OBJECTIVE:To investigate the protective effects of Yiweikang(YWK)capsule on gastric mucosa of the model rats with gastritis and gastric ulcer.METHODS:The experimental rats were randomly divided into five groups:YWK high-dosage group,YWK moderate-dosage group,YWK low-dosage group,positive control group(treated with drenched cime?tidine capsule,0.1g/kg),and negative control group(provided with drenched isovolumic distilled water or sodium chloride)in order to observe the effects of YWK for excitable gastric ulcer in mice and gastric ulcer in rats induced by alcohol and pylorus-ligature.RESULTS:Compared with the negative controls,gastric acid,gastric juice and ulcer indices in YWK group declined,most significantly in high-dosage group.CONCLUSION:YWK in oral administration has preventive effects for excitable ulc_ er,alcohol induced ulcer and pylorus-ligature induced ulcer.It also can help the recovery of gastric mucosa of ulcer model rats.

OBJECTIVE:To observe the clinical efficacy of acupoint application of antiemetic cream in the treatment of che-motherapy-induced nausea and vomiting. METHODS:90 cases of non-small cell lung cancer(NSCLC)(stage Ⅱ to Ⅲ)treated by chemotherapy(taxol+cisplatin)were randomly divided into antiemetic cream group,Weifuan group and control group,with 30 cas-es in each groupby drawing lots. Control group was given intravenous injection of ondansetron hydrochloride 8 mg 30 min before chemotherapy;antiemetic cream group was additionally given acupoint application of antiemetic cream 30 min before medication on first-forth day on the basis of control group;Weifuan group was additionally given acupoint injection of metoclopramide(brand name:Weifuan) 30 min before medication on first-forth day on the basis of control group. The clinical efficacy of 3 groups were observed during and 3 days after chemotherapy. RESULTS:After chemotherapy,the total control rates of antiemetic cream group (93.33%)and Weifuan group(90.00%)were apparently higher than the control group(66.67%),with statistical significance(P<0.01 or P<0.05);The total effective rates of TCM syndrome in antiemetic cream group (90.00%) and Weifuan group(83.33%) were apparently higher than in control group(60.00%),with statistical significance(P<0.01 or P<0.05). Therapeutic efficacy of antiemetic cream group was similar to Weifuan group,without any statistical significance(P>0.05). However,8 patients had obvi-ously pain at the injection site in Weifuan group,but tolerated to the drugs basically. CONCLUSIONS:The acupoint application of antiemetic cream combined with intravenous injection of ondansetron is effective and easy to operate in the treatment of nausea and vomiting induced by chemotherapy for stage Ⅱ to Ⅲ of NSCLC without pain,scar and sequela,and easy to be accepted by patients.

Objective To investigate the clinical efficacy of acupoint transdermal administered aniemetic herbal plaster combined with intraveous ondansetron preventing chemotheray -induced vomiting .Methods 98 cases of malignant tumor chemotherapy patients were randomly divided into the treatment group of 49 patients and a control group of 49 patients.The treatment group were acupoint transdermal administered animetic herbal plaster combined with intravenous ondansetron while the control group were intravenously administered ondansetron .The clinical effica-cy of preventing chemotheray-induced vomiting was observed .Results The total effective control rate of the treatment group was 93.9% compared with 79.6% of the control group,the efficacy of the treatment group was significantly greater than that in the control group ( P<0.05 ) .Conclusion The antiemetic herbal plaster exhibited significant effect on preventing chemotherapy-induced vomiting after acupoint transdermal application combined with intravenous ondansetron .

