2.Studies on chemical constituents of Epimedium koreanum
Chinese Traditional and Herbal Drugs 1994;0(11):-
Object In order to control the quality of crude drug, the total flavonoids of Epimedium koreanum Nakai were investigated Methods By repeated chromatographic methods 13 compounds were isolated and purified, and their structures were elucidated by physicochemical properties and spectroscopic methods (UV, IR, 1HNMR, 13 CNMR, HMBC, EI MS) Results They were identified as icariside II (Ⅰ), icariside I (Ⅱ), epimedoside C (Ⅲ), epimedin B (Ⅳ), icariin (Ⅴ), hyperoside (Ⅵ), hexandraside E (Ⅶ), anhydroicaritin (Ⅷ), maltol (Ⅸ), demethylanhydroicaritin (Ⅹ), insistol (Ⅺ), epimedoicarisoside A ( ⅩⅡ ) and (+) cycloolivil ( ⅩⅢ ) Conclusion Compounds Ⅶ, Ⅺ, ⅩⅢ were isolated from E koreanum for the first time, and Ⅶ was obtained from the plants of Epimedium L for the first time
3.CHEMICAL CONSTITUENTS OF PEUCEDANUM DECURSIVUM
Acta Pharmaceutica Sinica 2001;36(5):351-355
AIM To study the chemical constituents of Peucedanum decursivum. METHODS Various column chromatographies with silica gel and HPLC were employed for the isolation and purification. The structures of the compounds were elucidated on basis of spectral analyses and chemical methods. RESULTS Seven compounds were isolated from the roots of Peucedanum decursivum (Miq.) Maxim. 1 is 3′(S)-hydroxy-4′(R)-angeloyloxy-3′,4′-dihydroxanthyletin, named Decursitin D; 2 is 3′(S)-acetoxy-4′(R)-hydroxy-3′,4′-dihydroxanthyletin, named decursitin F; the others are 3′(S)-acetoxy-4′(R)-angeloyloxy-3′,4′-dihydroxanthyletin (3), Pd-C-IV (4), Pd-C-II (5), (+)-3′S-decursinol (6) and (+)-trans-decursidinol (7). CONCLUSION Compounds 1 and 2 are new compounds, while compounds 6 and 7 were isolated from Peucedanum decursivum (Miq.) Maxim for the first time.
4.Studies on the Active Constituents and Their Contents of stem and Leaf of Qianhu by RP-HPLC
Chinese Traditional and Herbal Drugs 1994;0(01):-
Chemical constituents of the aerial parts of Qianhu were compared with those present in the roots by RP-HPLC. It was found that the constituents in aerial parts of Peucedanum praeruptorum are similar and higher in content than that in the roots. So it is possible that the aerial parts can be used instead of the roots of the plant. But the chemical constituents in the aerial parts and the roots of P. decursivum are quite different, rendering it impossible to use the aerial parts instead of the roots of the plant.
5.Significance of changes in hepatic histology from patients with HBeAg(+)or HBeAg(-)chronic hepatitis B and normal or mildly elevated alanine aminotransferase
Lin LAN ; Yin KONG ; Lingyi ZHANG
Journal of Clinical Hepatology 2015;31(4):510-513
Alanine aminotransferase (ALT)abnormality is an indicator of the degree of liver inflammation caused by immune activation in patients with chronic hepatitis B (CHB).However,approximately half of the CHB patients with normal or mildly elevated ALT levels have concealed significant changes in hepatic histology.CHB patients with normal or mildly elevated ALT levels may have significant histopatho-logical changes in hepatic tissues,and those changes vary between HBeAg(+)and HBeAg(-)CHB patients.Attention and investigation on the clinical management of CHB patients with normal or mildly elevated ALT levels may have great significance in grasping the right treat-ment opportunity and reducing the risk of liver cirrhosis.
