Objective To explore and establish the possible method of low-intensity pulsed ultrasound(LIPUS) irradiation directly combined with SonoVue microbubbles could enhance 5-azacytine (Aza) induced cardiomyogenic differentiation of human bone marrow mesenchymal stem cellin(MSCs)vitro,initially explore the appropriate irradiation condition,and to provide experimental basic data for ultrasound-mediated microbubble destruction application in MSCs transplantation for acute myocardial infarction.Methods (1) In order to screen out the appropriate ultrasound irradiation intensity and time without conspicuous harm to MSCs,stem cells were exposed to different ultrasound intensity irradiation (0.11,0.33,0.55,0.77,0.99 W/cm2) and time (0,30,60,90 s) and then cell proliferation determined by methyl thiazolyl tetrazolium(MTT).In order to screen out the appropriate microbubble-to-cell ratio without conspicuous harm to MSCs,stem cells of different microbubble-to-cell ratios (0,10,50,100,150) were exposed above the screened ultrasound irradiation intensity and time and then cell proliferation determined by MTT.(2) MSCs were divided into four groups and induced cardiomyogenic differentiation by different inducing systems in vitro as follows:no-treatment group,5-Aza inducing group(10 μmol/L),microbubble and ultrasound group,5-Aza inducing (10 μmol/L) with microbubble and ultrasound group,followed by four weeks of further culture.The morphological changes were studied daily under phase-contrast microscope,then the expression of cardiac troponin-T (cTnT) was detected by immunocytochemistry method to analyze and compare differentiation rate of cardiomyocyte-like cells in each group.Results (1)The direct ultrasound irradiation condition of no conspicuous harm to MSCs,but with destruction was intensity of 0.55 W/cm2,exposure time of 30 s and microbubble-to-cell ratio of 50.(2) This study confirmed the feasibility of direct ultrasound exposure of LIPUS on MSCs in vitro.(3) At 28 days after induction,the stem cells of 5-Aza inducing group and 5-Aza inducing with microbubble and ultrasound group expressed cTnT and the positive rate of the latter was higher than the former by immunocytochemistry (13.69％ vs 34.13％,P ＜0.05).Conclusions The appropriate ultrasound direct irradiation condition was intensity of 0.55 W/cm2,exposure time of 30 s and microbubble-to-cell ratio of 50.Appropriate conditions of LIPUS could enhance 5-Aza induced cardiomyogenic differentiation of MSCs in vitro.

Objective To conduct the study and comparative analysis on the performance of foreign hepatitis D virus (HDV) an-tibody test kits .Methods The collected 1 000 serum samples were tested by 3 kinds of different reagent :test reagent ,reference rea-gent and third party reagent .The detection results were processed by the statistical method and the judgement was performed base on the S/Co value .Results The Kappa analysis showed that the Kappa value was 0 .950(P<0 .01) .The specificity was 100% ,the sensitivity was 97 .73% and the area under ROC was 0 .986 .Conclusion The test kit has a relatively high accuracy and high consis-tency compared with the diagnostic reagent kits now widely used in domestic .Increasing the clinical detection rate of HDV can play a helping role on prevention ,early diagnosis and treatment of the disease .

The current study suggested that gastrointestinal motility has a close relationship with ascending-descending theory of spleen and stomach of TCM.Gastrointestinal symptoms caused by Qi disorder of spleen are identical with the clinical manifestations of gastrointestinal motility disorders.Although spleen and stomach act as the pivot of Qi transporting in the body,the dysfunction of liver,lung,kidney,and heart may also cause disorders of Qi in ascending and descending,manifested as pathological gastrointestinal motility.Therefore,when gastrointestinal motility appears abnormality,we should pay attention to other organs besides analyzing dysfunction of spleen and stomach

The mechanism of long chain non-coding RNA (lncRNA) is diverse. LncRNA can regulate the expression of genes at multiple levels and is closely related to some pathological process, such as tumor formation, viral replication, inflammatory damage, etc. The abnormal expression of lncRNA is involved in the patho-physiological changes of the disease, and plays a key role in many links, such as the stability of mRNA, the regulation of translation level, protein transport, RNA processing and modification, and so on. The development of sepsis inflammatory injury is a complex process involving multiple genes and pathways, in which the abnormal structure and expression of many encoding genes or non-coding genes exist.This paper review the research progress on lncRNA in the regulation of chondriokinesis, pro-inflammatory cytokines, calcium ion transport, nuclear factor-κB (NF-κB) in signal pathway and other inflammatory lesions, which may provide a new method for the diagnosis and treatment of sepsis.