OBJECTIVE: To compare the efficiency of human bone marrow mesenchymal stem cells (hBMSCs) with rat BMSCs (rBMSCs) transfected by modified adenovirus containing fiber 35 (AdF35)-enhanced green fluorescence protein(eGFP). METHODS: We separated hBMSCs and rBMSCs from the bone marrow of humans and rats, respectively, and osteogenesis and adipogenesis were induced. eGFP was carried by modified AdF35, which was transfected to hBMSCs and rBMSCs with different multiplicity of infections (MOIs). Activity of the cells was detected by MTT. The transfected cells were observed under fluorescent microscope. The transfection efficiency was measured by flow cytometer. The expression of coxsackie and adenovirus receptor (CAR) and CD46 mRNA in the cells was inspected by real time PCR. RESULTS: hBMSCs and rBMSCs induced osteogenesis and adipogenesis successfully after being separated from human and rat bone marrow respectively. The activity of the cells was inhibited when MOI was 1,000 PFU/mL. hBMSCs with strong green fluorescence were observed but few rBMSCs were seen under fluorescence microscope 48 h after being transfected by AdF35-eGFP. The transfective efficiency was (84.8±7.1)% and (3.3±1.1)%, respectively. The expression of CD46 was high while that of CAR was low in hBMSCs. The expression of CAR was very high and that of CD46 was low in rBMSCs (P<0.01). CONCLUSION AdF35 may be the ideal vector to carry the target gene to transfect hBMSCs effectively but not to transfect rBMSCs.
Adenoviridae ; genetics ; Animals ; Bone Marrow Cells ; cytology ; metabolism ; Genetic Vectors ; Green Fluorescent Proteins ; biosynthesis ; genetics ; Humans ; Mesenchymal Stem Cells ; cytology ; metabolism ; Rats ; Transfection

As the major part of mesencephalic locomotion region, pedunculopontine tegmental nucleus (PPN) participates in motor initiation, rhythmic and speed regulation. In addition, PPN is regarded as a novel deep brain stimulation target for patients with Parkinson′s disease due to its dramatic effect on the gait disturbance and postural instability. However, PPN also has an important role in muscle tone control and dystonia. This review is aimed at summarizing the involvement of PPN in dystonia, providing fundamental for targeting PPN for treatment of dystonia in the future.

Objective To investigate the expression types of protease-activated receptors (PAR) in human gingival fibroblasts(HGF) and the functions of PAR in periodontitis.Methods Primary HGF were cultured.Reverse transcription PCR(RT-PCR) was used to detect the expression of PAR in HGF.Recombinant gingipain R (rRgp) was applied to HGF.The change of PAR expression on the cell surface was analyzed by real-time quantitative RT-PCR,and enzyme-linked immunosorbent assay (ELISA) was used to detect the change of the interleukin(IL)-6 production from HGF.The results of RT-PCR and ELISA were statistically analyzed using the two independent samples t-test of SPSS10.0 software.Results HGF expressed PAR-1 and PAR-3.The expression of PAR-1 and PAR-3 changed after two rRgp treatment with HGF cells.The relative expression of PAR-1 was decreased from 1.04 ± 0.31 to 0.67 ± 0.11 and 0.31 ±0.11.The relative expression of PAR-3 was decreased from 1.01 ±0.44 to 0.79 ±0.13 and 0.44 ± 0.12 (P ＜ 0.05).The level of IL-6 was increased after rRgp treatment for 8 h.The control group was (18.77 ±4.09) μg/L,the rRgp treatment groups were (179.36 ± 15.81) and (320.56 ± 26.19) μg/L respectively.Conclusions HGF expressed PAR-1 and PAR-3 and were involved in periodontal inflammation.

To explore the process management strategies of standardized residency training based on the development of information management platform, combining with the management practices, this study has sorted out and analyzed process management contents, and integrated process management concepts and contents into the development of information management platform. The development of information management platform taking the process management as the core has contributed to the update and implementation of standardized residency training policies, building of a multi-role interactive bridge, guarantee of information accuracy, and promotion of the construction of quality assurance system, thus providing an effective tool to ensure the quality of training, which is of great significance to cultivate outstanding physicians.

