Objective To study the association of neuregulin 1(NRG1)gene SNPrs2954041 polymorphisms with schizophrenia and cognitive function.Methods 70 psychiatric patients were selected as observation group, 70 healthy persons were selected as control group.NRG1 gene SNPrs2954041 polymorphism was detected,and the patients'cognitive function and neurological NSS score were evaluated.Results The differences of SNPrs2954041 polymorphism genotype and allele frequencies between the two groups were statistically significant(χ2 =35.412,29.341, all P =0.000).The differences in genotype and allele frequencies between the male observation patients and male control group were statistically significant(χ2 =24.571 ,18.391 ,all P =0.000).The differences in genotype and allelefrequencies between female the observation patients and female control group were statistically significant (χ2 =14.145,13.024,all P =0.000).The difference of memory understanding among the three groups was statistically sig-nificant(F =3.781,P =0.031).The difference of digit span,correct number,errors number,errors continued num-ber,non -sustained errors number in three groups showed no significant differences(F =0.702,0.125,0.089, 0.692,0.113,P =0.498,0.882,0.927,0.512,0.748),the difference of neurological soft signs score of T/T geno-type[(6.79 ±0.55)points],T/G genotype[(6.88 ±0.51 )points]and G/G genotype[(6.53 ±0.39)points]in schizophrenia group was not statistically significant(F =0.142,P =0.843).Conclusion NRG1 gene SNPrs2954041 polymorphism was related to schizophrenia and cognitive function in patients with schizophrenia.

Objective To define the application value of HBV DNA detection on HBsAg-negative blood donors and assess the necessity for nucleic acid detection.Methods Real-time PCR was used to detect HBV DNA on HBsAg negative blood donors.Pools of eight donor samples were used for NAT testing.Viruses were concentrated by centrifugation and the viral DNA extraction was performed using magnetic beads.If HBV DNA wag positive,serological indicators including HBsAg,anti-HBs,HBeAg, anti-Hbe,total anti-HBc was further detected.Results The HBV DNA detection limit was 25 U/ml.There were four HBV DNA positive cases among 23 225 specimens.and the detection rate was 0.17‰ The further serological examination showed anti-Hbe(+),anti-HBc(+) in the two cases and anti-HBc(+) in one case and anti-Hbs(+),anti-HBc(+)in 1 case.The viral load can range form 50 to 200 U/ml. Conclusions The results indicate that there is false negative possibility in blood screening by ELISA.It is necessary to employ anti-Hbe screening or NAT to blood donors screening.