6.Triterpenoid Saponins from Roots of Gypsophila pacifica
Wei NIE ; Jianguang LUO ; Lingyi KONG
Chinese Herbal Medicines 2010;02(2):145-147
Objective To study the chemical constituents from the roots of Gypsophila pacifica. Methods The chemical constituents were isolated by various column chromatographic methods and their structures were identified by spectral data together with physicochemical analysis. Results Five compounds were isolated and identified as 3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl gypsogenin 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside (1), 3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside (2), 3-O-β-D-galactopyranosyl-( 1→ 2 )-[β-D-xylopyranosyl-( 1→ 3 ) ]-β- D-glucuronopyranosyl gypsogenin 28-O-β-D-glucopyranosyl-( 1→ 3 )-[β-D-xylopyranosyl-( 1→4 ) ]-α-L-rhamnop yranosyl-( 1 → 2 )-β-D-fucopyranoside (3),3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-β-D-glucopyranosyl-( 1→ 3 )-[β-D-xylopyranosyl-( 1→4 ) ]-α-L-rhamnopyranosyl-( 1→2 )-β-D-fucopyranoside ( 4), 3-O-β-D-galactopyranosyl-(1→2)-[β-D-xylopyranosyl-(1→3)]-β-D-glucuronopyranosyl quillaic acid 28-O-α-L-arabinopyranosyl-(1→4)-α-L-arabinopyranosyl-(1→3)-β-D-xylopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→2)-β-D-fucopyranoside (5). Conclusion The five compounds are isolated from this plant for the first time.
8.Chemical constituents in leaves of Euphorbia soongarica
Xinhong SHI ; Deran XU ; Lingyi KONG
Chinese Traditional and Herbal Drugs 1994;0(05):-
Objective To investigate chemical constituents in the leaves of Euphorbia soongarica(Xinjiang origin).Methods The leaves were extracted with 95% EtOH and partitioned with petroleum ether,CHCl3,and n-BuOH,respectively.Compounds were isolated by using repeated silical gel column chromatography and Sephadex LH-20 column chromatography.Results Eight compounds were identified on the basis of physiochemical properties and spectral data as chrysoeriol(Ⅰ),phthalic acid,bis-(2,5-dimethylhexyl) ester(Ⅱ),fraxetin(Ⅲ),3-(4-O-?-D-glucopyranosyloxy-3,5-dimethoxy)-phenyl-2E-propenol(Ⅳ),fraxin(Ⅴ),3-methylellagic acid-3′-O-?-D-xylopyranoside(Ⅵ),3,3′-dimethylellagic acid-4-O-sulphate(Ⅶ),and(+)-isolariciresinol-9-O-?-D-xylopyranoside(Ⅷ).Conclusion Compound Ⅶ is a new natural product,the other seven compounds are obtained from this plant for the first time.
10.Chromones from Angelica morri Hayata
Shi SUN ; Lingling XU ; Lingyi KONG ; Hanqing ZHANG ; Shanan HE
Journal of China Pharmaceutical University 2003;(2):125-127
AIM:Bioactive constituents were expected to be obtained from the roots of Angelica morri Hayata. METHOD:They were extracted with 95% alcohol and isolated by using column chromatography and recrystallization methods. The structures were elucidated by means of physico-chemical data and UV,IR,1HNMR, 13CNMR,and EIMS. The inhibitory effect on the constriction of rat aortic rings was induced by K+ or Ca2+. RESULT:3′S-(-)-hamaudol,3′S-(-)-Ο-acetylhamaudol,3′R-(+)-hamaudol,and (±)-hamaudol were isolated from the pieces of Radix Angelica Morri. The inhibitory rate of 3′S-(-)-Ο-acetylhamaudol and (±)-hamaudol on the above pharmacologic model appears the relation of quantity response. CONCLUSION:All the above compounds were found in this species for the first time,and(±)-hamaudol is a new. One of effect mechanisms of 3′S-(-)-Ο-acetylhamaudol and (±)-hamaudol diluted aorta could contribute to be inhibiting Ca2+ influx of vascular smooth muscle.