Objective To observe the variation of the levels of γδT cells,CD4+ and CD8+ T lymphocytes in patients with acute and subacute brucellosis before and after treatment.Methods A total of 159 patients with brucellosis were enrolled from the Second People's Hospital of Tianjin between September 2009 and August 2014,of which 122 were with acute phase and 37 with subacute stage.γδT cells,CD4+ and CD8+ T lymphocytes in peripheral blood of all patients before and after treatment were detected by flow cytometry.Thirty healthy controls were also enrolled.Measurement data were analyzed using t test and nonparametric Kruskal-Wallis test.Results The medians of γδT cells,CD4+ and CD8+ T lymphocyte counts of brucellosis patients with acute stage before treatment were 84.0/μL,780.5/μL and 774.0/μL,respectively,and those with subacute stage were 76.0/μL,887.0/μL and 890.0/μL,respectively.Those in control group were 49.0/μL,772.5/μL and 592.5/μL,respectively.γδT cells (U=114.5,P =0.001) and CD8+ T lymphocyte counts (U=1 185.5,P =0.003) in acute phase group were elevated than control group.γδT cells (U=359.0,P=0.013),CD4+ T lymphocyte counts (U=325.5,P=0.004) and CD8+ T lymphocyte counts (U=316.5,P=0.003) in subacute phase group were increased than control group.CD4+ T lymphocyte counts in subacute phase group were elevated than acute phase group (U=1 619.0,P=0.009).There were 31 patients with acute phase and 30 patients with subacute phase who completed 6-week treatment.γδT cells ([120.7 ± 104.9]/μL vs [82.2 ±85.3]/μL;t=3.568,P=0.001),CD4+ T lymphocyte ([802.1±300.6]/μL vs [663.0±280.6]/μL;t=2.904,P=0.007) and CD8+ T lymphocytes counts ([908.1±422.7]/μL vs [651.1±241.7]/μL;t=3.885,P=0.001) of brucellosis patients with acute stage before and after treatment were significantly different.γδT cells ([67.1± 38.4]/μL vs [48.4±32.1]/μL;t=2.695,P=0.012),CD4+ T lymphocytes ([817.1± 147.4]/μL vs [650.8± 196.5]/μL;t=5.301,P＜0.01) and CD8+ T lymphocytes counts ([703.7±191.5]/μL vs [537.4±186.0]/μL;t=5.152,P＜0.01) of brucellosis patients with subacute stage after treatment were significantly decreased compared with those of patients before treatment.Conclusions γδT cells,CD4+ and CD8+ T lymphocytes in patients with acute and subacute brucellosis are all in a state of immune activation.The levels of activation are highly correlated with disease progression.And detection of the variation of these cells contributes to understand the immune state of the acute and subacute brucellosis.

Objective To compare the features of adolescents' normal fear between urban and rural areas in China. Methods 648 Urban and 478 rural adolescents were investigated with the revised Fear Survey Schedule For Children -II . Results (1)The most common fear of adolescents in urban and rural areas was very same,namely taking dangerous drugs,someone in my family having an accident,not being able to breath,someone in my family dying,nuclear war,my parents separating or getting divorced,being kidnapped,but only one item was not same ,namely urban adolescents fear having no friends ,rural adolescents fear AIDS. (2)The amount of fear items( t =11.42,P <0.01) and the degree of general fear (urban =1.62±0.28,rural =1.83±0.26,t =12.72,P <0.01) ,each dimension fear(P <0.01) of rural adolescents were significantly higher than that of urban adolescents.(3)The degree of fear about danger and injure( F =85.46,P <0.01),animal (female=1.84±0.47,male=1.54±0.45,F =156.86,P <0.01),social relationship ( F =32.23,P <0.01)of female adolescents were significantly higher than that of male adolescents.(4) In all adolescents ,the degree of the general fears( F =13.781,P <0.01) danger and injure fears( F =17.164,,P <0.01) decreased gradually as they grew up ,and the degree of unknown and uncertainty fear(( F =5.01,P <0.01),social fear ( F =4.42,P <0.05)of middle school students were higher than that of high school students; urban middle school students were significantly higher than that of urban high school students in failure and punishment fear.Conclusion (1)There are significant consistency on the most normal fears,the trend of development about the general fears,danger and injure fears,unknown and uncertainty fear,social fear for adolescents between urban and rural areas .(2)The amount of fear items and the degree of general fear ,each dimension fear of school-aged rural adolescents were significantly higher than that of urban adolescents;and there isn't common trend of development on the failure and punishment fear for rural and urban adolescents.

