Let-7 is one of the most widely studied in miRNAs at present. let-7 can inhibit cell proliferation, promote cell differentiation and apoptosis, etc. Reduced expression of let-7 in different human tumor tissues or cells is accompanied by the changes of its target genes expression, which suggests that let-7 is closely linked to cancer.

OBJECTIVE To investigate the HCV infection and the distribution condition of HCV subtype in maintenance hemodialysis patients.METHODS A total of 210 cases with maintenance hemodialysis were recruited into this study.Serum samples were tested for anti-HCV antibodies using ELISA and HCV RNA by RT-PCR.At the same time,samples with anti-HCV IgM positive were identified for their subtype.RESULTS In all cases,91 cases(43.3%) were anti-HCV IgM positive,87 cases(41.4%) were anti-HCV IgG positive,and 105 cases(50.0%) were HCV-RNA positive.Eighty one cases(38.6%) were positive for all HCV infection markers,and 117 cases(55.7%) were positive for at least one marker.Compared that of cases with negative all HCV infection markers,the levels of ALT,AST,LDH and ?-GT in maintenance hemodialysis patients whose at least one marker was positive were significantly higher.Of 91 cases with positive anti-HCV IgM,55 cases(64.4%) were with type 1,9 cases(9.8%) with type 2,13 cases(14.2%) with type 3,and 7 cases(7.6%) with type 6.In addition,3 cases(3.2%) with types 1+6,and 4 cases(4.3%) were with types 1+3.CONCLUSIONS The infectious ratio of HCV in maintenance hemodialysis patients is high,particularly that of type 1 HCV is the highest.

Objective To investigate the serum levels of hepatocyte growth factor (HGF) and granulocyte macrophage-colony stimulating factor (GM-CSF) in patients with psoriasis. Methods Serum samples were collected from 68 patients with psoriasis vulgaris and 42 healthy controls. The serum concentrations of HGF and GM-CSF were measured by a dual antibody sandwich enzyme-linked immunosorbent assay (ELISA). Clinical severity of psoriasis vulgaris was assessed by psoriasis area and severity index (PASI) score. Results Serum concentration of HGF of patients with psoriasis vulgaris in progressive stage (653.35 ? 109.26 pg/mL) was significantly higher than that of the normal controls (353.85 ? 162.63 pg/mL) (P 0.05). The serum concentration of GM-CSF was significantly higher in the patients with psoriasis vulgaris of progressive stage (115.95 ? 25.72 pg/mL) than that in the patients of stable stage (68.28 ? 24.22 pg/mL) (P

Objective To evaluate the therapeutic effects of metformin hydrochloride and rosiglitazone maleate tablets on insulin resistance and androgen concentration in obese and nonobese women with polycystic ovarian syndrome (PCOS).Methods Seventy women with PCOS were enrolled into this study according to the diagnostic criteria of Rotterdam conference (2003).The women with PCOS were divided into the over weight/obese group (body mass index ≥ 25 kg/m2,n =34) and the nonobese group (body mass index＜25 kg/m2,n =36).All women with PCOS took metformin hydrochloride and rosiglitazone maleate tablets twice daily for 3 months.The following indexes were measured before and after the treatment:height,weight,waist circumference,hip circumference,fasting plasma glucose,fasting plasma insulin,luteinizing hormone (LH),follicle stimulating hormone (FSH),total testosterone,free testosterone,follicular number and ovarian area,body mass index,waist to hip ratio,and homeostasis model assessment for insulin resistance (HOMA-IR) were calculated.Results (1) Compared with the nonobese group,dramatic increases in HOMA-IR and follicle number were observed in over weight/obese group(P＜0.05),while LH,LH/FSH,total testosterone,free testosterone showed no significant difference between two groups.(2) After the treatment with metformin hydrochloride and rosiglitazone maleate tablets,weight,body mass index,fasting plasma glucose,fasting plasma insulin,HOMA-IR significantly decreased in two groups (P ＜ 0.05).There were also significant declines of LH,LH/FSH,free testosterone,ovarian area,follicular number,especially in the over weight/ obese women with PCOS (P＜0.05),while total testosterone and FSH did not change significantly.(3) The incidence of gastrointestinal adverse reactions in nonobese and over weight/obese group was 9.3％ and 6.3％ respectively,and no liver toxicity,edema,hypoglycemia and other adverse reactions was observed during treatment.Conclusion Compared with the nonobese group,there were severer insulin resistance and more marked polycystic change of ovary in over weight/obese women with PCOS,while total testosterone and free testosterone showed no significant difference between two gruops.Metformin hydrochloride and rosiglitazone maleate tablets can ameliorate insulin resistance and polycystic ovary,and also reduce body mass index and free testosterone in women with PCOS particularly in the over weight/obese individuals.

