ObjectiveTo design and construct CRISPR/Cas9 gene editing system targeting Tsc1 and Tsc2 genes,and verify the effectiveness of gene editing at cellular level.MethodsThree sgRNA guide sequences were designed for mouse Tsc1 and Tsc2 genes respectively. The sgRNA expression vector was constructed and co-transfected with the Cas9 expression plasmid into mouse N2a cells. After the positive cells were obtained through drug screening,the DNA fragments at the targeting site were amplified by PCR,and the targeting efficiency was verified by TA clone sequencing.ResultsThe five targets of Tsc1-M-sgRNA2 and Tsc1-M-sgRNA3 of Tsc1 gene and Tsc2-M-sgRNA1,Tsc2-M-sgRNA2 and Tsc2-M-sgRNA3 of Tsc2 gene were all edited,and the editing efficiency was 40%,80%,30%,30% and 20%,respectively.ConclusionA CRISPR-Cas9 gene editing system with editing efficiency targeting mouse Tsc1 and Tsc2 genes was successfully constructed.

Objective: To evaluate the effect of postoperative chemotherapy on bone density change in the patients with breast cancer and the therapeutic effect of 99 TC-MDP.Methods: Totally 58 breast cancer patients with postoperative chemotherapy were se-lected, and the changes of bone density , blood calcium element (BGP), alkaline phosphatase (ALP) and 24 h urinary calcium levels were observed .After the chemotherapy , the osteoporosis patients were divided into the control group and the observation group , and the control group was given calcium carbonate D 3, while the observation group was given calcium carbonate D 3 combined with 99 TC-MDP. The bone density , BGP, ALP and 24 h urinary calcium levels were compared between the two groups .Results:After the chemothera-py, the bone mineral density of lumbar vertebra and thighbone was decreased significantly , BGP, ALP and 24 h urine Ca were in-creased significantly, and compared with those before the treatment , the differences were statistically significant (P<0.05).After the 3-course postoperative chemotherapy , the incidence of osteoporosis was 22.4%(13/58), and after the six-course chemotherapy, the incidence of osteoporosis was 39.7%(23/58).The bone mineral density of lumbar vertebra and thighbone in the observation group was increased significantly , BGP, ALP and 24 h urine Ca were decreased significantly , and compared with those in the control group , the differences were statistically significant (P<0.05).Conclusion: Postoperative chemotherapy for breast cancer can lead to reduced bone density , and 99 TC-MDP in the treatment of osteoporosis induced by breast cancer postoperative chemotherapy is safe and effective .

Objective To investigate the efficacy of externally applied diclofenac diethylamine emulgel on the prevention of PICC-induced mechanic phlebitis. Methods One hundred cancer patients with PICC were divided into two groups. diclofenac diethylamine emulgel and mucopolysaccharide polysulfate cream were applied three times a day for 7 days after catheterization in the two groups,respectively. Results There was no significant difference on the incidence of mechanic phlebitis between the two groups (4% vs. 8%,P>0.05). Conclusion The efficacy of externally applied diclofenac diethylamine emulgel on the prevention of PICC-induced mechanic phlebitis is the same as mucopolysaccharide polysulfate cream. Except their common virtue of convenient and simple operation,the price of diclofenac diethylamine emulgel is lower than mucopolysaccharide polysulfate cream and worthy to be spread in clinical practice.

Objective To provide the scientific guidance for the prevention of lead poisoning in the children ,the lead concentra‐tions in the blood of children in Xiamen are investigated .Methods 11 271 cases of outpatients with the age of 0 -10 years old in maternity and child care hospital were surveyed randomly .The lead concentrations in the blood of these children were determined by graphite furnace atomic absorption spectrometer .Results From 2010 to 2013 ,the whole blood lead levels of 11 271 children was detected .The mean blood lead levels was 51 .0 μg/L .The boy lead poisoning rate was higher than girls in different age groups ,the difference was statistically significant (P<0 .05) .Both the above indices increased gradually with the increase of age in the range of 1-6 years old ,and the mean blood lead levels reached its peak in preschool age ,the mean blood lead did not increased in school‐age children ,and the lead poisoning rate showed the downward trend .Conclusion The lead poisoning rate of children in Xiamen is close to the average level of other cities ,however ,the blood lead level tends to increase with the increase of age .The effect of lead pollu‐tion on physical health of children shall be paid more attention to nowadays .