Purpose: Chronic stress and related hormones are key in cancer progression. Peroxisome proliferator-activated receptor γ (PPARγ) and its agonists was reported that inducing anti-tumor effect. However, the function of PPARγ in pro-tumorigenic effects induced by chronic stress in breast cancer remains unknown. Herein, we have characterized a novel role of PPARγ and vascular endothelial growth factor (VEGF)/fibroblast growth factor 2 (FGF2) signals in breast cancer promoted by chronic stress. Materials and Methods: We performed experiments in vivo and in vitro and used bioinformatics data to evaluate the therapeutic potential of PPARγ in breast cancer promoted by stress. Results: Chronic stress significantly inhibited the PPARγ expression and promoted breast cancer in vivo. VEGF/FGF2-mediated angiogenesis increased in the chronic stress group compared to the control group. PPARγ agonist pioglitazone (PioG) injection offset the pro-tumorigenic effect of chronic stress. Moreover, specific β2-adrenergic receptor (β2R) antagonist ICI11-8551 inhibited the effect of chronic stress. In vitro, norepinephrine (NE) treatment had a similar tendency to chronic stress. The effect of NE was mediated by the β2R/adenylate cyclase signaling pathway and suppressed by PioG. PPARγ suppressed VEGF/FGF2 through reactive oxygen species inhibition. Bioinformatics data confirmed that therewas a lowPPARγ expression in breast invasive carcinoma. Lower PPARγ was associated with a significantly worse survival. Conclusion β2R activation induced by chronic stress and related hormones promotes growth and VEGF/FGF2-mediated angiogenesis of breast cancer by down-regulating PPARγ. Our findings hint that β receptor and PPARγ as two target molecules and the novel role for their agonists or antagonists as clinical medicine in breast cancer therapy

Objective: To explore the current residents training of standardized training program and their attitude towards the program, so as to provide suggestions for optimizing the training system. Methods: Questionnaire surveys were carried out among 1 427 resident physicians from 10 hospitals in 7 provinces and cities between March 2017 to May 2017. The investigation covered demographic information, current work and study, training effect and problems. Statistical description and χ² test were used for analysis. Results: Only 18.08% of the residents in standardized training program considered it as unnecessary, while 80% of them agreed with its effectiveness in improving their clinical skills and humanistic quality. 67.29% of them thought of resignation because of low income and high work pressure. Compared with " double track" residents, those of " double-track integration" complained of lower income and higher workload. Conclusions Chinese residents in standardized training program had a positive attitude towards the training system. However, the measures should be improved to prevent from possible occupational burnout. It is suggested that professionalism and professional identity education be improved, and subjective well-being feeling of residents be focused to avoid burnouts.

BACKGROUNDHemifacial spasm (HFS) is a facial nerve disorder characterized by episodic involuntary ipsilateral facial muscle contraction. Information on Chinese patients with HFS has not been well-characterized. This study aimed to evaluate the clinical feature and the treatment status of HFS across China.

METHODSA cross-sectional study including 1003 primary HFS patients had been carried out in 15 movement disorder clinics in China in 2012. The investigated information was acquired from questionnaires and medical records including demographic data, site of onset, aggravating and relieving factors, treatments prior to the investigation, etc.

RESULTSIn this study, the ratio of male to female was 1.0:1.8, the mean age at onset was (46.6 ± 11.5) years. About 1.0% patients were bilaterally affected. The most often site of initial onset was the orbicularis oculi muscle. The most often affected sites were orbicularis oculi, zygomatic, and orbicularis oris muscles. Stress/anxiety and relaxation were most often aggravating and relieving factors, respectively; 2.3% patients had family history, 28.4% cases were combined with hypertension, and 1.4% patients were with trigeminal neuralgia. Botulinum toxin type A (BTX-A) injection was the most commonly used treatment, followed by acupuncture and oral medication. BTX-A maintained the highest repeat treatment ratio (68.7%), while 98.4% patients gave up acupuncture. The mean latency of BTX-A effect was (5.0 ± 4.7) days, the mean total duration of the effect was (19.5 ± 11.7) weeks, and 95.9% patients developed improvements no worse than moderate in both severity and function. The most common side effect was droopy mouth.

CONCLUSIONSThe onset age of HFS in China is earlier than that in western countries. The most often used two treatments are BTX-A injection and acupuncture, while the latter kept the poor repeat treatment ratio because of dissatisfactory therapeutic effect.

Adult ; Botulinum Toxins, Type A ; therapeutic use ; China ; Cross-Sectional Studies ; Female ; Hemifacial Spasm ; diagnosis ; drug therapy ; Humans ; Male ; Middle Aged ; Neuromuscular Agents ; therapeutic use