Objective To investigate the relationship between platelet-derived growth factor(PDGF)and granulocyte colony-stimulating factor(G-CSF)and psoriasis vulgaris.Methods Sera were taken from60patients with psoriasis vulgaris and42healthy controls.The serum levels of PDGF and G-CSF were measured by a dual antibody sandwich enzyme-linked immunosorbent assay(ELISA).Clinical severity of psoriasis vul-garis was assessed by psoriasis area and severity index(PASI)score.Results The serum PDGF levels were significantly higher in patients with psoriasis vulgaris than those in normal controls(P

This study was aimed to observe the effect of traditional Chinese medicine (TCM)Bu-Shen Gu-Jin Prescription (BSGJP) on the expression and action mechanism of interleukin-1 (IL-1) and tumour necrosis factor-alpha (TNF-α) in knee osteoarthritis (KOA) rabbit model. A total of 76 8-month healthy male New Zealand white rabbits were randomly divided into the control group, model group, TCM BSGJP group and western medicine group, with 19 rabbits in each group. Except the control group, improved Hulth modeling method was used in the animal model establishment. After 8-week consecutive medication, the serum, part of the synovial membrane tissues on the anterior side of the knee joint, articular cartilage and subchondral bone of the medial condyle of the femur were collected. Enzyme-linked immunosorbent assay (ELISA) was used in the content determination of IL-1 and TNF-α. The results showed that the narrowing of the joint space of the western medicine group were in between the model group and the TCM BSGJP group with obvious osteophyte formation. The TCM BSGJP group had slight narrowing of the joint space, mild rough of the articular cartilage surface, and slight osteophyte formation. The contents of IL-1 and TNF-α in the model group were higher than than of the control group (P < 0.01). Both medication groups were lower than the model group (P < 0.01). There was no significant difference between the two medication groups. It was concluded that BSGJP effectively reduced the secretion of IL-1 and TNF-α, delayed the deterioration of cartilage, which provided scientific experimental evidences for the clinical applications.

Objective:To investigate the effect of anastrozole on sex hormone levels and michigan cancer foundation-7 ( MCF-7 ) cell inhibition in postmenopausal women with breast cancer. Methods: Totally 80 women with breast cancer were selected. Among them, 40 cases of patients received the routine management and tamoxifen treatment were included into the control group, and another 40 cases of patients received the routine management and anastrozole treatment were included into the observation group. The changes of sex hormone levels, effects and adverse drug reactions of two groups were compared between basetine and three months after treat-ment, meanwhile, the incidence of MCF-7 cell inhibition between two groups. Results:After treatment, the levels of estradiol( ER) , progesterone(PR) and luteotropic hormone(LH) were lower than baselime, however, the level of testosterone was contrary(P <0. 05). Meanwhile, the level of ER, PR and LH in control group were lower than baselime however, the level of testosterone was cont-ray (P<0. 05). The incidence of MCF-7 cell inhibillion between two grugs at time 72 h were higher than the 48 h, the incedence of MCF-7 cell inhibition in observation group were significantly higher than control groups at time 48 h and 72 h (P<0. 05). The rate of total effective in control group(45. 0%) was lower than the observation group(70. 0%)(P>0. 05). There was no significant differente in the micidence of adverse reactions. Conclusion:Anastrozole used for postmenopausal women with breast cancer has significant effi-cacy, high safety and promising inhibitory effect on MCF-7 cell. It can effectively adjust sex hormone level, reduce occurrence and transfer risks of breast cancer.

Objective:To investigate the effect of anastrozole on sex hormone levels and michigan cancer foundation-7 ( MCF-7 ) cell inhibition in postmenopausal women with breast cancer. Methods: Totally 80 women with breast cancer were selected. Among them, 40 cases of patients received the routine management and tamoxifen treatment were included into the control group, and another 40 cases of patients received the routine management and anastrozole treatment were included into the observation group. The changes of sex hormone levels, effects and adverse drug reactions of two groups were compared between basetine and three months after treat-ment, meanwhile, the incidence of MCF-7 cell inhibition between two groups. Results:After treatment, the levels of estradiol( ER) , progesterone(PR) and luteotropic hormone(LH) were lower than baselime, however, the level of testosterone was contrary(P <0. 05). Meanwhile, the level of ER, PR and LH in control group were lower than baselime however, the level of testosterone was cont-ray (P<0. 05). The incidence of MCF-7 cell inhibillion between two grugs at time 72 h were higher than the 48 h, the incedence of MCF-7 cell inhibition in observation group were significantly higher than control groups at time 48 h and 72 h (P<0. 05). The rate of total effective in control group(45. 0%) was lower than the observation group(70. 0%)(P>0. 05). There was no significant differente in the micidence of adverse reactions. Conclusion:Anastrozole used for postmenopausal women with breast cancer has significant effi-cacy, high safety and promising inhibitory effect on MCF-7 cell. It can effectively adjust sex hormone level, reduce occurrence and transfer risks of breast cancer.