Objective To investigate the clinicopathological relation between the TNF-? and IgA nephropathy. Methods TNF-? levels in serum, urine and renal tissues of 50 patients with IgA nephropathy were measured, and 10 patients with minimal change nephrotic syndrome(MCNS) and 10 healthy subjects served as negative control group. Results The serum and urine levels of TNF-? in the patients with IgA nephropathy were significantly higher than those in the patients with MCNS and healthy subjects, and had a significant positive correlation with the degree of proteinuria and renal demage. TNF-? expression was mainly localized in the cytoplasm of the proximal renal tubular epithelial cells, and the interstitium with more monocyte-macrophages infiltration. Conclusion TNF-? took part in the onset of hematuria and proteinuria, was correlated with the degree of renal damage, and played an important role in the aggravation of IgA nephropathy.

Objective To investigate the value of neutrophil-to-lymphocyte ratio in evaluation of prognosis in patients with early cervical cancer before radical resection. Methods A restropective study was performed in 76 patients who underwent radical resection for early cervical cancer in our hospital between 2007 and 2009. All patients were diagnosed by pathology. All patients did not accept neoadjuvant therapy , the median value of neutrophit-to-lymphocyte ratio (NLR) was 1.94 (0.73-9.31 in range). Based on this value of 1.94 as threshold, all patients were divided into 2 groups: a low NLR(≤1.94, n=38) group and a high NLR ( >1.94, n=38) group. Univariate and multivariate analyses were performed to assess the effectiveness of preoperative NLR to the prognosis in patients who underwent radical hysterectomy combined with pelvic lymph node dissection. Results The preoperative NLR was different significantly in the depth of stromal infiltration, lymphatic metastas, FIGO stage and pathological type between the low NLR group and the high NLR group. The high NLR group, lymphatic metastas, postoperative radiotherapy and FIGO stageⅡ were all risk factors for prognosis and disease-free survival in univariate analysis.Multivariate analysis revealed that NLR, lymphatic metastas and FIGO stageⅡwere independent risk factors for disease-free survival. FIGO stageⅡand lymphatic metastasis were independent risk factors for overall survivaI. Conclusion Preoperative NLR was found to correlate to unfavorable histopathologic features of cervical cancer. The preoperative NLR may be used as a useful and easy biomarker for disease-free survival in patients with cervical cancer, but not an independent risk factor for poor prognosis.

The composition of moxibustion quantity is discussed, and it is believed that the effective moxibustion quantity is a certain threshold range, which is influenced by the functional state of the patient and different moxibustion methods. Based on the foundation of conventional moxibustion quantity, the quantity should be adjusted according to patient's constitution, age,moxibustion area, affected region, nature of disease and the sensitivity. Through different methods and techniques, the stimulus intensity and time can be altered to approach a proper moxibustion quantity.

Objective To investigate the relationships between exercise and exercise self-efficacy and knowledge in pregnant women. Methods A total of 274 pregnant women were recruited from a tertiary level first class hospital from July to November 2015 in Guangzhou by convenience sampling method. They were investigated with the social relational scale, the Pregnancy Physical Activity Questionaire, the Pregnancy Exercise Self-efficacy Scale, the Pregnancy Exercise knowledge Scale. Results Only 22.6％ (62/274) of the women met the international guideline for exercise. Score of the exercise self-efficacy and exercise were positively correlated(r=0.203, P=0.000). Score of the knowledge and exercise were positively correlated(r=0.103, P=0.032). Score of the exercise self-efficacy and knowledge were meaningless(r=0.098, P=0.106). Conclusions It is suggested to take more actions to enhance the recognition of pregnant women for exercise. Conviction formed after thinking knowledge gradually, by faith again dominate action, ultimately promote the perinatal health of pregnant women.

Objective To test the reliability and validity of the Chinese version of the Pregnancy Exercise Self-Efficacy Scale(P-ESES) among Chinese population. Methods The Chinese version of P-ESES was translated from the original one,back-translated and adjusted for cultural adaptation. The reliability and validity were tested among 274 eligible pregnant women recruited in one obstetric outpatient clinic from a tertiary hospital in Guangzhou using convenience sampling. Results The content validity index (CVI) of the Chinese version of P-ESES was 0.98. Factor analysis extracted three common factors,which explained 60.649% of the total variance,and each item had high fac-tor loading quantity(>0.4). The test-retest reliability was 0.531,and Cronbach's α coefficient was 0.804. Conclusion The Chinese version of the P-ESES has been proved to be reliable and valid. It is a valuable tool for evaluating exercise self-efficacy among pregnant women in mainland China.