1.DNA Extraction Method Research for DNA Bar Code Analysis of Chinese Medicinal Materials
Zhonggang DUAN ; Qionglin HUANG ; Jinfen YANG ; Lingwu DIAO ; Ruoting ZHAN ; Rui HE ; Hui XU ; Ping YAN ; Weiwen CHEN
Traditional Chinese Drug Research & Clinical Pharmacology 2000;0(05):-
Objective To establish a DNA extraction method for DNA barcoding analysis of Chinese medicinal materials.Methods Seven different DNA extraction methods were used to extract DNA from 6 medicinal recalcitrant plants which are rich in secondary metabolites.Results CTAB method 3 was fast,simple,universal and effective,by which a high DNA concentration and qualified ratio were obtained as compared with the other methods.The DNA extracted by this method could provide good results for DNA barcoding analysis.The main improved steps of this methods were as follows:①adoption of 3 %CTAB rather than 2 %CTAB in the exaction;②adding 1 %polyvinylpyrrolidone(PVP) and 0.2 %?-mercaptoethnoal in extraction solution to remove secondary metabolites and to prevent DNA degradation;③centrifuge at 10000 r/min for 15 min to remove protein and impurity.Conclusion CTAB method 3 is a proper method of DNA extraction for DNA barcoding analysis of Chinese medicinal materials.
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