1.Effects of Explosion Sound Waves on Auditory Induced Potential & Cochlea SDH, MDH Activities in Guinea Pigs
Suoqiang ZHAI ; Rui GU ; Linghan HE
Journal of Third Military Medical University 1983;0(03):-
This paper is to report the results of the experimental study on the effects of the explosion sound waves on the hearing power and the activities of succinic dehydrogenase (SDH) and malic dehydrogenase (MDH) of the cochlea tissues in guinea pigs. The alterations of the hearing power and the activities of the two enzymes were monitored with auditory physiological and histochemical methods respectively. The results of the experiment were as follows:The activities of both SDH and MDH decreased significantly (P
2.Influence of Vestibular Nystagmus on Optokinetic Nystagmus
Linghan HE ; Dingrong ZHOU ; Suoqiang ZHAI ; Weiheng XU
Journal of Third Military Medical University 1983;0(04):-
The interference of optokinetic nystagmus from vestibular nystagmus was evaluated in 10 patients with a pre-existing spontaneous peripheral vestibular nystagmus, and in 9 normal subjects, in whom vestibular nystagmus was induced with 10 times of 60?sec constant speed count-clockwise rotation. The examinees of both groups were all subjected to horizontal clockwise and counter-clockwise optokinetic stimulation(target speed 60?/sec, frequency 2 Hz).It was found that vestibular nystagmus exerted no significant modifying effect on the eye velocity of the slow phas? of optokinptic nystagmus and that no favourablp evid?cs was demonstrated to support the algebraic summation hypothesis.
3.Exclusive gene mapping on a Chinese familial IgA nephropathy family
Shumei SHI ; Linghan GAO ; Xuezhi ZHAO ; Aiping ZHANG ; Yaohai DING ; Yanxia WANG ; Guoyin FENG ; Lin HE
Chinese Journal of Nephrology 2011;27(2):77-81
Objective To initially map the gene responsible for autosomal dominant familial IgA nephropathy of a Chinese family by exclusive the five loci that had been reported with linkage analysis.Methods The genetic pattern of the familial IgA nephropathy was identified and the genomic DNA was extracted from the blood samples collected from the family members.Short tandem repeat (STR) inside the loci that had been reported was selected,such as 2q36,3p23-24,4q26-31,6q22-23,17q12-22,and the data with two-point linkage analysis were performed.Results Autosomal dominant inheritance pattern was demonstrated in phenotypes of the family and there was no linkage relationship in the above five loci of chromosomes because the maximum two-point LOD score was 0.39 at D17S1868.Conclusion Following exclusion of the loci which had been reported,there are other new pathopoiesis loci of FIgAN and it reveals that FIgAN has the genetic heterogeneity according to initial result at the same time.
4.Analysis of TGFBI gene mutation in a pedigree affected with corneal dystrophy.
Anli SHU ; Gen LI ; Hai LUO ; Juan HE ; Xiaowen HU ; Jiayi SUN ; Ying QING ; Linghan GAO ; Juan ZHANG ; Chao YANG ; Lin HE ; Chunling WAN
Chinese Journal of Medical Genetics 2018;35(5):672-674
OBJECTIVETo detect potential mutation in a large Chinese pedigree affected with congenital corneal dystrophy.
METHODSTwo patients from the pedigree were subjected to whole exome sequencing to determine the candidate gene. Suspected mutation was verified in 13 additional members by directional Sanger sequencing. Ccorrelation between genotype and phenotype was explored.
RESULTSA missense mutation, c.1877A>C (p.His626Pro), was detected in exon 14 of the TGFBI gene in 8 patients from the pedigree, but not in five unaffected members and 100 unrelated healthy controls. Respectively, the mutation was predicted as "affecting protein function", "probably damaging" and "disease causing" by SIFT, PolyPhen-2 and MutationTaster.
CONCLUSIONThe c.1877A>C mutation of the TGFBI gene probably underlies the disease in this pedigree.
5.Level of peripheral blood circulating tumor cells in lung cancer patients detected by CI-101 and its clinical significance
Linghan TIAN ; Ling ZHU ; Xin LIU ; Jingwen YE ; Jiahui HE ; Xicai WANG
Journal of International Oncology 2020;47(4):199-204
Objective:To explore the correlations between circulating tumor cells (CTCs) level in peripheral venous blood and clinicopathological characteristics and biomarkers of lung cancer patients using CI-101 cell search immunomagnetic bead enrichment technology combined with fluorescent cytochemical staining.Methods:Blood samples were collected from 100 patients with first-diagnosed lung cancer treated in Department of Thoracic Surgery and Department of Cardiothoracic Surgery of Yunnan Cancer Hospital from March 2014 to September 2014, 40 patients with lung benign tumor (all confirmed by pathological biopsy) and 30 healthy volunteers from the physical examination center. CTCs in peripheral blood were enriched by CI-101 cell search immunomagnetic bead, the morphology of CTCs was analyzed by immunocytofluorescence technique, and tumor cells were identified using HE cell staining method. The recovery rate, sensitivity and specificity of CI-101 cell search instrument for CTCs were detected. The difference of positive rate of CTCs in peripheral blood among lung cancer patients, lung benign tumor patients and healthy volunteers was compared. The relationship between the positive rate of CTCs and the clinicopathological characteristics of patients with lung cancer was analyzed. The correlations between CTCs and serum tumor markers were analyzed by coefficient of contingency in patients with lung cancer and lung benign tumor.Results:The recovery rate of CTCs by CI-101 cell search instrument was 72.0%-89.0%, and there was a significant linear correlation between the number of recovered cells and the number of incorporated cells. The correlation coefficient r=0.998 ( P<0.001), the linear regression equation was y=0.781 x+ 11.307, the sensitivity was 85.0%, and the specificity was 71.4%. The positive rate of CTCs in lung cancer patients (85.0%, 85/100) was higher than that in lung benign tumor patients (15.0%, 6/40) and healthy volunteers (46.7%, 14/30) ( χ2=62.798, P<0.001). The positive rate of CTCs in lung cancer patients was correlated with TNM stage ( χ2=19.059, P<0.001), tumor size ( χ2=13.830, P<0.001) and distant metastasis ( χ2=6.005, P=0.014). Coefficient of contingency analysis showed that the positive of CTCs was positively correlated with serum tumor markers CEA ( φ=0.217, P=0.011), CA125 ( φ=0.198, P=0.020), CA199 ( φ=0.169, P=0.049), CA742 ( φ=0.186, P=0.037) and cytokeratin 19 fragment ( φ=0.461, P<0.001) in patients with lung cancer and lung benign tumor. Conclusion:The application of CI-101 cell search instrument combined with immunomagnetic bead method can successfully enrich CTCs in peripheral venous blood of lung cancer patients. The positive rate of CTCs in patients with lung cancer has obvious correlation with tumor size, TNM stage, distant metastasis, serum tumor markers. It can be used as an auxiliary indicator for monitoring the condition of lung cancer patients.
6. lncRNA and small cell lung cancer
Chao MING ; Rui HE ; Yuan SUN ; Linghan TIAN ; Guangqiang ZHAO
Journal of International Oncology 2019;46(10):620-623
The incidence and mortality of lung cancer rank first, and small cell lung cancer (SCLC) accounts for about 15% of lung cancer cases. In recent years, it has been found that long non-coding RNA (lncRNA) (TUG1, CCAT2, PVT1, HOTTIP and HOTAIR) plays a significant role in the development of SCLC. lncRNA can regulate the expression of related genes at the transcriptional, posttranscriptional and epigenetic levels, and it can infulence proliferation, invasion, metastasis and chemotherapy resistance of SCLC.