Objective To study the effects of combinatory application of pressure-ulcer risk evaluation and nursing management system. Methods 65 483 children discharged during the whole year 2014 as control group used conventional pressure ulcer prevention and management, while 67 391 children discharged during the whole year 2015 as osbervation group used risk assessment and systematic management based on the control group. Braden-Q scale was recruited to evaluate the pressure-ulcer risk of inpatients. Compared between the observation and the control groups were the two key data:the high-risk prediction of pressure ulcer and the incidence of pressure-ulcer. Results While 56 of 544 high-risk children in the control group developed ulcer with an occurrence of 10.3%;only 30 of 747 such children in the observation group developed with an occurrence of 4.0%, and none died for complications. Significant difference existed between the two groups (χ2=3.629, P<0.05). Conclusions Introduction of pressure-ulcer risk evaluation tools and nursing management system, while providing risk evaluation of pressure ulcers, offers guarantee to timely reports, efficient care and treatment of the high-risk children, thus decreasing the occurrence of pressure ulcer.

Objective To study the effect of external application of Wuwei Shuangbai Powder on relieving primary liver cancer pain and to observe its effect on hemorheology indexes.Methods A double-blind,randomized and controlled trial was carried out in 60 primary hepatocellular carcinoma patients with cancer pain.The patients were divided into control group and treatment group,30 cases in each group.The treatment group was given external application of Wuwei Shuangbai Powder and oral use of placebo capsules,and the control group was given external application of placebo powder and oral use of celecoxib capsules.The treatment for the two groups lasted for 7 days.Before and after treatment,pain was scored by Numeric Rating Scale (NRS),and the peripheral blood hemorheology indexes of both groups were observed.Results (1) After treatment,the NRS pain scores of both groups were decreased compared with those before treatment (P ＜ 0.05 or P ＜ 0.01),and the scores of the treatment group were significantly lower than those of the control group (P ＜ 0.05),indicating that the treatment group had better effect on relieving cancer pain.(2) After treatment,the indexes of hemorheology of treatment group were significantly higher than those of the control group,and the differences of the indexes except the hematocrit were significant between the two groups(P ＜ 0.05 or P ＜ 0.01).Conclusion Wuwei Shuangbai Powder is effective for the treatment of primary liver cancer pain.The mechanism may be related to the improvement of hemorheology of liver cancer patients.

Objective To investigate the association betweent clinical markers of N‐terminal probrain natriuretie peptide (NT‐proBNP) ,cardiac troponin I (cTnI) and C‐reaction protein (CRP) in the patients with acute respiratory distress syndrome (ARDS) caused by severe pneumonia and to reveal the pathophysiologic process of myocardial injury after ARDS .Methods Totally 33 patients diagnosed as ARDS caused by severe pneumonia were divided into 3 groups:mild group ,moderate group and severe group according to the New Berlin definition of ARDS .Arterial blood gas analysis ,NT‐proBNP ,cTnI and CRP were observed be‐fore the treatment .Statistics were used for the analyses .Results The NT‐proBNP level in the severe group was significantly higher than that in the mild and moderate groups (P<0 .01);the NT‐proBNP level in the moderate group was also higher than that in the mild group (P< 0 .05) .Besides ,the cTnI level in the severe group was significantly higher than that in the mild and moderate groups (P<0 .01);however ,the cTnI level had no statistical difference between mild and moderate groups (P> 0 .05) .The CRP level in the severe group was significantly higher than that in the mild (P<0 .05) .Moreover ,the NT‐proBNP level of 33 patients was positively associated with the both CRP and cTnI level (P<0 .05) .Conclusion The seriously inflammatory response mediates myocardial injury probably because of ARDS .Combined NT‐proBNP and cTnI testing is important for evaluating the condition of ARDS at the early stage .

Objective To provide the scientific guidance for the prevention of lead poisoning in the children ,the lead concentra‐tions in the blood of children in Xiamen are investigated .Methods 11 271 cases of outpatients with the age of 0 -10 years old in maternity and child care hospital were surveyed randomly .The lead concentrations in the blood of these children were determined by graphite furnace atomic absorption spectrometer .Results From 2010 to 2013 ,the whole blood lead levels of 11 271 children was detected .The mean blood lead levels was 51 .0 μg/L .The boy lead poisoning rate was higher than girls in different age groups ,the difference was statistically significant (P<0 .05) .Both the above indices increased gradually with the increase of age in the range of 1-6 years old ,and the mean blood lead levels reached its peak in preschool age ,the mean blood lead did not increased in school‐age children ,and the lead poisoning rate showed the downward trend .Conclusion The lead poisoning rate of children in Xiamen is close to the average level of other cities ,however ,the blood lead level tends to increase with the increase of age .The effect of lead pollu‐tion on physical health of children shall be paid more attention to nowadays .