Objective: To investigate the effect of miR-124 on the invasion and migration of esophageal cancer KYSE170 cells by regulating autophagy. Methods: miR-124 mimic was transfected into esophageal cancer KYSE170 cells. Transwell assay was used to detect the change of invasion and migration ability of cells. Dual luciferase reporter gene assay was used to verify the targeted regulation of BECN1 (Beclin1) by miR-124, and Western blotting was used to analyze the expressions of BECN1, P62 and LC3 protein. siRNA targeting BECN1 was transfeted into KYSE170 cells, and then the cell invasion and migration ability was calculated by Transwell assay. The expressions of BECN1, P62 and LC3 protein were detected by Western blotting. miR-124 mimic and BECN1 over-expression plasmid were co-transfected into KYSE170 cells, and then Transwell assay was used to detect the changes of cell invasion and migration ability, and Western blotting to examine the expression levels of autophagy-related gene. Results: The invasion and migration ability of KYSE170 cells were significantly inhibited after transfection with miR-124 mimic (All P<0.05). The expression of autophagyrelated protein P62 was increased, and the expression of BECN1 and LC3 was significantly decreased (All P<0.01); in addition, the activity of luciferase reporter gene was also significantly reduced (P<0.01). Silencing BECN1 expression inhibited the invasion and migration of esophageal cancer KYSE170 cells (P<0.01). However, after co-transfection with BECN1 over-expression plasmids, the effects of miR-124 mimic on the autophagy, invasion and migration of esophageal carcinoma KYSE170 cells were significantly weakened (P<0.01), it was also accompanied with lower P62 expression, and higher LC3 expression (P<0.01). Conclusion: miR-124 mimic can inhibit the invasion and migration of esophageal carcinoma cells. The mechanism may be related to the autophagy-related gene BECN1 expression.

[Abstract] Objective: To investigate the regulating effects of miR-92b on the expression of EZH2 (enhancer of zeste homolog 2) gene and the proliferation and invasion abilities of esophageal cancer (EC) cells. Methods: Fifteen cases of esophageal cancer tissues that preserved in the research center of the Fourth HospitalAffiliated to Heibei Medical University from January 2016 to January 2017 were selected for this study. The bioinformatics tool was used to predict the possible miRNAs that might target EZH2. The mimics of predicted miRNAs were transfected into human esophageal carcinoma cell lines Eca109, respectively. Then the regulation effect of miRNAs on EZH2 gene expression was validated by real-time PCR, Western blotting and dual luciferase reporter experiment. In the meanwhile, EZH2 over-expression plasmids were co-transfected into esophageal carcinoma Eca109 cells, and the effects of miRNAs and EZH2 expression changes on the proliferation, apoptosis , invasion and migration of esophageal carcinoma cells were detected by CCK-8 method, Flow Cytometry, Transwell Invasion and migration assay, respectively. Results: Bioinformatics analysis showed that miR-92b, let7a and miR-25 could combine with potential binding sites at 3’-terminal non-translation region of EZH2 gene. Real-time PCR results showed that only miR-92b was able to regulate the expression of EZH2, and miR-92b was negatively correlated to EZH2 in esophageal cancer (P<0.01). Compared with mimic-NC, the expression of EZH2 mRNA, protein and luciferase activity in Eca109 cells after miR-92b mimic transfection was significantly down-regulated (both P<0.01). However, miR-92b mimic transfection had no effect on the apoptosis of Eca109 cells. Moreover, the proliferation, invasion and migration of Eca109 cells were significantly inhibited after transfection with miR-92b-mimic (P<0.01). In addition, after co-transfection with EZH2 over-expression plasmids, the effects of miR-92b-mimic on the proliferation, invasion and migration of Eca109 cells were significantly weakened (P<0.01). Conclusion: miR-92b can inhibit the proliferation,invasionandmigrationofesophagealcarcinomacells,anditsmechanismmayberelatedtoitstargetregulationofEZH2. ··