[Objective]To investigate the feasibility of the dermal pluripotent stem cells in the repairing of cartilage defects.It was aimed to provide experimental basis in view of cartilage defects of expanding the repair of cartilage defects seed cells selection. [Method]Neonatal cyanotic blue rabbits were used.Dermal tissue directly isolated through mechanical method and cells through enzymatic digestion were obtained.The growth characteristics of adherent adhesion of stem cells were used to obtain high cloned cells,which were subcultured.The cell concentration of 1?106/ml was cultured with polylactic acid scaffold for one weeks,and the obtained result was implanted into full-thickness articular cartilage defects of rabbits.Thirty cyanotic blue rabbit(60 joints)for about 3~5 months were randomly divided into three groups:dermal pluripotent stem cell / scaffold polylactic acid in group A,polylactic acid scaffold in group B,controls in group C.Twenty joints for each group.The rabbits were killed by air embolization at 12 weeks,restoration organization was extracted and stained by HE and toluidine blue.According to the repairing result of cartilage defect,gross and histologic scoring was made,and analyzed by statistics.The comparison of score difference between each groups was performed statistically.[Result]Gross and histological observation demonstrated that in group A organizations had smooth surface,appeared transparent,good integration with surrounding cartilage and subchondral bone.In group B there was a tiny transparent cartilage,and the fiber cartilage repair accounted for much proportion.The surface of group C showed some defects,mainly characterized by fibrous cartilage repair.After gross and histological observations statistics score analysis was performed.Results showed that group A is the most optimal(P

Objective To study the preparation method of compound tissue-engineering scaffolds of the PLA/silk fibroin and evaluate its biological features.Methods The PLA scaffolds matrix were dipped into the silk fibroin solution,then dried,and PLA/silk fibroin scaffolds were prepared.There were two groups in the experiment,one group was PLA group,and the other one was compound scaffolds group.According to ISO-10993 standard,hematolysis test,dynamic coagulation time test,cell toxicity test,stimulation test and pyrogen test were performed in two groups,and the results were compared betwen two groups.Results In the stimulation test,the two kinds of materials had equally not aroused the obvious animal skin stimulation,it showed that the experiment was in accordance with the standard.In the pyrogen test,the two scaffolds material aroused the animal temperature rising without exception under 0.2℃ and the total number of degree was under 1.0℃,therefore there was no obvious difference between two groups.In the hematolysis test,the hemolysis rates of the two scaffolds samples were smaller than 5% equally(P=0.000),which indicated that the hemolysis of the compound scaffolds was better than that of the PLA scaffolds.In the dynamic coagulation time test,the coagulation time of the compound scaffolds(37 min) was longer than that of the PLA scaffolds(26 min).The anti-coagulation ability of the compound scaffolds was better than that of the PLA scaffdds.In the cell toxicity test,the cell growth situation of the compound scaffolds group was obviously better than that of the PLA group,and at the meantime the cell toxicity of the compound scaffolds was obviously smaller than that of the PLA scaffolds.Conclusion The material of PLA/silk fibroin compound scaffolds has the advanced biological consistent compared with the simplex scaffolds.Accordingly,the PLA/silk fibroin can be used as a scaffolds matrix to be transplanted into the body.

Objective To determine the relationship between the level of plasma homocysteine and coronary calcification in patients with different blood glucose levels. Methods By measuring plasma homocysteine and coronary calcification in 30 cases of diagnosed diabetes (T2D) ,29 cases of diagnosed impaired glucose tolerance (IGT) in patients and 27 cases with normal, we compared the level of plasma homocysteine and coronary calcification in patients with different blood glucose levels. Results We found significant diffieronces among three groups of the level of plasms homocysteine and coronary calcification (P ＜ 0.01). The plasma homocysteine levels were(19.31 ±3.17) μmol/L, (13.85 ± 1.62) μmol/L, (9.80 ± 1.78) μmol/L in the T2D,IGT and normal groups,respectively. The coronary calcification scores were 207.80 ± 154.10,63.24 ± 10.46,14.47 ± 5.16 in the T2D, IGT and normal groups, respectively. The plasma homocysteine level and coronary calcification score increased with the glycosylated hemoglobin rise in the normal,IGT and T2D groups((4.51 ±0.48)%, (6.13 ±0.31)% and (7.69 ±0.81)%, respectively). Conclusions The plasma homocysteine level is a strong independent predictor of type 2 diabetes and also an important factor of coronary artery event occurrence and develepment.

