nourishing blood should be given much attention not only to make up for its deficiency, but also attach great importance to the qi, send a fire on its reduction. Sick of longer time, compatibility on the basis of syndrome differentiation and treatment to invigorate the flux pulse, aromatic resuscitation of Chinese herbal medicine shal be used.

TWo cases of systemic lupus erythernatosus with complement C_4 deficiency which were certified by xperiment were reported in this paper.The results of long follow-uP observation suggested thattwo patients ersistently exist lower complement C_4 in serum.One of these two patients havingcombined deficiency of C_3 and _ uffered from pulmonary infection repeatedly.Forthmore,webriefly analysed the relationship between complements nd ystemic lupus erythematosus.

Objective To investigate the influence of the glycemic control level of gestational diabetes melli-tus (GDM)to neonatal immunity system function.Methods 41 newborns whose mothers suffered from GDM and treated,were selected as the observation group.Inside the observation group,based on the glycemic control level,they were divided into 25 cases in higher satisfaction group and 16 cases in lower satisfaction group.20 cases from normal full -term and single -birth pregnancy were selected as the control group.The levels of serum immunoglobulin and the complement were observed in three different groups.Results The level of serum immunoglobulin(IgG)in lower satisfaction group was (5.2 ±1.4)g/L,which was significantly lower than higher satisfaction group (10.4 ±2.2)g/L and control group (10.3 ±1.9)g/L(t =8.957,8.470,P <0.01).The C3 and C4 levels in the higher satisfaction group [C3 (59.3 ±12.9)g/L,C4(39.4 ±11.2)g/L]and control group[C3 (59.9 ±13.8)g/L,C4 (39.8 ± 10.6)g/L]were significantly higher than the lower satisfaction group [C3(40.6 ±10.5)g/L,C4 (21.9 ±6.3)g/L] (t =4.612,4.854,5.956,5.685,all P <0.01 ).Conclusion Higher level of glycemic control on GDM mothers benefits neonatal serum immunoglobulin and the complement,and has positive impact on the immunity system and liver function.

Objective To analyze the influence of labor delivery ball exercise and free position for nursing on labor and birth outcome.Methods 110 cases of primipara were selected as the research subjects in our hospital, they were randomly divided into observation group and control group accorrded to digital method,55 cases in each group.The observation group received labor delivery ball exercise and free position for nursing,the control group received conventional way for nursing.The labor time of 1,2,3 stage,the total time of labor and 2h postpartum blood loss in two groups were compared,the VAS,VRS,PPI,VAS -A and LAS scores,the vaginal natural births rate of two groups were compared.Results The pain score,anxiety score in the observation group were (6.14 ±1.66)points and (3.63 ±1.24)points respectively,which were significantly lower than (8.46 ±1.27)points and (6.32 ± 1.33)points in the control group,and birth control score (171.21 ±19.75)points was also significantly higher than the control group (125.71 ±17.42)points,the differences between the two groups were statistically significant(t =6.115,3.938,5.327,all P <0.05 ).The first labor time and the total labor time in the observation group were (452.44 ±137.75)min and (493.02 ±137.99)min respectively,which were also significantly lower than the control group [(525.51 ±128.19)min,(576.41 ±132.13)min],the differences between two groups were statistically sig-nificant(t =3.097,5.406,all P <0.05).The natural childbirth success rate of observation group was 92.73%,which was obviously higher than 67.27% in the control group,the difference between the two groups was significant (χ2 =16.135,P <0.05).Conclusion Free position midwifery match with ball movement launched applied in natural birth can obtain good results,and has small injury,can improve the pregnant women's sense of control,reduce pain,it is worthy of clinical popularization and application.

Objective To study the effect of dehydroepiandrosterone (DHEA) on the immune system by measuring the apoptosis and expression of Fas/FasL mRNA of thymocytes. Methods The cell viability of thymocytes was determined by MTT assay. Thymocyte apoptosis was detected by agrese gel electrophoresis. The expression of Fas and Fas-L was analyzed by a semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) method. Results DHEA decreased the cell viability of thymocytes and induced thymocytes apoptosis and increased the levels of mRNA of Fas/FasL. Conclusion DHEA can induce apoptosis of thymocytes through Fas/Fas-L pathway. It suggests that DHEA may play a role in certain autoimmune diseases related to apoptosis disturbance.

Although single thermotherapy has certain curative effect for cancer treatment,it normally leads to the recurrence of tumor in clinical application.Multi-thermotherapy could be an effective approach to solve the problem.Multi-thermotherapy not only kills the tumor cells by heating cells above the physiological temperature,but also induces the anti-tumor immunity of the body to further kill the tumor cells.In order to obrain optimal treatment effect,the time interval of thermotherapy is often set once or twice a week for the consideration of thermotolerance.