A novel genomic island (GI) in Streptococcus suis serotype 2(SS2) was identified, which resided in the highly virulent strains but not in the hypo-virulent strains or avirulent strains of SS2 of the Chinese isolates. This newly discovered GI strain was designated as SSGI4 and its whole length of genome was 11 269 bps, sharing the typical properties of pathogenicity islands, such as the distinct G+C content, a mosaic architecture characteristics and the specificity for virulent isolates. There were 11 genes within SSGI4, in which some genes were putative cell surface protein genes and others were amino acid-binding protein genes. Our finding sheds light on the investigation of horizontal gene transfer in SS2 and their influence on pathogenicity.

Objective:To explore the key targets and mechanism of Bielong Ruangan decoction in the treatment of liver cancer based on network pharmacology and molecular docking.Methods:Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) database, PubChem database and PharmMapper database were used to search and screen the chemical components and related targets of Bielong Ruangan decoction and the targets of liver cancer diseases. The network diagram of " Bielong Ruangan decoction-traditional Chinese medicine-active ingredient-predicted target-disease" was constructed; Protein-protein interaction (PPI) network were analyzed through String database; gene ontology (GO) enrichment analysis was performed through WebGestalt database; Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis was carried out through KEGG Orthology Based Annotation System (KOBAS) database; Molecular docking of the active components and core target proteins of Bielong Ruangan decoction was carried out by using PyMOL, Auto DockVina and other software.Results:Bielong Ruangan decoction had 67 active components, 154 liver cancer targets and 244 pathways. According to the analysis of network pharmacology, Bielong Ruangan decoction may play an anti-cancer role through key targets such as epidermal growth factor receptor (EGFR), mitogen activated protein kinase 1 (MAPK1), estrogen receptor 1 (ESR1), MAPK8, serine threonine protein kinase 1 (AKT1), MAPK14, cysteine protease 3 (CASP3), cyclin-dependent kinase 2 (CDK2), bone morphogenetic protein 2 (BMP2), aldose reductase (AKR1B1) and other key targets. KEGG enrichment analysis showed that the treatment of liver cancer by Bielong Ruangan decoction involved the regulation of vascular endothelial growth factor (VEGF) signaling pathway, tumor necrosis factor (TNF) signaling pathway, thyroid hormone signaling pathway, T cell receptor signaling pathway and other pathways. The results of molecular docking showed that the binding energy of all compounds to protein was less than -5.6 kcal/mol, indicating that each compound and each protein could bind well.Conclusions:Bielong Ruangan decoction participates in the treatment of liver cancer through " multi-component, multi-target and multi-channel" ways, and plays an anti-cancer role mainly by regulating the proliferation and invasion of tumor cells and tumor inflammatory microenvironment.

Objective To explore dynamic characteristics of the gait for the elderly with different fall risks before and after obstacle crossing. Methods Twenty-seven elderly people in community were graded as fall risk by using the time up and go test and five-time sit to stand test. The plantar pressure parameters of the elderly before and after obstacle crossing were measured and analyzed by foot pressure measurement system. Results There was no significant difference in the characteristic value of bimodal curve of overall plantar pressure between the high and low fall risk groups before and after obstacle crossing(P>0.05). The center of pressure (COP) trajectory in X direction of high fall risk group after obstacle crossing was significantly greater than that of low fall risk group (P<0.05). Before obstacle crossing, the peak pressure of the 3rd metatarsal of supporting foot was higher in high fall risk group than that in low fall risk group (P<0.05). After obstacle crossing, the peak pressure of the 1st phalanx of supporting foot was significantly lower than that in high fall risk group (P<0.05), while the lateral heel impulse in high fall risk group was significantly larger than that in low fall risk group (P<0.05).The distribution patterns of contact area of the foot for the elderly in two groups before and after obstacle crossing were basically the same, and there was no significant difference in contact area of each plantar region (P>0.05). Conclusions The support time of the elderly with high fall risk is longer than that of the elderly with low fall risk during obstacle crossing, the peak pressure of plantar metatarsal region of the crossing leg increases, and the plantar COP curve shows asymmetry, with an increase in transverse range of the coronal plane. In clinical evaluation, plantar pressure characteristics of people with fall risks during obstacle crossing should be focused on.